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Human Osteoblast Cell-Ti6Al4V Metal Alloy Interactions Under Varying Cathodic Potentials: A Pilot Study

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Abstract

Surface oxide plays an important role in the biocompatibility of metallic implants. The stability of this surface oxide varies depending on the surrounding environment of the implant. Numerous electrochemical processes may take place during formation and depletion of an oxide layer on the surface through corrosion. This shifts the open circuit potential (OCP) of titanium alloy (generally at ~150 mV vs. SCE) to be more cathodic. Therefore, the relation between cellular response and cathodic potential was investigated in this study first time for the MG63 cells type on the Ti-6Al-4V surface. Initially, the surface was polished and brought to a mirror finish. Then cells were cultured on top of such surfaces kept at two different static cathodic potentials (−300 and −600 mV vs. SCE) for 24 h. The experiments were facilitated using a custom glass corrosion chamber. With shifts toward more cathodic potentials than OCP, it was found that the well-spread viable cells rendered to small, rounded-up, unhealthy cells. This study suggested the apoptotic MG63 cell death due to electrochemical reactions and their products generated under cathodic potentials.

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References

  1. Pohler OE (2000) Unalloyed titanium for implants in bone surgery. Injury 31:D7–D13

    Article  Google Scholar 

  2. Wintermantel E, Ha S-W (2008) Medizintechnik: life science engineering. Springer, Berlin

    Book  Google Scholar 

  3. Navarro M, Michiardi A, Castano O, Planell J (2008) Biomaterials in orthopaedics. J R Soc Interface 5(27):1137–1158

    Article  Google Scholar 

  4. Sivan S, Kaul S, Gilbert JL (2013) The effect of cathodic electrochemical potential of Ti-6Al-4V on cell viability: voltage threshold and time dependence. J Biomed Mater Res B Appl Biomater 101(8):1489–1497

    Article  Google Scholar 

  5. Keegan GM, Learmonth ID, Case C (2008) A systematic comparison of the actual, potential, and theoretical health effects of cobalt and chromium exposures from industry and surgical implants. Crit Rev Toxicol 38(8):645–674

    Article  Google Scholar 

  6. Spangehl MJ, Younger A, Masri B, Duncan C (1998) Diagnosis of infection following total hip arthroplasty. Instr Course Lect 47:285–295

    Google Scholar 

  7. Gilbert J (2011) Electrochemical behavior of metals in the biological milieu. Compr Biomater 1(1):103

    Google Scholar 

  8. Jacobs JJ, Gilbert JL, Urban RM (1998) Current concepts review-corrosion of metal orthopaedic implants*. J Bone Joint Surg 80(2):268–282

    Article  Google Scholar 

  9. Gittens R, Olivares-Navarrete R, Tannenbaum R, Boyan B, Schwartz Z (2011) Electrical implications of corrosion for osseointegration of titanium implants. J Dent Res 90(12):1389–1397

    Article  Google Scholar 

  10. Gilbert JL, Mehta M, Pinder B (2009) Fretting crevice corrosion of stainless steel stem–CoCr femoral head connections: comparisons of materials, initial moisture, and offset length. J Biomed Mater Res B Appl Biomater 88(1):162–173

    Article  Google Scholar 

  11. Ehrensberger MT, Sivan S, Gilbert JL (2010) Titanium is not “the most biocompatible metal” under cathodic potential: the relationship between voltage and MC3T3 preosteoblast behavior on electrically polarized cpTi surfaces. J Biomed Mater Res A 93(4):1500–1509

    Google Scholar 

  12. Contu F, Elsener B, Böhni H (2004) A study of the potentials achieved during mechanical abrasion and the repassivation rate of titanium and Ti6Al4V in inorganic buffer solutions and bovine serum. Electrochim Acta 50(1):33–41

    Article  Google Scholar 

  13. Gilbert JL, Zarka L, Chang E, Thomas CH (1998) The reduction half cell in biomaterials corrosion: oxygen diffusion profiles near and cell response to polarized titanium surfaces. J Biomed Mater Res 42(2):321–330

