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In vitro growth performance of Psidium guajava and P. guineense plantlets as affected by culture medium formulations

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Abstract

The adjustment of protocols for the in vitro culture is fundamental for the conservation and application of biotechnological techniques of Psidium spp. This study has been aimed to determine the most appropriate culture medium formulation for the in vitro culture of Psidium guajava ‘Paluma’ (cv. GP) and a wild relative species, P. guineense. Seedlings of three accessions (Y93, Y95, and Y97) of P. guineense and cv. GP, previously germinated in vitro, were inoculated in the JADS, MS, and WPM culture media. After 110 days of culture, the growth morpho-physiological and nutritional parameters were assayed in the guava shoots. The use of JADS culture medium led to the highest growth in both guava species. The largest stomatal index was observed in the abaxial leaf surface of the plants grown in JADS, except for the accession Y97, which showed higher values in MS. The nutritional analysis revealed that the accumulation of nutrients in P. guineense shoots did not coincide with the composition of the culture media. Present studies conclude that the in vitro growth of the plants was influenced by the composition of the culture medium, being JADS the most indicated for the micropropagation of these guava plant species.

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Acknowledgements

The authors would like to thank the National Council for Scientific and Technological Development (CNPq) (Brazil), Coordination for Improvement of Higher Education Personnel (CAPES, Brazil, Financial Code 01), Research Support Foundation of the State of Minas Gerais (FAPEMIG) (Belo Horizonte, MG, Brazil), and the State University of Bahia (UNEB) (Juazeiro, BA, Brazil) for the financial support.

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Authors and Affiliations

  1. Centro de Ciências Agrárias, Universidade Federal da Paraíba, Campus II, Areia, PB, 58.397-000, Brazil

    Márcia Adriana Carvalho dos Santos & Mailson Monteiro do Rêgo

  2. Departamento de Tecnologia e Ciências Sociais, Universidade do Estado da Bahia, Campus III, São Geraldo, Juazeiro, BA, 48900-000, Brazil

    Manoel Abílio de Queiróz

  3. Departamento de Biologia Vegetal, Laboratório de Cultura de Tecidos/BIOAGRO, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, 36570-900, Brazil

    Márcia Adriana Carvalho dos Santos, Duanny Thais Rodrigues Caproni, André Ferreira Santos & Wagner Campos Otoni

  4. Laboratório de Solos, Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo (IFES), Campus Itapina, Colatina, ES, 29717-000, Brazil

    Otto Herbert Schuhmacher Dietrich

  5. Instituto de Ciências Biológicas, Universidade Federal de Jataí, Campus Jatobá, Cidade Universitária, Jataí, GO, 75801-615, Brazil

    Diego Ismael Rocha

  6. Departamento de Agricultura, Universidade Federal da Paraíba, Campus III, Bananeiras, Paraíba, 58220-000, Brazil

    Diego Silva Batista

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  1. Márcia Adriana Carvalho dos Santos
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  9. Wagner Campos Otoni
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Correspondence to Wagner Campos Otoni.

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dos Santos, M.A.C., do Rêgo, M.M., de Queiróz, M.A. et al. In vitro growth performance of Psidium guajava and P. guineense plantlets as affected by culture medium formulations. Vegetos 33, 435–445 (2020). https://doi.org/10.1007/s42535-020-00125-6

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