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Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation

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Abstract

Objective

The present study aimed to evaluate root dentin collagen degradation after Nd:YAG laser irradiation by using polarized light microscopy and spectroscopy analysis.

Background

Quantification of collagen degradation is an important parameter to evaluate dentin caries progression.

Methods

Discs (2 mm in thickness × 6 mm in diameter) of bovine root dentin were obtained and submitted to demineralization by artificial caries lesion formation. The specimens were randomly divided into two groups according to the following treatments: C—control (no treatment) and L—Nd:YAG laser irradiation (1064 nm; 60 mJ; 10 Hz; 48 J/cm2; non-contact; 30 s). After the treatment, the specimens were exposed to the collagenase enzyme challenge for 5 days. Chemical analysis of the dentin surface was performed by FT-IR spectroscopy. Specimens were sectioned longitudinally to prepare slides for polarized light microscopy evaluation to provide the measure of depth of the degraded collagen in the demineralized area. Data were submitted to the Student’s t-test (p < 0.05).

Results

According to the t-Student test (t-value = 5.089), it was observed that the Nd:YAG laser showed statistically significant differences when compared to the control group (p < 0.001). Chemical differences in dentin were observed through the reduction of amide, carbonate, and phosphate groups present in the collagen matrix after laser irradiation.

Conclusions

Nd:YAG laser promoted chemical and morphological changes in the surface of the root dentin, showing effectiveness in reducing collagen degradation.

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Data Availability

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References

  1. Bowden GH, Ekstrand J, McNaughton B, Challacombe SJ (1990) The association of selected bacteria with the lesions of root surface caries. Oral Microbiol Immunol 5:346–351

    Article  Google Scholar 

  2. Kantola S (1972) Laser-induced effects on tooth structure IV: a study of changes in the calcium and phosphorus contents in dentine by electron probe microanalysis. Acta Odontol Scand 30:463–474

    Article  Google Scholar 

  3. Featherstone JDB, Barrett-Vespone NA, Fried D, Kantorowitz Z, Seka W (1998) CO2 laser inhibition of artificial caries-like lesion progression in dental enamel. J Dent Res 77:1397–1403

    Article  Google Scholar 

  4. Pereira DL, Freitas AZ, Bachmann L, Benetti C, Zezell DM, Ana PA (2018) Variation on molecular structure, crystallinity, and optical properties of dentin due to Nd:YAG laser and fluoride aimed at tooth erosion prevention. Int J Mol Sci 19(433):1–14

    Google Scholar 

  5. Myers TD, Myers WD, Stone RM (1989) First soft tissue study utilizing a pulsed Nd:YAG dental laser. Northwest Dent 68:14–17

    PubMed  Google Scholar 

  6. White JM, Goodis HE, Rose CL (1991) Use of the pulsed Nd:YAG laser for intraoral soft tissue surgery. Lasers Surg Med 11:455–461

    Article  Google Scholar 

  7. Hess JA (1990) Scanning electron microscopic study of laser-induced morphologic changes of a coated enamel surface. Lasers Surg Med 10:458–462

    Article  Google Scholar 

  8. Gutknecht N, Moritz A, Dercks HW, Lampert F (1997) Treatment of hypersensitive teeth using neodymium:yttrium-aluminum- garnet lasers: a comparison of the use of various settings in an in vivo study. J Clin Laser Med Surg 15:171–174

    Article  Google Scholar 

  9. Schaller HG, Weihing T, Strub JR (1997) Permeability of dentine after Nd:YAG laser treatment: an in vitro study. J Oral Rehabil 24(4):274–281

    Article  Google Scholar 

  10. Goodis HE, White JM, Marshall GW et al (1997) Effects of Nd: and Ho:yttrium-aluminium-garnet lasers on human dentine fluid flow and dental pulp-chamber temperature in vitro. Arch Oral Biol 42:845–854

    Article  Google Scholar 

  11. Pashley EL, Himer JA, Liu M, Kim S, Pashley DH (1992) Effects of CO 2 laser energy on dentin permeability. J Endod 18(6):257–262

    Article  Google Scholar 

  12. White JM, Goodis HE, Setcos JC, Eakle S, Hulscher BE, Rose CL (1993) Effects of pulsed Nd:YAG laser energy on human teeth: a three-year follow-up study. J Am Dent Assoc 124:45–51

