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Characterization of rhizobacterial isolates from Brassica juncea for multitrait plant growth promotion and their viability studies on carriers

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Abstract

The beneficial microbes colonizing the plant root, designated as plant growth promoting rhizobacteria (PGPR), increase the crop productivity and present an attractive and promising way to substitute chemical fertilizers, pesticides, and other harmful supplements. Hence, the present study is envisaged to identify, screen and characterize PGPR from rhizospheric soil of Brassica juncea (Indian mustard). Three sites out of major mustard producing areas under Allahabad in Eastern Uttar Pradesh, India were targeted. Total 20 bacterial isolates were identified and purified from these regions based on occurrence percentage and they were further tested in vitro for plant growth promoting (PGP) traits. Among 20 isolates, only two solubilized phosphate, three isolates produced hydrogen cyanide, 14 assimilated symbiotic nitrogen, two showed siderophore production and nine produced indole-3-acetic acid. Based on PGP tests, three potential isolates were selected for plant growth promotion. Further, this study also investigates the potential use of agricultural wastes as carrier materials, viz. charcoal, sawdust, wheat bran and rice husk, for preparation of bioformulations (or biofertilizers) using three PGPR isolates for viability assessment.

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Acknowledgements

Authors are thankful to Director MNNIT Allahabad for providing necessary facilities. The services and facilities provided by Central Instrumentation Facility under Department of Biotechnology, MNNIT Allahabad are also acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Allahabad, UP, 211004, India

    Kanchan Vishwakarma, Durgesh K. Tripathi & Shivesh Sharma

  2. Department of Biotechnology, Himalayan Institute of Biosciences, Swami Rama Himalayan University, Jollygrant, Dehradun, India

    Vivek Kumar

  3. Amity Institute of Organic Agriculture, Amity University, Noida, India

    Durgesh K. Tripathi

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  1. Kanchan Vishwakarma
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  2. Vivek Kumar
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Correspondence to Shivesh Sharma.

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Vishwakarma, K., Kumar, V., Tripathi, D.K. et al. Characterization of rhizobacterial isolates from Brassica juncea for multitrait plant growth promotion and their viability studies on carriers. Environmental Sustainability 1, 253–265 (2018). https://doi.org/10.1007/s42398-018-0026-y

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