Abstract
Ten plant essential oils (PEOs) were used for evaluating the antifungal activity against Botrytis cinerea through the volatile and contact methods. Moreover, six essential oils of the six plants including cinnamon, rosemary, ginger, ajwain, cumin, and garlic at concentration of 25 μL/mL, which showed the higher inhibition of the mycelia growth of the pathogen were selected for further experiments. Contact and volatile phase effects of these six PEOs at the concentrations of 0, 10, 15, 20, 25, and 35 μL/mL were evaluated on mycelia growth and spore germination of the pathogen in vitro. The essential oils of cinnamon, ajwain, cumin, and garlic (concentrations higher than 20 μL/mL), which indicated the highest inhibition of pathogen isolates growth were selected for further studies in the greenhouse. Volatile phase effects of the PEOs were consistently found to be more effective on the fungal growth than the contact phase. The four used PEOs inhibited the growth of pathogen and the development of grey mould on the tomato plants under the greenhouse conditions. However, none of the treatments affected the plants height and fresh and dry weights. This study aims to indicate the PEOs can be considered as an alternative to fungicides for protecting the tomato plants against B. cinerea.
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References
Abed-Ashtiania F, Arzanlou M, Nasehi A, Kadir J, Vadamalai G, Azadmard-Damirchi S (2018) Plant tonic, a plant-derived bioactive natural product, exhibits antifungal activity against rice blast disease. Ind Crops Prod 112:105–112. https://doi.org/10.1016/j.indcrop.2017.11.013
Aguilar-González AE, Palou E, López-Malo A (2015) Antifungal activity of essential oils of clove (Syzygium aromaticum) and/or mustard (Brassica nigra) in vapor phase against gray mold (Botrytis cinerea) in strawberries. Innov Food Sci Emerg Technol 32:181–185. https://doi.org/10.1016/j.ifset.2015.09.003
Alok S, Jain SK, Verma A, Kumar M, Mahor A, Sabharwal M (2014) Herbal antioxidant in clinical practice: a review. Asian Pac J Trop Biomed 4(1):78–84. https://doi.org/10.1016/s2221-1691(14)60213-6
Al-Taisan WAA, Bahkali AH, Elgorban AM, El-Metwally MA (2014) Effective influence of essential oils and microelements against Sclerotinia sclerotiorum. Int J Pharmacol 10(5):275–281. https://doi.org/10.3923/ijp.2014.275.281
Barrera-Necha LL, Bautista-Bańos S, Flores-Moctezuma HE, Rojas Estudillo A (2008) Efficacy of essential oils on the conidial germination, growth of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc and control of postharvest diseases in papaya (Carica papaya L.). Plant Pathol J 7(2):174–178. https://doi.org/10.3923/ppj.2008.174.178
Barrera-Necha LL, Garduno-Pizana C, Garcia-Barrera LJ (2009) In vitro antifungal activity of essential oils and their compounds on mycelial growth of Fusarium oxysporum f. sp. gladioli (Massey) Snyder and Hansen. Plant Pathol J 8(1):17–21. https://doi.org/10.3923/ppj.2009.17.21
