Abstract
Salinity and drought stress pose critical challenges to crop productivity, including roselle (Hibiscus sabdariffa L.). Using waste agriculture as a natural source of fertilizer to promote the activity of beneficial soil microorganisms has the potential to help agriculture in abiotic stress-affected areas by increasing plant nutrient uptake and ecological sustainability. We investigate the ability of BioSoy+ biofertilizer, which contains salt and drought stress-tolerant plant-growth-promoting rhizobacteria (PGPR) and soybean meal, to improve roselle growth under unfavorable conditions. Rhizobacteria tolerant to salt and drought stress were isolated, and evaluated for growth-promoting traits and pathogen inhibition under stress, and their identity confirmed by 16s rRNA gene sequencing. The impact of BioSoy+ on roselle growth and soil stability index during salt and drought stress was evaluated. Salt- and drought-tolerant PGPR strains Pseudomonas nicosulfuronedens AP01 and Bacillus velezensis CC03 were identified as the major component for biofertilizers. Under 2% NaCl stress, Pseudomonas nicosulfuronedens AP01 displayed outstanding phosphate solubilization and robust Sclerotium rolfsii pathogen suppression. BioSoy+ biofertilizer application significantly enhanced roselle growth under salt and water-limited conditions. BioSoy+ treatment, for example, boosted biomass by 194.74% and 68.29% at 25% field capacity and 100 mM NaCl conditions, respectively. BioSoy+ also increased relative water content, microbial activity, proline accumulation, and chlorophyll content, indicating stress reduction and better photosynthetic efficiency. This study highlights the importance of PGPR in alleviating the negative impacts of salt and drought stress. Furthermore, it emphasizes the feasibility of soybean meal as a biofertilizer carrier, fostering sustainable agricultural practices.
Graphical abstract
Highlights
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Pseudomonas nicosulfuronedens AP01 and Bacillus velezensis CC03 demonstrated plant-growth-promoting abilities under salt and drought stress conditions.
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BioSoy+, a PGPR-soybean meal biofertilizer, improved the stability index of soil.
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BioSoy+ application significantly increased overall plant health of roselle under salt and water-limitation conditions.
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BioSoy+ treatment reduced proline accumulation and enhanced chlorophyll content in roselle leaves, indicating stress alleviation and improved photosynthetic efficiency.
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Data Availability
Isolate CC03 and AP01 have been deposited in NCBI with the accession numbers OR304306.1 and OR304305.1, respectively. Pseudomonas nicosulfuronedens strain CC03 (16s rRNA, partial sequence) can be found at https://www.ncbi.nlm.nih.gov/nuccore/OR304306.1, and Bacillus velezensis strain AP01 (16s rRNA, partial sequence) at https://www.ncbi.nlm.nih.gov/nuccore/OR304305.1.
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Acknowledgements
We express our heartfelt gratitude to the Scholarship of Khon Kaen University (Thailand) for their generous financial support. This project was conducted as part of the Plant Genetic Project under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn at Khon Kaen University which was supported by the Fundamental Fund of Khon Kaen University and the National Science, Research and Innovation Fund (NSRF), Thailand under the Grant Number FRB650032/0161. Special thanks go to the Salt-tolerant Rice Research Group and the members of the Microbial Fertilizer Laboratory, Faculty of Science, Khon Kaen University, for their invaluable assistance and support in this endeavor.
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Riddech, N., Ma, Y.N. & Yodpet, B. Enhancing Growth of Roselle Plants (Hibiscus sabdariffa L.) Using a Salt- and Drought-Tolerant Rhizobacteria-Soybean Biofertilizer. Int J Environ Res 18, 26 (2024). https://doi.org/10.1007/s41742-024-00579-5
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DOI: https://doi.org/10.1007/s41742-024-00579-5