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Characterization of Geobacillus stearothermophilus and its application in soil stabilization

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Abstract

Microorganism involvement in carbonate precipitation reduces the CO2 emissions seen in construction engineering. Microbial induced calcium precipitation with ureolytic bacteria (UB) is an environmental friendly technique that finds use in the stabilisation of sand and seal cracks. In the present study, soil samples were collected from a marine fishery harbour. A total of 56 isolates were obtained from four soil samples. 18 isolates (UB1–UB18) considered phenotypic variant were subjected to primary and secondary screening. Isolate UB13 showing the highest urease activity was selected for optimization of parameters for small scale biocementation. UB13 was determined as Geobacillus stearothermophilus by 16S rRNA sequencing. Different parameters such as calcium chloride molarity, urea concentration, inoculum size and reaction time were used in this study. The specific urease activity of G. stearothermophilus was unaffected by high urea concentration. The highest calcium carbonate yield of 640 mg was obtained with 8% urea concentration. The UCS test revealed B4 with varied urea concentration having highest compressive strength. The calcite content recovered after UCS test also showed B4 as the best bacterial growth condition for biomineralization.

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Authors and Affiliations

  1. Department of Civil Engineering, Aarupadai Veedu Institute of Technology Deemed to Be Vinayaka Missions Research Foundation, Chennai, 603104, India

    Pa. Suriya & S. P. Sangeetha

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  1. Pa. Suriya
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  2. S. P. Sangeetha
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Correspondence to Pa. Suriya.

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Pa. Suriya (Research Scholar) conducted all the experiments and wrote the manuscript. Dr. S. P. Sangeetha (Professor) revised the manuscript.

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Suriya, P., Sangeetha, S.P. Characterization of Geobacillus stearothermophilus and its application in soil stabilization. J Build Rehabil 7, 101 (2022). https://doi.org/10.1007/s41024-022-00244-4

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  • DOI: https://doi.org/10.1007/s41024-022-00244-4

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