Bhagwat MK, Datar AG (2014) Antibacterial activity of herbal extracts against five plant pathogenic bacteria. Arch Phytopathol 47(7):892–899. https://doi.org/10.1080/03235408.2013.825398
Božik M, Cisarova M, Tancinova D, Kourimska L, Hleba L, Kloucek P (2017) Selected essential oil vapors inhibit growth of Aspergillus spp. in oats with improved consumer acceptability. Ind Crop Prod 98:146–152. https://doi.org/10.1016/j.indcrop.2016.11.044
Brnawi WI, Hettiarachchy NS, Horax R, Kumar-Phillips G, Seo HS, Marcy J (2018) Comparison of cinnamon essential oils from leaf and bark with respect to antimicrobial activity and sensory acceptability in strawberry shake. J Food Sci 83:475–480. https://doi.org/10.1111/1750-3841.14041
Chen X, Wang Y, Gao Y, Gao T, Zhang D (2019) Inhibitory abilities of Bacillus isolates and their culture filtrates against the gray mold caused by Botrytis cinerea on postharvest fruit. Plant Pathol J 35(5):425–436. https://doi.org/10.5423/PPJ.OA.03.2019.0064
Chu YL, Ho WC, Ko WH (2006) Effect of Chinese herb extracts on spore germination of Oidium murrayae and nature of inhibitory substance from Chinese rhubarb. Plant Dis 90(7):858–861. https://doi.org/10.1094/PD-90-0858
Edris AE, Farrag ES (2003) Antifungal activity of peppermint and sweet basil essential oils and their major aroma constituents on some plant pathogenic fungi from the vapour phase. Nahrung 47:117–121. https://doi.org/10.1002/food.200390021
Esterio M, Munoz G, Ramos C, Cofre G, Estevez R, Salinas A, Auger J (2011) Characterization of Botrytis cinerea isolates present in Thompson seedless table grapes in the central valley of Chile. Plant Dis 95:683–690. https://doi.org/10.1094/PDIS-04-10-0298
Etebarian HR, Sholberg PL, Eastwell KC, Sayler RJ (2005) Biological control of apple blue mold with Pseudomonas fluorescens. Can J Microbiol 51:591–598. https://doi.org/10.1139/w05-039
Farhang V, Amini J, Javadi T (2015) Effect of garlic essential oil, Mancozeb and Metalaxyl-Mancozeb on three species of Phytophthora, causal agent of plants. Biol Control Pests Plant Diseases 4(1):47–56. https://doi.org/10.22059/jbioc.2015.54876
Herrera-Téllez VI, Cruz-Olmedo AK, Plasencia J, Gavilanes-Ruíz M, Arce-Cervantes O, Hernández-León S, Saucedo-García M (2019) The protective effect of Trichoderma asperellum on tomato plants against Fusarium oxysporum and Botrytis cinerea diseases involves inhibition of reactive oxygen species production. Int J Mol Sci 20: 2007. https://doi.org/10.3390/ijms20082007
Hyldgaard M, Mygind T, Meyer RL (2012) Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol 3:12. https://doi.org/10.3389/fmicb.2012.00012
Inouye S, Tsuruoka T, Watanabe M, Takeo K, Akao M, Nishiyama Y, Yamaguchi H (2000) Inhibitory effect of essential oils on apical growth of Aspergillus fumigatus by vapour contact. Mycoses 43:17–23. https://doi.org/10.1046/j.1439-0507.2000.00538.x
Jalali H, Ebrahimi L, Etebarian HR (2021) Biocontrol of tomato gray mold disease by Trichoderma harzianum and Bacillus subtilis. J Crop Prot 10 (4): 647-657.