    Article  Google Scholar 

  13. Gonçalves SEP, de Araujo MA, Damião AJ (1999) Dentin bond strength: influence of laser irradiation, acid etching, and hypermineralization. J Clin Laser Med Surg 17(2):77–85

    Article  Google Scholar 

  14. Zezell DM, Boari HGD, Ana PA, de Eduardo CP, Powell GL (2009) Nd:YAG laser in caries prevention: a clinical trial. Lasers Surg Med 41:31–35

    Article  Google Scholar 

  15. Bertassoni LE, Habelitz S, Kinney JH, Marshall SJ Jr, Marshall GW (2009) Biomechanical perspective on the remineralization of dentin. Caries Res 43:70–77

    Article  Google Scholar 

  16. Islam MS, Khunkar SJ, Nakashima S, Sadr A, Nikaido T, Tagami J (2016) Comparative study of demineralized collagen degradation determined by hydroxyproline assay and microscopic depth measurement. J Dent 47:94–97

    Article  Google Scholar 

  17. Cortes D, Ellwood RP, Ekstrand KR (2003) An in vitro comparison of a combined FOTI/visual examination of occlusal caries with other caries diagnostic methods and the effect of stain on their diagnostic performance. Caries Res 37(1):8–16

    Article  Google Scholar 

  18. Arnold WH, Gaengler P (2007) Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth. Ann Anat 189:183–190

    Article  Google Scholar 

  19. Maske TT, Isolan CP, van de Sande FH et al (2015) A biofilm cariogenic challenge model for dentin demineralization and dentin bonding analysis. Clin Oral Investig 19(5):1047–1053

    Article  Google Scholar 

  20. Anido-Anido A, Amore R, Lewgoy HR, Anauate-Netto C, Silva TM, Paiva Gonçalves SE (2012) Comparative study of the bond strength to human and bovine dentin in three different depths. Braz Dent Sci 15(2):56–62

    Article  Google Scholar 

  21. Queiroz CS, Hara AT, Paes Leme AF, Cury JA (2008) pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization. Braz Dent J 19:21–27

    Article  Google Scholar 

  22. Silva TM, Gonçalves LL, Fonseca BM et al (2016) Influence of Nd:YAG laser on intrapulpal temperature and bond strength of human dentin under simulated pulpal pressure. Lasers Med Sci 31(1):49–56

    Article  Google Scholar 

  23. Kato MT, Leite AL, Hannas AR et al (2012) Impact of protease inhibitors on dentin matrix degradation by collagenase. J Dent Res 91:1119–1123

    Article  Google Scholar 

  24. Feitosa FA, Crastechini E, Esteves SRMS, Silva TM, Huhtala MFRL, Gonçalves SEP (2017) Conventional and experimental treatments for pit and fissure sealing associated with ND:YAG laser. Lasers Dent Sci 1(1):33–40

    Article  Google Scholar 

  25. dos Santos PM, Cardoso MAG, Khouri S, de Paula Júnior AR, Uehara M, Sakane KK (2012) Utilização da microespectroscopia infravermelha (FT-IR) para teste de algoritmos estatísticos na diferenciação dos micro-organismos Candida albicans, Candida dubliniensis e Candida parapsilosis. Rev Bras Eng Biomed 28(4):398–409

    Article  Google Scholar 

  26. Roche D, Ségalen L, Balan E, Delattre S (2010) Preservation assessment of Miocene-Pliocene tooth enamel from Tugen Hills (Kenyan Rift Valley) through FTIR, chemical and stable-isotope analyses. J Archaeol Sci 37(7):1690–1699

    Article  Google Scholar 

  27. Nakamichi I, Iwaku M, Fusayama T (1983) Bovine teeth as possible substitutes in the adhesion test. J Dent Res 62:1076–1081

    Article  Google Scholar 

  28. Schilke R, Lisson JA, Bauß O, Geurtsen W (2000) Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation. Arch Oral Biol 45:355–361

    Article  Google Scholar 

  29. Hara AT, Queiroz CS, Paes Leme AF, Serra MC, Cury JA (2003) Caries progression and inhibition in human and bovine root dentine in situ. Caries Res 37:339–344

    Article  Google Scholar 

  30. Celiberti P, Carvalho TS, Raggio DP, Mendes FM (2012) Influence of dental materials used for sealing caries lesions on laser fluorescence measurements. Lasers Med Sci 27:287–295

