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Calcium-Activated Potassium Channel (KCNMA1) as Biomarker of Pre-Invasive and Invasive Cervical Cancer

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Indian Journal of Gynecologic Oncology Aims and scope Submit manuscript

Abstract

Purpose of the Study

Several ion channels including calcium-activated potassium channels like KCNMA1 have been proposed as tumor markers and therapeutic targets for various cancers. KCNMA1 channel expression has been found to be progressively increasing with increasing severity of the lesion in samples collected retrospectively. Therefore, we aimed to prospectively study the mRNA and protein expression of KCNMA1 in pre-invasive and invasive cervical cancer.

Methods

Sixty women were recruited to the study and allocated equally (n = 15) into four groups on the basis of histopathology, i.e., control (Group 1), cervical intraepithelial neoplasia CIN1 (Group 2), CIN2/CIN3 (Group 3) and invasive cervical carcinoma (Group 4). Real-time PCR and immunohistochemistry were used for assessing mRNA and protein expression of KCNMA1 at mRNA and protein level, respectively.

Results

The mean KCNMA1 mRNA levels in Groups 1, 2, 3 and 4 were 0.23 (SD ± 0.58), 271.40 (SD ± 1050.21), 298.84 (SD ± 1153.33) and 326.54 (SD ± 861.97), respectively (p = 0.039). Protein expression was positive in 34% in CIN1, 80% in CIN2/CIN3 and 100% in the cervical cancer group (p = < 0.001). On subgroup analysis in cervical cancer, KCNMA1 mRNA and protein expressions were higher in tumor size > 4 cm, poorly differentiated tumors, deep stromal invasion and non-keratinizing squamous cell carcinoma.

Conclusions

KCNMA1 protein and mRNA expressions were significantly different between the groups studied. The expression was found to increase with severity of lesion. To the best of our knowledge, this is the first prospective study on KCNMA1 channels in cervical pre-cancer and cancer. Further exploratory studies can be done to establish KCNMA1 as a prognostic biomarker.

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Funding

The authors declare that no funds, grants or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. ESIC Medical College & Hospital, Bihta, Patna, Bihar, India

    Puja Kumari

  2. Department of Obstetrics and Gynaecology, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

    Nazia Parveen, Bindiya Gupta, Sandhya Jain & Madeeha Mudassir

  3. Department of Obstetrics and Gynaecology, AIIMS Rishikesh, Rishikesh, India

    Shalini Rajaram

  4. Department of Biochemistry, University College of Medical Sciences and Guru Teg Bahadur Hospital, New Delhi, India

    Rajarshi Kar

  5. Department of Pathology, All India Institute of Medical Sciences (Guwahati), Guwahati, Assam, India

    Priyanka Gogoi

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  1. Puja Kumari
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Contributions

Kumari P and Parveen N were responsible for protocol/project development, data collection or management, data analysis and manuscript writing/editing. Gupta B was involved in protocol/project development, data collection or management, data analysis, manuscript writing/editing and critical revision of manuscript. Rajaram S and Sandhya Jain contributed to protocol/project development, manuscript writing/editing and critical revision of manuscript. Kar R took part in data collection or management, data analysis and critical revision of manuscript. Gogoi P participated in data collection or management, data analysis and manuscript writing/editing. Mudassir M assisted with data analysis, manuscript writing/editing and critical revision of manuscript.

Corresponding author

Correspondence to Bindiya Gupta.

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Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

Ethical approval was taken from the Institutional Ethics Committee—Human Research (IEC-HR), University College of Medical Science, University of Delhi, Delhi 95 (Letter No. IEC-HR/2018/36/85R).

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Informed consent was obtained from all individual participants included in the study.

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Kumari, P., Parveen, N., Gupta, B. et al. Calcium-Activated Potassium Channel (KCNMA1) as Biomarker of Pre-Invasive and Invasive Cervical Cancer. Indian J Gynecol Oncolog 22, 40 (2024). https://doi.org/10.1007/s40944-024-00822-z

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  • DOI: https://doi.org/10.1007/s40944-024-00822-z

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