Jham GN, Dhingra OD, Jardim CM, Valente VMM (2005) Identification of the major fungitoxic component of cinnamon bark oil. Fitopatol Bras 30:404–408. https://doi.org/10.1590/S0100-41582005000400011
Jiang Z, Jiang H, Xie P (2013) Antifungal activities against Sclerotinia sclerotiorum by Cinnamomum cassia oil and its main components. J Essent Oil Res 25(6):444–451. https://doi.org/10.1080/10412905.2013.782475
Khodadadi Shahraki K, Khan Ahmadi M, Alavi Hejazi S (2016) Antifungal effect of essential oils of three species of thyme in biocontrol of Botrytis cinerea and Alternaria infectoria. The first international Iran food industry congress, https://civilica.com/doc/479853/
Khosravi AR, Salehi RM, Yahyaraeyat R, Mokhtari AR, Panahi P (2015) Chemical composition and antifungal activity of Trachyspermum copticum essential oil against Alternaria alternata (in vitro study). J Medicinal Plants 14 (53): 82–88. http://jmp.ir/article-1-971-en.htmL
Kowalska J, Tyburski J, Krzymińska J, Jakubowska M (2020) Cinnamon powder: an in vitro and in vivo evaluation of antifungal and plant growth promoting activity. Eur J Plant Pathol 156:237–243. https://doi.org/10.1007/s10658-019-01882-0
Liu J, Tian SP, Meng XH, Xu Y (2007) Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit. Postharvest Biol Technol 44:300–306. https://doi.org/10.1016/j.postharvbio.2006.12.019
Moraes SPCB, Bucker MW, Bucker MW, de Resende CG, Maciel KS, de Lima PAM, Ferreira A, Alexandre RS, Lopes JC (2018) Cinnamon and citronella essential oils in the in vitro control of the fungi Aspergillus sp. and Sclerotinia sclerotiorum. Afr J Agric Res 13(35):1811–1815. https://doi.org/10.5897/AJAR2018.13074
Nguyen VN, Seo DJ, Park RD, Jung WJ (2009) Antimycotic activities of cinnamon-derived compounds against Rhizoctonia solani in vitro. Biocontrol 54(5):697–707. https://doi.org/10.1007/s10526-009-9220-2
Ojaghian MR, Chen Y, Chen S, Cui ZQ, Xie GL, Zhang J (2014) Antifungal and enzymatic evaluation of plant crude extracts derived from cinnamon and rosemary against Sclerotinia carrot rot. Ann Appl Biol 164(3):415–429. https://doi.org/10.1111/aab.12111
Olea AF, Bravo A, Martínez R, Thomas M, Sedan C, Espinoza L, Zambrano E, Carvajal D, Silva-Moreno E, Carrasco H (2019) Antifungal activity of eugenol derivatives against Botrytis cinerea. Molecules 24:1239. https://doi.org/10.3390/molecules24071239
Rai M, Paralikar P, Jogee P, Agarkar G, Ingle AP, Derita M, Zacchino S (2017) Synergistic antimicrobial potential of essential oils in combination with nanoparticles: Emerging trends and future perspectives. Int J Pharm 519:67–78. https://doi.org/10.1016/j.ijpharm.2017.01.013
Ramezanian A, Azadi M, Mostowfizadeh-Ghalamfarsa R, Saharkhiz MJ (2016) Effect of Zataria multiflora Boiss and Thymus vulgaris L. essential oils on black rot of ‘Washington Navel’orange fruit. Postharvest Biol Technol 112:152–158. https://doi.org/10.1016/j.postharvbio.2015.10.011
Ranasinghe L, Jayawardena B, Abeywickrama K (2002) Fungicidal activity of essential oils of Cinnamomum zeylanicum (L..) and Syzygium aromaticum (L) Merr et LMPerry against crown rot and anthracnose pathogens isolated from banana. Lett Appl Microbiol 35(3):208–211. https://doi.org/10.1046/j.1472-765X.2002.01165.x
Sales MDC, Costa HB, Fernandes PMB, Ventura JA, Meira DD (2015) Antifungal activity of plant extracts with potential to control plant pathogens in pineapple. Asian Pac J Trop Biomed 6(1):26–31. https://doi.org/10.1016/j.apjtb.2015.09.026
Sarkhosh A, Schaffer B, Vargas AI, Palmateer AJ, Lopez P, Soleymani A (2018) In vitro evaluation of eight plant essential oils for controlling Colletotrichum, Botryosphaeria, Fusarium and Phytophthora fruit rots of avocado, mango and papaya. Plant Prot Sci 54: 153–162. https://doi.org/10.17221/49/2017-PPS
