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Two-Dimension (2D) Cu-MOFs/aptamer Nanoprobe for In Situ ATP Imaging in Living Cells

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Abstract

Monitoring the intracellular adenosine triphosphate (ATP) level is vital for elaborating its function in physiological states. However, the intracellular fluorescence sensing of ATP using ATP aptamer remains difficult owing to non-target displacement and susceptibility toward enzymatic degradation. Herein, by combining 2D Cu-MOF nanosheets and FAM labelled ATP aptamer, we developed a fluorescent Cu-MOFs/aptamer nanoprobe to image and sense intracellular ATP. This nanoprobe reveals a very low fluorescence intensity because of the excellent quenching efficiency of 2D Cu-MOF nanosheets. The presence of ATP was capable to dissociate the FAM-aptamer from Cu-MOF nanosheets and resulted in an intense fluorescence signal. The Cu-MOFs/aptamer nanoprobe enables highly sensitive and selective measurement of ATP level ranging from 10 μM to 800 μM with a detection limit of 4.24 μM. This nanosystem also further realized in situ detection of the undulation of ATP trigged by drug stimulation, depending on the selective delivery of the nanoprobe and attractive capability of resisting nonspecific displacement. The constructed nanoprobe may supply a potential platform in clinical diagnostics and biological studies.

Graphical Abstract

Two-dimension Cu-MOFs nanosheets-aptamer nanoprobe was developed for in situ monitoring the change of ATP in living cells.

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Acknowledgements

This work was supported in part by the National Natural Science Foundation of China (21974125 and 21605038), the National 111 Project of China (D20003) and the Collaborative Innovation Project of Zhengzhou (Zhengzhou University) (Grant No. 18XTZX12002).

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Authors and Affiliations

  1. College of Chemistry, Green Catalysis Center, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou, 450001, China

    Xinxin Shi, Hui Xu, Yanan Wu, Yanmin Zhao, Hong-Min Meng, Zhaohui Li & Lingbo Qu

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  1. Xinxin Shi
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  2. Hui Xu
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  3. Yanan Wu
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  4. Yanmin Zhao
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  5. Hong-Min Meng
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  6. Zhaohui Li
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Correspondence to Hong-Min Meng or Zhaohui Li.

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Shi, X., Xu, H., Wu, Y. et al. Two-Dimension (2D) Cu-MOFs/aptamer Nanoprobe for In Situ ATP Imaging in Living Cells. J. Anal. Test. 5, 165–173 (2021). https://doi.org/10.1007/s41664-021-00172-1

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