Abstract
Frogeye leaf spot (FLS) of soybean caused by Cercospora sojina Hara is an important disease in the Argentine Pampas region. The primary inoculum sources of the disease are infected seeds and plant debris. Thus, use of pathogen-free or fungicide-treated seeds is crucial to prevent the introduction and further spread of the disease. The aims of this research were i) to quantify C. sojina incidence in harvested soybean seeds, ii) to compare media-based incubation methods for detection of the pathogen in seeds, and iii) to evaluate the effect of fungicide seed treatments in reducing FLS incidence. Among eight different detection methods evaluated, no difference in pathogen incidence was found, but the water restriction blotter method should be recommended as it is more time and cost-effective. Out of 13 different fungicide seed treatment combinations in three independent experiments, benzimidazole seed treatments resulted in zero FLS incidence. Any other of the tested fungicidal active ingredients either did not control the disease or did so poorly only when initial infection levels were low. Treating seeds with benzimidazole fungicides may help to reduce FLS incidence during initial soybean field stands when using infected seed lots.
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References
Bisht VS, Sinclair JB (1985) Effect of Cercospora sojina and Phomopsis sojae alone or in combination on seed quality and yield of soybeans. Plant Disease 69:436–439
Carmona MA, Scandiani MM, Luque A (2009) Severe outbreaks of soybean frogeye leaf spot in the Pampean region. Argentina. Plant Disease 93:966
Carmona M (2011) Damages caused by frogeye leaf spot and late season disease in soybean in Argentina and control criteria. Tropical Plant Pathology 36:1356–1358
Carmona M, Sautua F, Reis E (2015) Soybean frogeye leaf spot (Cercospora sojina): first economic damage threshold determination. Advances in Applied Agricultural Science 3:1–7
Davidse LC, Ishii H (1995). Biochemical and molecular aspects of the mechanisms of action fo benzimidazoles, N-phenylcarbamates and N-phenylformamidoximes and the mechanisms of resistance to these compounds in fungi. In: Lyr H (Ed.) Modern Selective Fungicides: Properties, Applications, Mechanisms of Action. 2nd Ed. Villengang, Germany. Spektrum Akademischer Verlag. pp. 306–322
Fox J, Weisberg S (2011) An R companion to applied regression, second edition. Sage, Thousand oaks
Leach C (1963) The qualitative and quantitative relationship of monochromatic radiation to sexual and asexual reproduction of Pleospora herbarum. Mycologia 55:151–163
Lehman SG (1934) Frogeye (Cercospora diazu Miura) on stems, pods and seeds of soybean and the relation of these infections to recurrence of the disease. Journal of Agricultural Research 48:131–147
Machado JC, Langerak CJ, Jaccoud-Filho DS (eds.) (2002) Seed-borne fungi: a contribution to routine seed health analysis. 1st Ed. Lavras, Brazil. International Seed Testing Association (ISTA)
Machado JC, Da Cruz De Oliveira JÁ, Vieira M, Das G, Alves M (2003) Controle da germinação de sementes de soja em testes de sanidade pelo uso da restrição hídrica. Revista Brasileira de Sementes 25:77–81
Mathur SB, Kongsdal O (eds.) (2003) Common laboratory seed health testing methods for detecting fungi. 1st Ed. Copenhagen, Denmark; Switzerland. International Seed Testing Association (ISTA)
Mwase WF, Kapooria RG (2000) Incidence and severity of frogeye leaf spot and associated yield losses in soybeans in agroecological zone II of Zambia. Mycopathologia 149:73–78
Phillips DV (1999) Frogeye leaf spot. In: Hartman GL, Sinclair JB, Rupe JC (eds.) Compendium of Soybean Diseases. 4th Ed. St. Paul. MN, USA, APS Press, pp 20–22
Ploper LD, González V, Gálvez R, Devani M, Ledesma F (2000) La mancha ojo de rana otra enfermedad limitante del cultivo de la soja. EEAOC-Avance Agroindustrial:9–12
R Core Team (2017) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
Reddy MV, Singh KB (1984) Eradication of Ascochyta rabiei from chickpea seeds with thiabendazole. International Chickpea Newsletter 10:17–18
Roy KW, Baird RE, Abney TS (2001) A review of soybean (Glycine max) seed, pod, and flower mycofloras in North America, with methods and a key for identification of selected fungi. Mycopathologia 150:15–27
Scandiani MM, Carmona MA (2009) Boletín Técnico: Mancha de Ojo de Rana (Cercospora sojina) en semillas de soja. Asociación de Laboratorios Agropecuarios Privados http://www.laboratoriosalap.com.ar/notas/mor_soja.htm (verified March, 2018)
Scandiani MM, Ferri M, Carmona MA, Ruberti DS, Luque A, Tartabini M (2009a) Presencia de Cercospora sojina Hara en semillas de soja. Métodos para su detección. In: XIII Jornadas Fitosanitarias Argentinas, Libro de Resúmenes. Santiago del Estero, Argentina, p 93
Scandiani MM, Ferri M, Carmona MA, Ruberti DS, Tartabini M, Luque A (2009b) Evaluación de fungicidas para el control de Cercospora sojina Hara en semillas de soja. In: XIII Jornadas Fitosanitarias Argentinas, Libro de Resúmenes. Santiago del Estero, Argentina, p 80
Scandiani MM, Luque AG, Carmona MA, Formento AN, Souza J de, Asciutto K, Rosenzvaig ME, Celotto A, Cariacedo C, Souilla M, Tommasi M, Elizalde R, Grub A, Firpo E, Piccone R, Sola R, Sarmiento M, Petinari A, Pacios F, Martínez V, de Pablo, MC (2009c) Determinación de Cercospora sojina en semilla de soja. Evaluación de sanidad de semillas inter-laboratorios. Análisis de semillas 3:96–98
Scandiani MM, Luque AG (2009) Identificación de Patógenos en Semilla de Soja. In: Guillermo R (ed) Suplemento Análisis de Semillas. Rosario, Argentina. pp. 148. pp 1852–5024
Scandiani MM, Ferri M, Ferrari B, Formento N, Carmona M, Luque A, Balatti P (2012) First report of races 11 and 12 of Cercospora sojina, the causal agent of soybean frogeye leaf spot, in Argentina. Plant Disease 96:1067–1067
Singh T, Sinclair JB (1985) Histopathology of Cercospora sojina in soybean seeds. Phytopathology 75:185–189
Yorinori JT (1989) Frogeye leaf spot of soybean (Cercospora sojina Hara). In: IV Conferencia Mundial de Investigación en Soja, Libro de Resúmenes. Buenos Aires, Argentina, pp 1275–1283
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This work was financially supported by the University of Buenos Aires, Argentina through Project UBACyT 20020130100604BA.
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Section Editor: Adam H. Sparks
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Sautua, F.J., Scandiani, M.M., Gordo, M. et al. Detection and chemical control of Cercospora sojina infecting soybean seed in Argentina. Trop. plant pathol. 43, 552–558 (2018). https://doi.org/10.1007/s40858-018-0245-x
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DOI: https://doi.org/10.1007/s40858-018-0245-x