Abstract
Blast disease caused by a filamentous heterothallic ascomycetous fungus Magnaporthe oryzae is one of the most devastating diseases of rice crop in different parts of the world. A variety of DNA markers are used for understanding the diversity and virulence spectrum of this fungus. In our current investigation, 21 simple sequence repeat markers are taken to analyse genetic diversity among 72 M. oryzae isolates obtained from different geographical locations of Karnataka, India. Results showed that there is significant variation among the isolates of M. oryzae with 92 polymorphic bands with an average of 4.38 bands for each marker. Average locus heterozygosity and polymorphic information content (PIC) of total markers were 0.73 and 0.60 respectively; Cluster analysis interestingly indicated a correlation of grouping with the geographical boundaries. We have also plotted virulence spectrum for each group and found out mixed types of reaction pattern in each group. SSR based genetic diversity of the pathogen is evolved as per the geographical boundaries and the extent of diversity might help the pathogen significantly in adapting to the changed environment. Hence during breeding program locus heterozygosity, PIC and virulence spectrum have to be considered for obtaining stable resistance cultivars.
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Acknowledgements
We thank University Grants Commission, New Delhi for the financial support by sanctioning Major Research Project (F. No. 41-408/2012 (SR) dated July 2012) to carry out this investigation. Authors are also grateful to Department of Plant Pathology, GKVK, UAS, Bangalore for providing instrumentation facility to carry out molecular work. We are also thankful to Yuvaraja’s College, University of Mysore, Mysuru for providing facilities for carrying out this research work.
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Jagadeesh, D., Kumar, M.K.P., Amruthavalli, C. et al. Genetic diversity of Magnaporthe oryzae, the blast pathogen of rice in different districts of Karnataka, India determined by simple sequence repeat (SSR) markers. Indian Phytopathology 73, 713–723 (2020). https://doi.org/10.1007/s42360-020-00257-4
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DOI: https://doi.org/10.1007/s42360-020-00257-4