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No association of CLOCK 3111T/C polymorphism with diurnal preference and sleep quality in Korean adults

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Abstract

The CLOCK 3111T/C polymorphism has been associated with evening preference, but most of the following studies did not replicate the original finding. Although several studies suggested that the sleep quality was also influenced by genetic factors, the relationship of CLOCK 3111T/C polymorphism with sleep quality has not been identified in general population. We aimed to examine whether there is a difference in CLOCK 3111T/C polymorphism according to the morningness–eveningness in Korean adults, and to find a difference in sleep quality according to this polymorphism. Forty-five morning type (MT) (age: 43.93 ± 12.51 years, M:F = 14:31), 59 neither type (NT) (age: 35.20 ± 9.53 years, M:F = 20:39), and 52 evening type (ET) (age: 29.08 ± 7.94 years, M:F = 15:37) subjects were finally selected after excluding those with sleep disorders or shift work. The CLOCK 3111T/C was analyzed by DNA sequencing or SNaPshot assay. The genotype distribution, C allele frequency, and proportion of C allele positive subjects were significantly different between the MT, NT and ET groups. There was no significant difference in PSQI scores between the subjects with TC genotype and age-, sex- and ME type-matched subjects with TT genotype (n = 15 for each group). It is difficult to say that our result replicates the previous finding that showed an association of CLOCK 3111C allele with evening preference, for there was no subject with CLOCK 3111CC homozygote in our study. Moreover, the hypothesis that CLOCK 3111T/C polymorphism may be associated with sleep quality was not supported by our study.

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Acknowledgments

The authors would like to thank Dr. Duffy JF (Harvard Medical School) for valuable comments on subject recruitment and statistical analysis of the present study. We are grateful to the staff of Chunchon National Museum of Art for their assistance in recruiting our subjects.

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Authors and Affiliations

  1. Department of Psychiatry, Yesarang Hospital, Cheongju, South Korea

    Seong Jae Kim

  2. Department of Psychiatry, Kangwon National University Hospital, Chunchon, South Korea

    Jung Hie Lee, Se Yong Lee & Jun-Won Hwang

  3. Division of Sleep and Circadian Disorders, Department of Medicine, Brigham & Women’s Hospital, Harvard Medical School, Boston, MA, USA

    Jung Hie Lee

  4. Department of Laboratory Medicine, Kangwon National University Hospital, Chunchon, South Korea

    In Bum Suh

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  1. Seong Jae Kim
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Correspondence to Jung Hie Lee.

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The authors report no financial conflicts of interest associated with the present article. This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0003160 to JHL).

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Kim, S.J., Lee, J.H., Lee, S.Y. et al. No association of CLOCK 3111T/C polymorphism with diurnal preference and sleep quality in Korean adults. Sleep Biol. Rhythms 14, 135–140 (2016). https://doi.org/10.1007/s41105-015-0033-7

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  • DOI: https://doi.org/10.1007/s41105-015-0033-7

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