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Electrospinning of a sandwich-structured membrane with sustained release capability and long-term anti-inflammatory effects for dental pulp regeneration

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Abstract

Current electrospun membranes used for pulp capping still lack the sustained-release capability and long-term anti-inflammatory effects that are favorable for dental pulp regeneration. In this work, a single-layered poly(lactic acid) (PLA) electrospun membrane loaded with amorphous calcium phosphate (ACP) and aspirin (PLA/ACP/Aspirin membrane, i.e., PAA membrane) is sandwiched between two poly(lactic-co-glycolic acid) (PLGA) electrospun membranes as a novel sandwich-structured PLGA and PAA composite electrospun membrane (PLGA-PAA membrane) to resolve the need for sustained-release design and anti-inflammatory effects. Contact angle measurements indicate that the PLGA-PAA membrane is more hydrophilic than the PAA membrane. An in vitro release study reveals that PLGA membranes coated on PAA membrane could slightly slow down ion release, while significantly prolonging aspirin release. We also co-cultured membranes with dental pulp stem cells (DPSCs) and human monocytic THP-1 cells to evaluate their osteogenic ability and anti-inflammatory effects, respectively. Compared with the PAA membrane, the PLGA-PAA membrane promotes cell adhesion, proliferation, and osteogenic differentiation. A prolonged anti-inflammatory effect of up to 18 days is also observed in the PLGA-PAA group. The results suggest a promising strategy for fabricating an electrospun membrane system with controlled release capabilities and long-term anti-inflammatory effects for use as pulp-capping material for regeneration of the dentin-pulp complex.

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Acknowledgements

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (Nos. 11872097, 82074463, 11827803, and U20A20390), the National Key R&D Program of China (No. 2020YFC0122204), the 111 Project (No. B13003), and the International Joint Research Center of Aerospace Biotechnology and Medical Engineering, Ministry of Science and Technology of China.

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Authors and Affiliations

  1. Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100083, China

    Fenghe Yang, Jiangxue Wang, Xiaoyu Li, Zhenzhen Jia & Xufeng Niu

  2. Department of Burn Plastic Surgery, 80th Army Hospital of PLA, Weifang, 261021, China

    Qiang Wang

  3. Department of Hand Surgery, 971st Army Hospital of PLA, Qingdao, 266000, China

    Dazhi Yu

  4. Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, 100700, China

    Jinyu Li

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  1. Fenghe Yang
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  2. Jiangxue Wang
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  3. Xiaoyu Li
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  4. Zhenzhen Jia
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  5. Qiang Wang
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Contributions

FHY and XFN contributed to conceptualization; FHY, JXW, XYL, and ZZJ contributed to methodology; FHY contributed to investigation; FHY contributed to writing—original draft; QW, DZY, JYL, and XFN contributed to writing—review & editing; XFN contributed to funding acquisition; XFN provided the resources; JYL and XFN contributed to supervision.

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Correspondence to Jinyu Li or Xufeng Niu.

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This study does not contain any studies with human or animal subjects performed by any of the authors.

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Yang, F., Wang, J., Li, X. et al. Electrospinning of a sandwich-structured membrane with sustained release capability and long-term anti-inflammatory effects for dental pulp regeneration. Bio-des. Manuf. 5, 305–317 (2022). https://doi.org/10.1007/s42242-021-00152-5

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