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Assessment of vırulence patterns and pathogenıcıty-related genes of Colletotrichum falcatum Went in Bangladesh

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Abstract

Sugarcane (Saccharum officinarum L.) is one of the important cash crops and the main source of white sugar in tropical and sub-tropical regions. Colletotrichum falcatum Went is the causative agent of sugarcane red rot disease worldwide. This pathogen is diverse in pathological and molecular characteristics. This study is aimed to assess the vırulence patterns and role of pathogenıcity-related genes of C. falcatum Went. A total of 41 isolates were isolated from four sugarcane major growing regions of Bangladesh from 2018 to 2019 cropping seasons. The virulence test field experiment was conducted at Bangladesh Sugarcrop Research Institute (BSRI) research farm in randomized completely block design (RCBD) with three replications from January to December in 2019 and 2020. The pathogenicity-related gene molecular experiments were carried out at the Bacteriology Laboratory, University Putra Malaysia (UPM). The assessment of disease parameters was based on the published procedures. Out of 41 isolates, three isolates were virulent,18 isolates were moderately virulent and 13 isolates were the least virulent. The rest of the seven isolates had demonstrated different virulence attributes in the assessment periods. All C. falcatum isolates possessed PKS1, HXT1, and Pel2 genes and had a high similarity value but showed variation among the virulent, moderately virulent, and the least virulent isolates. Nucleotide substitutions and deletions in some loci are the possible causes of variation among them.These findings would help in understanding the variability of C. falcatum isolates based on sugarcane differential hosts, and the role of pathogenicity-related genes during pathogenesis.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank the staff from the Bangladesh Sugarcrop Research Institute (BSRI) and Department of Plant Protection, Universiti Putra Malaysia, for their excellent technical assistance during the research.

Funding

The National Agricultural Technology Program (NATP Phase II) Project of the Bangladesh Agricultural Research Council (BARC) provided financial support with grant number: 6282513.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Md Imam Hossain, Ganesan Vadamalai, Norsazilawati Saad, Muhammad Ziaur Rahman, Abdulaziz Bashir Kutawa, Syazwan Afif Bin Mohd Zobir & Khairulmazmi Ahmad

  2. Pathology Division, Bangladesh Sugarcrop Research Institute (BSRI), Ishurdi, 6620, Pabna, Bangladesh

    Md Imam Hossain & Md Omar Khaiyam

  3. Laboratory of Sustainable Agronomy and Crop Protection, Institute of Plantation Studies, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Erneeza Mohd Hata & Khairulmazmi Ahmad

  4. Institute of Tropical Agriculture and Food Security, University Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Khairulmazmi Ahmad

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  1. Md Imam Hossain
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  2. Ganesan Vadamalai
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Contributions

MIH and KA conceived the study. MIH gathered sequences to constitute the final dataset. MIH performed the analyses and interpreted the data with the help of GV. NS, EMH, MZR, and ABK wrote the manuscript with contributions from the co-authors.

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Correspondence to Khairulmazmi Ahmad.

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Hossain, M.I., Vadamalai, G., Saad, N. et al. Assessment of vırulence patterns and pathogenıcıty-related genes of Colletotrichum falcatum Went in Bangladesh. Trop. plant pathol. 47, 737–753 (2022). https://doi.org/10.1007/s40858-022-00531-y

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