    Article  Google Scholar 

  31. de Magalhães MF, Matson E, de Rossi W, Alves JB (2004) A morphological in vitro study of the effects of Nd:YAG laser on irradiated cervical dentin. Photomed Laser Surg 22:527–532

    Article  Google Scholar 

  32. Supuran CT, Scozzafava A, Clare BW (2002) Bacterial protease inhibitors. Med Res Rev 22:329–372

    Article  Google Scholar 

  33. Magalhães AC, Rios D, Machado MADAM et al (2008) Effect of Nd:YAG irradiation and fluoride application on dentine resistance to erosion in vitro. Photomed Laser Surg 26:559–563

    Article  Google Scholar 

  34. Hsu PJ, Chen JH, Chuang FH, Roan RT (2006) The combined occluding effects of fluoride-containing dentin desensitizer and Nd-YAG laser irradiation on human dentinal tubules: an in vitro study. Kaohsiung J Med Sci 22:24–29

    Article  Google Scholar 

  35. Kara C, Orbak R (2009) Comparative evaluation of Nd:YAG laser and fluoride varnish for the treatment of dentinal hypersensitivity. J Endod 35:971–974

    Article  Google Scholar 

  36. Yazıcı E, Gurgan S, Gutknecht N, Imazato S (2010) Effects of erbium: yttrium-aluminum-garnet and neodymium: yttrium-aluminum-garnet laser hypersensitivity treatment parameters on the bond strength of self-etch adhesives. Lasers Med Sci 25:511–516

    Article  Google Scholar 

  37. Esteves SRMS, Huhtala MFRL, Gomes APM, Ye Q, Spencer P, de Paiva Gonçalves SE (2016) Longitudinal effect of surface treatments modified by NaOCl-induced deproteinization and Nd:YAG laser on dentin permeability. Photomed Laser Surg 34:68–75

    Article  Google Scholar 

  38. Khosa AA, Xu T, Xia BQ, Yan J, Zhao CY (2019) Technological challenges and industrial applications of CaCO3/CaO based thermal energy storage system – a review. Sol Energy 193(15):618–636

    Article  Google Scholar 

  39. Van Meerbeek B, Yoshihara K, Van Landuyt K, Yoshida Y, Peumans M (2020) From Buonocore’s pioneering acid-etch technique to self-adhering restoratives. A status perspective of rapidly advancing dental adhesive technology. J Adhes Dent 22:7–34

    PubMed  Google Scholar 

  40. He H, Yu J, Song Y, Lu S, Liu H, Liu L (2009) Thermal and morphological effects of the pulsed Nd:YAG laser on root canal surfaces. Photomed Laser Surg 27:235–240

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Restorative Dentistry, Institute of Science and Technology of São José dos Campos, Sao Paulo State University (UNESP), Av. Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, 12245-000, São Paulo, Brazil

    Stephanie Ribeiro Lopes, Andrea Maselli & Sérgio Eduardo de Paiva Gonçalves

  2. Department of Restorative Dentistry, Anhanguera University of São Paulo, São José dos Campos, São Paulo, Brazil

    Tânia Mara da Silva & Lucélia Lemes Gonçalves

  3. Department of Physics, Technological Institute of Aeronautics (ITA), São José dos Campos, São Paulo, Brazil

    Tiago Moreira Bastos Campos

Authors
  1. Stephanie Ribeiro Lopes
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  2. Tânia Mara da Silva
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  3. Andrea Maselli
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  4. Lucélia Lemes Gonçalves
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  5. Tiago Moreira Bastos Campos
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  6. Sérgio Eduardo de Paiva Gonçalves
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Stephanie Ribeiro Lopes, Andrea Maselli, Tânia Mara da Silva, Lucélia Lemes Gonçalves, Tiago Moreira Bastos Campos, and Sérgio Eduardo de Paiva Gonçalves. The first draft of the manuscript was written by Stephanie Ribeiro Lopes and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sérgio Eduardo de Paiva Gonçalves.

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Lopes, S., da Silva, T., Maselli, A. et al. Chemical and morphological analysis of dentin collagen degradation after Nd:YAG laser irradiation. Laser Dent Sci 6, 47–53 (2022). https://doi.org/10.1007/s41547-022-00149-y

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  • DOI: https://doi.org/10.1007/s41547-022-00149-y

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