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Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes

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Abstract

Potato (Solanum tuberosum L.) is considered as one of the most important staple foods in the world. Classical breeding for resistance to pests, pathogens and viruses involves the identification of resistance sources, which are often found in different wild potato species. Potato virus X (PVX) attacks potato worldwide, causing considerable amount of yield losses, reaching 20% of productivity depending upon the virus strain. To decrease its impact, there’s serious interest to develop new varieties carrying the Rx1 and/or Rx2 genes which originally came from the wild species Solanum tuberosum ssp. andigena and Solanum acaule, respectively; these genes are able to provide extreme resistance to PVX which could be facilitated with molecular marker-assisted selection. In this study, twenty-five potato genotypes, belong to Potato Research Center, were tested to detect the presence of Rx1 and Rx2 extreme resistance genes of PVX by using 5Rx1 and 106Rx2 specific markers, respectively, to enhance breeding efficiency for the purpose of developing new varieties. The results showed that three genotypes carried the 5Rx1 marker. On the other hand, the results with the 106Rx2 marker showed that thirteen individuals (52%) of the genotypes carried the 106Rx2 marker. Sixteen genotypes (64%) are resistant to PVX and have a great potential for gene introgression, while the other nine genotypes were not carriers of the markers. Genotypes those are carrying the markers were strongly recommending to be used by breeders to develop new PVX extreme resistance potato varieties.

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Acknowledgements

This work was funded by the Government of Hungary and FAO together. Authors are grateful for generosity help of Hungarian Government and the FAO to accomplish the study; they also thank the Society of University of Pannonia, Georgikon Faculty for hosting.

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Authors and Affiliations

  1. Botany and Microbiology Department, Science College, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia

    Hassan O. Shaikhaldein & Ibrahim A. Alaraidh

  2. Plant Sciences and Biotechnology Department, Pannonia University , Georgikon Faculty 16. Deák Ferenc str., Keszthely, 8360, Hungary

    Borbala Hoffmann

  3. Plant Protection and Biomolecular Diagnosis Department, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications, Alexandria, 21934, Egypt

    Dalia G. Aseel

Authors
  1. Hassan O. Shaikhaldein
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  2. Borbala Hoffmann
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  3. Ibrahim A. Alaraidh
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  4. Dalia G. Aseel
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Correspondence to Dalia G. Aseel.

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Shaikhaldein, H.O., Hoffmann, B., Alaraidh, I.A. et al. Evaluation of extreme resistance genes of Potato virus X (Rx1 and Rx2) in different potato genotypes. J Plant Dis Prot 125, 251–257 (2018). https://doi.org/10.1007/s41348-018-0148-6

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  • DOI: https://doi.org/10.1007/s41348-018-0148-6

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