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Evaluation of rice genotypes against varying population densities of root-knot nematode Meloidogyne graminicola identifies potential sources for resistance breeding

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Abstract

Rice root-knot nematode (RRKN) Meloidogyne graminicola is an important pest of rice. Confirming resistance response under economic threshold level (ETL) and above is advantageous for determining the resistance durability. Here, five known resistant rice genotypes were evaluated under differential inoculum densities of RRKN using Pluronic gel and soil; and performance was assessed after completion of one and two successive nematode generations. The genotypes Vandana, Suraksha, Phule Radha and EK 70 showed reduction in root-galling, endoparasite establishment, eggmass development and eggs per eggmass under all inoculum densities tested in vitro and in soil. However, cv. Khalibagh showed resistance against all inoculum levels in vitro, but succumbed to infection at higher inoculum densities in soil. This approach of resistance durability confirmation established utility of the four promising rice genotypes as resistant donor parents in future breeding programs.

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Availability of data and material

The datasets analyzed during the present study are available from the corresponding author on request.

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Funding

The authors acknowledge DBT COE project BT/PR-18924/COE/34/48/2017 for providing necessary funding. The role of funding body does not include data analysis, interpretation and manuscript writing.

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Author notes

  1. Alkesh Hada and Ankita Raj: equal contribution authors.

Authors and Affiliations

  1. Division of Nematology, ICAR- Indian Agricultural Research Institute, New Delhi, India

    Alkesh Hada, Ankita Raj, Bhupal Hatzade & Uma Rao

  2. Department of Agricultural Entomology, College of Agriculture, Uttar Banga Krishi Viswavidyalaya, Dakshin Dinajpur, West Bengal, India

    Victor Phani

  3. State Level Biotechnology Centre, Mahatma Phule Krishi Vidyapeeth, Rahuri, Maharashtra, India

    Shailesh D. Kumbhar

  4. Division of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi, India

    Ranjith Ellur

Authors
  1. Alkesh Hada
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Contributions

Conceptualization: UR, RE; methodology: AH, AR; contribution of experimental materials: SDK; formal analysis and investigation: AR, AH, BH, VP; writing—original draft preparation: VP, UR, RE; writing—review and editing: VP, UR; funding acquisition: UR; resources: UR; supervision: UR, VP.

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Correspondence to Uma Rao.

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Hada, A., Raj, A., Phani, V. et al. Evaluation of rice genotypes against varying population densities of root-knot nematode Meloidogyne graminicola identifies potential sources for resistance breeding. Indian Phytopathology 75, 75–82 (2022). https://doi.org/10.1007/s42360-021-00429-w

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