Shahriari D, Alibeiktehrani N, Maleki M (2017) The inhibitory effect of Thymus vulgaris and Carum copticum essential oil on the growth of Rhizoctonia solani, the causal agent of potato stem canker in the laboratory and greenhouse conditions. Appl Plant Protect 6(2):97–107
Shtienberg D, Elad Y, Niv A, Nitzani Y, Kirshner B (1998) Significance of leaf infection by Botrytis cinerea in stem rotting of tomatoes grown in non-heated greenhouses. Eur J Plant Pathol 104:753–763. https://doi.org/10.1023/A:1008690925443
Soylu EM, Yiğitbaş H, Tok FM, Soylu S, Kurt Ş, Baysal Ö, Kaya AD (2005) Chemical composition and antifungal activity of the essential oil of Artemisia annua L. against foliar and soil-borne fungal pathogens. Z Pflanzenk Flanzen 112:229–239. https://doi.org/10.1007/s11046-005-0206-z
Soylu EM, Soylu S, Kurt S (2006) Antimicrobial activities of the essential oils of various plants against tomato late blight disease agent Phytophthora infestans. Mycopathologia 161(2):119–128. https://doi.org/10.1007/s11046-005-0206-z
Soylu EM, Kurt S, Soylu S (2010) In vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mold disease agent Botrytis cinerea. Int J Food Microbiol 143:183–189. https://doi.org/10.1016/j.ijfoodmicro.2010.08.015
Steel RGD, Torrie JH (1980) Principles and procedures of statistic. McGraw Hill book Co Inc New York, USA
Vitali LA, Beghelli D, Biapa Nya PC, Bistoni O, Cappellacci L, Damiano S, Lupidi G, Maggi F, Orsomando G, Papa F, Petrelli D, Petrelli R, Quassinti L, Sorci L, Majd Zadeh M, Bramucci M (2016) Diverse biological effects of the essential oil from Iranian Trachyspermum ammi. Arab J Chem 9(6):775–786. https://doi.org/10.1016/j.arabjc.2015.06.002
Wang HW, Yang Z, Ying G, Yang M, Nian Y, Wei F, Kong W (2018) Antifungal evaluation of plant essential oils and their major components against toxigenic fungi. Ind Crop Prod 120:180–186. https://doi.org/10.1016/j.indcrop.2018.04.053
Wenneker M, Thomma BP (2020) Latent postharvest pathogens of pome fruit and their management: from single measures to a systems intervention approach. Eur J Plant Pathol 156(3):663–681. https://doi.org/10.1007/s10658-020-01935-9
Xie Y, Huang Q, Wang Z, Cao H, Zhang D (2017a) Structure-activity relationships of cinnamaldehyde and eugenol derivatives against plant pathogenic fungi. Ind Crop Prod 97:388–394. https://doi.org/10.1016/j.indcrop.2016.12.043
Xie Y, Wang Z, Huang Q, Zhang D (2017b) Antifungal activity of several essential oils and major components against wood-rot fungi. Ind Crop Prod 108:278–285. https://doi.org/10.1016/j.indcrop.2017.06.041
Yeole GJ, Teli NP, Kotkar HM, Mendki PS (2014) Cinnamomum zeylanicum extracts and their formulations control early blight of tomato. J Biopest 7(2):110
Zamanian Chaleshtori Z, Bonyadian M, Moshtaghi H, Ebrahimi A (2021) Antifungal effects of essential oils of Zataria multiflora, Mentha pulegium, and Mentha piperita. J Food Qual Hazards Control 8: 41–44. https://doi.org/10.18502/jfqhc.8.1.5462
Zarshenas MM, Moein M, Samani SM, Petramfar P (2014) An overview on ajwain (Trachyspermum ammi) pharmacological effects; modern and traditional. J Nat Med 14: 98–105. https://doi.org/10.18311/jnr/2014/96
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Ebrahimi, L., Jalali, H., Etebarian, H.R. et al. Evaluation of antifungal activity of some plant essential oils against tomato grey mould disease. J Plant Pathol 104, 641–650 (2022). https://doi.org/10.1007/s42161-022-01029-x
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DOI: https://doi.org/10.1007/s42161-022-01029-x