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Plant growth promotion and biological control of Sclerospora graminicola in pearl millet by endophytic Streptomyces spp.

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Abstract

Endophytic Streptomyces spp. were isolated from pearl millet roots and analyzed for growth characteristics on three different media viz., Streptomyces (S) media, casein starch agar and actinomycetes media. Proteolytic activity of Streptomyces was evaluated by using cell free extracts, 23 isolates were found positive and further tested for their inhibition effects on downy mildew pathogen. Cell-free extract and the mycelial mats were used as seed soaking and seed dressing treatments, respectively. Twenty days old susceptible coleoptile seedlings were artificially inoculated with Sclerospora graminicola pathogen for three consecutive days. The downy mildew suppressing ability (30%) and the disease protection (63%) were observed in Streptomyces spp. Isolates S6-19 and S4-19 offered 63% and 56% disease protection against downy mildew respectively and also promoted the vegetative and reproductive growth of the plant. Thus, endophytic Streptomyces spp. proved as an effective possible biocontrol agent in enhancing the disease resistance.

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Acknowledgements

Author PHG greatly acknowledges the University Grant Commission (UGC), New Delhi, India for financial assistance.

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This research was supported by the University Grant Commission (UGC) for financial support.

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Authors and Affiliations

  1. Department of Biotechnology, University of Mysore, Mysore, Karnataka, 570 006, India

    H. G. Pushpalatha, N. Geetha & H. Shekar Shetty

  2. Maharani’s Science College for Women, Mysore, Karnataka, 570 001, India

    H. G. Pushpalatha

  3. Department of Biochemistry, CFTRI, Mysore, Karnataka, 570 006, India

    J. Naveen & G. Hithamani

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  1. H. G. Pushpalatha
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  2. J. Naveen
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  3. N. Geetha
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The study carried out and manuscript was written with contributions from the authors PHG and NJ, manuscript edited by GN, HG and HSS. All authors approved the final version of the manuscript.

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Correspondence to H. G. Pushpalatha.

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Pushpalatha, H.G., Naveen, J., Geetha, N. et al. Plant growth promotion and biological control of Sclerospora graminicola in pearl millet by endophytic Streptomyces spp.. Indian Phytopathology 76, 521–530 (2023). https://doi.org/10.1007/s42360-023-00616-x

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