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Survival niches of Pseudomonas savastanoi pv. glycinea, causal agent of bacterial blight in soybean (Glycine max L.)

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Abstract

Bacterial blight caused by Pseudomonas savastanoi pv. glycinea (Psg) is a prevalent disease in soybean crops worldwide, and crop rotation is a potential management strategy. However, the importance of alternative crops and soil as hosts of Psg is not well understood. This study aimed to assess the survival of Psg in four soil types, the phyllosphere, and rhizosphere of 12 crops rotated with soybean, and endophytic colonization of crops by Psg. The pathogenic strain Soj. 1462 of Psg, resistant to rifampicin, was used in all experiments, and culturing followed by PCR confirmed bacterial survival. Psg survived for a maximum of two days in all soil types. In both survival niches, phyllosphere and rhizosphere, sunn hemp, common bean, and turnip proved to be the crops with a shorter Psg survival period, lasting less than 28 days, while the remaining evaluated crops exhibited longer survival periods. In endophytic colonization, Psg survived for less than 35 days in these crops when inoculated in artificially wounded plants. When inoculated by spray in the same crops, the highest Psg survival was recorded up to 28 days. To reduce Psg inoculum, it is advisable to cultivate sunn hemp, common bean, and turnip in succession with soybean, given their comparatively lower recorded survival periods.

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Data availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

This study was partially funded by the Coordination for the Improvement of Higher Education Personnel – Brazil (CAPES) – Financial Code 001.

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Authors and Affiliations

  1. Departamento de Proteção Vegetal, Faculdade de Ciências Agronômicas (FCA), Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Avenida Universitária, 3780, Botucatu, SP, 18610-034, Brazil

    Luana Laurindo de Melo, Daniele Maria do Nascimento, José Marcelo Soman, João César Lourencetti da Silva & Antonio Carlos Maringoni

  2. Departamento de Produção e Melhoramento Vegetal, FCA/UNESP, São Paulo, Brazil

    Maria Márcia Pereira Sartori

  3. Departamento de Ciências da Produção Agrícola, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista (Unesp), Jaboticabal, SP, Brazil

    Tadeu Antônio Fernandes da Silva Júnior

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  1. Luana Laurindo de Melo
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  2. Daniele Maria do Nascimento
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  3. José Marcelo Soman
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  4. João César Lourencetti da Silva
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  5. Maria Márcia Pereira Sartori
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  7. Tadeu Antônio Fernandes da Silva Júnior
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by LLM, DMN, JMS, JCLS, MMPS, ACM and TAFSJ. The first draft of the manuscript was written by LLM and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Luana Laurindo de Melo.

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de Melo, L.L., do Nascimento, D.M., Soman, J.M. et al. Survival niches of Pseudomonas savastanoi pv. glycinea, causal agent of bacterial blight in soybean (Glycine max L.). Trop. plant pathol. 49, 436–448 (2024). https://doi.org/10.1007/s40858-024-00642-8

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