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Long noncoding RNA HOTAIR as a biomarker for the detection of Cervical Cancer and Cervical Intraepithelial Neoplasia

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Indian Journal of Gynecologic Oncology Aims and scope Submit manuscript

Abstract

Purpose

This study aimed to investigate the clinical significance of HOX transcript antisense RNA (HOTAIR) expression as a biomarker for the detection of cervical cancer and cervical premalignant lesions.

Patients & Methods

41 cervical cancer biopsies, 50 precancerous lesions, and 40 normal adjacent tissues were collected. A Series of qPCR analysis was performed on cervical cancer cells, and biopsy specimens to measure the expression of HOTAIR. We assessed the diagnostic value of HOTAIR expression levels and its correlation with clinical and pathological criteria.

Results

The expression levels of HOTAIR in tumor tissues, premalignant lesions, and adjacent normal tissues showed a significant difference (P < 0.0001). HOTAIR levels were higher in tumors compared to controls, and to premalignant lesions. Simultaneously, HOTAIR levels were significantly elevated in premalignant lesions versus controls. HOTAIR overexpression was also associated with progressed FIGO stages (P < 0.0001), tumor size (P < 0.0001), lymph node metastasis (P < 0.0001), and distant metastasis (P < 0.0001). For the diagnostic value, our results showed that HOTAIR could discriminate patients with cervical cancer from controls with 85,37% sensitivity and 85,00% specificity (AUC = 0,9305). In addition, HOTAIR expression levels could also discriminate cervical intraepithelial neoplasia from controls with 96% sensitivity and 65% specificity (AUC = 0,7835).

Conclusion

Our findings suggest that HOTAIR might constitute a potential biomarker for detecting cervical cancer at premalignant and malignant stages as well as distinguishing key clinicopathological features.

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Acknowledgements

This work has been supported by Moroccan Ministry of Higher Education, the University Hassan II of Casablanca-Faculty of Sciences and Techniques of Mohammedia. The mobility of the author Maryame Lamsisi for fellowship at Laboratory of Infectious Agents and Hygiene GIMAP-EA3064, University Hospital & University Jean Monnet, Saint-Etienne, France has been financed as part of the APUR19 project (Appel a projet universitaire de recherche 2019). Authors also would like to thank the Team members of Virology, Oncology and Medical Biotechnology, and Virology Microbiology, Quality and Biotechnologies / Ecotoxicology and Biodiversity laboratory Hassan II University of Casablanca, Faculty of science and techniques, for all their efforts to support us during this study.

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Author information

Authors and Affiliations

  1. Laboratory of Virology, Microbiology, Quality, and Biotechnologies/ ETB. Faculty of Science and Techniques Mohammedia, Hassan II University of Casablanca, BP 146 20650, Mohammedia, Morocco

    Maryame Lamsisi, Mustapha Benhessou, Amal Bouziyane, Youssef Ennaji, Berjas Abumsimir & Moulay Mustapha Ennaji

  2. Laboratory of Infectious Agents and Hygiene GIMAP-EA3064, University Hospital & University Jean Monnet, Saint-Etienne, France

    Guorong Li & Thomas Bourlet

  3. Mohammed VI Centre for Treatment of Cancer, University Hospital Ibn Rochd, Casablanca, Morocco

    Mustapha Benhessou, Mohamed El Karroumi & Imane Joudar

  4. Biology and Medical Research Unit, CNESTEN, Rabat, Morocco

    Mohammed El Mzibri

  5. School of Medicine and Pharmacy, University Mohammed VI of Health Science, Casablanca, Morocco

    Amal Bouziyane

  6. Laboratory of Virology, Pasteur Institute, Casablanca, Morocco

    Lahcen Wakrime

  7. Research and Biosafety Laboratory, Mohammed V Military Instruction Hospital, University Mohammed V of Rabat, Rabat, Morocco

    Abdelilah Laraqui

Authors
  1. Maryame Lamsisi
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  2. Guorong Li
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  13. Moulay Mustapha Ennaji
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Contributions

ML: Conceptualization, Methodology, Formal analysis and investigation, Data analysis, Writing—original draft preparation; GL: Methodology, Writing—review and editing; MB: Providing resources, Formal analysis and investigation; MEM: Conceptualization, Providing resources; AB: Methodology, providing resources, Writing—review and editing; LW: Providing resources, Methodology; AL: Providing resources; YE: Formal analysis and investigation; BA: Conceptualization, Data Analysis, Writing—review and editing; MEK: Providing resources; IJ: Formal analysis and investigation; TB: Providing resources, Supervision; MME: Conceptualization, Supervision.

Corresponding author

Correspondence to Moulay Mustapha Ennaji.

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Conflict of interest

The authors declare no conflict of interest.

Ethics Approval

The study was approved by the Biomedical Research Ethics Committee of the School of Medicine and Pharmacy of Casablanca.

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Lamsisi, M., Li, G., Benhessou, M. et al. Long noncoding RNA HOTAIR as a biomarker for the detection of Cervical Cancer and Cervical Intraepithelial Neoplasia. Indian J Gynecol Oncolog 19, 69 (2021). https://doi.org/10.1007/s40944-021-00552-6

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  • DOI: https://doi.org/10.1007/s40944-021-00552-6

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