    Article  Google Scholar 

  14. Venugopalan R, Weimer JJ, George MA, Lucas LC (2000) The effect of nitrogen diffusion hardening on the surface chemistry and scratch resistance of Ti-6Al-4V alloy. Biomaterials 21(16):1669–1677

    Article  Google Scholar 

  15. Haeri M, Wöllert T, Langford GM, Gilbert JL (2012) Electrochemical control of cell death by reduction-induced intrinsic apoptosis and oxidation-induced necrosis on CoCrMo alloy in vitro. Biomaterials 33(27):6295–6304

    Article  Google Scholar 

  16. Haeri M, Wöllert T, Langford GM, Gilbert JL (2013) Voltage-controlled cellular viability of preosteoblasts on polarized cpTi with varying surface oxide thickness. Bioelectrochemistry 94:53–60

    Article  Google Scholar 

  17. Haeri M, Gilbert JL (2013) Study of cellular dynamics on polarized CoCrMo alloy using time-lapse live-cell imaging. Acta Biomater 9(11):9220–9228

    Article  Google Scholar 

  18. Ciolko AA, Tobias M, Ehrensberger MT (2015) The effect of fretting associated periodic cathodic potential shifts on the electrochemistry and in vitro biocompatibility of commercially pure titanium. J Biomed Mater Res B Appl Biomater 104(8):1591–1601

    Article  Google Scholar 

  19. Kalbacova M, Roessler S, Hempel U, Tsaryk R, Peters K, Scharnweber D, Kirkpatrick JC, Dieter P (2007) The effect of electrochemically simulated titanium cathodic corrosion products on ROS production and metabolic activity of osteoblasts and monocytes/macrophages. Biomaterials 28(22):3263–3272

    Article  Google Scholar 

  20. Ehrensberger MT, Gilbert JL (2010) The effect of static applied potential on the 24-hour impedance behavior of commercially pure titanium in simulated biological conditions. J Biomed Mater Res B Appl Biomater 93(1):106–112

    Google Scholar 

  21. Clechet P, Martelet C, Martin J, Olier R (1979) Photoelectrochemical behaviour of TiO 2 and formation of hydrogen peroxide. Electrochim Acta 24(4):457–461

    Article  Google Scholar 

  22. Chiarugi P, Pani G, Giannoni E, Taddei L, Colavitti R, Raugei G, Symons M, Borrello S, Galeotti T, Ramponi G (2003) Reactive oxygen species as essential mediators of cell adhesion the oxidative inhibition of a FAK tyrosine phosphatase is required for cell adhesion. J cell biol 161(5):933–944

    Article  Google Scholar 

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Acknowledgements

We are very thankful to Richard Frueh, Physical Sciences Machine Shop, UIC Department of Physics, Brian D. Schwandt, Glass shop, UIC Department of Chemistry, for their aid and assistance in designing the corrosion chamber for this work, and the National Science Foundation (CBET #1067424 and DMR #1307052) for the financial support.

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Authors and Affiliations

  1. Department of Bioengineering, University of Illinois at Chicago, Chicago, IL, USA

    Arghya K. Bishal, John Grotberg & Christos G. Takoudis

  2. Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, Chicago, IL, USA

    Cortino Sukotjo

  3. Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL, USA

    Mathew T. Mathew

  4. Department of Chemical Engineering, University of Illinois at Chicago, Chicago, IL, USA

    Christos G. Takoudis

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  1. Arghya K. Bishal
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  2. John Grotberg
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  5. Christos G. Takoudis
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Correspondence to Christos G. Takoudis.

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Bishal, A.K., Grotberg, J., Sukotjo, C. et al. Human Osteoblast Cell-Ti6Al4V Metal Alloy Interactions Under Varying Cathodic Potentials: A Pilot Study. J Bio Tribo Corros 3, 40 (2017). https://doi.org/10.1007/s40735-017-0101-4

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  • DOI: https://doi.org/10.1007/s40735-017-0101-4

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