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GLP-1 receptor agonist increases the expression of CTRP3, a novel adipokine, in 3T3-L1 adipocytes through PKA signal pathway

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Abstract

Objective

To investigate the effects of Exendin-4 (Ex-4), a glucagon-like peptide-1 (GLP-1) receptor agonist, on the expression of C1q/TNF-related protein-3 (CTRP3), a novel adipokine, in 3T3-L1 adipocytes. The role of protein kinase A (PKA) signal pathway in the effects was also investigated.

Methods

The mRNA and protein expressions of CTRP3 in 3T3-L1 adiocytes were detected by real-time polymerase chain reaction and western blot, respectively. Exendin-fragment 9-39 (Ex-9), a specific GLP-1 receptor antagonist, and H89, a selective antagonist of PKA, were used to confirm the signal pathway of Ex-4 on CTRP3.

Results

2.5 or 5.0 nmol/l Ex-4 treatment for 8 h increased the expressions of CTRP3 mRNA and protein as well as PKA protein in 3T3-L1 adipocytes significantly, while Ex-9 or H89 blocked the up-regulation of CTRP3 expression induced by Ex-4 completely.

Conclusion

GLP-1 receptor agonist increases the expression of CTRP3 mRNA and protein in 3T3-L1 adipocytes via PKA signal pathway.

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Acknowledgments

This research was supported by the Fundamental Research Funds for the Central University (4101017) and Natural Science Foundation of Zhongnan Hospital Wuhan University (2013A05).

Conflict of interest

None of the authors have any potential conflicts of interest associated with this research.

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Authors and Affiliations

  1. Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    X. Li, M. Yang, Y. Wu, S. Sun & J. Sun

  2. Department of Internal Medicine, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    L. Jiang

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  1. X. Li
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  2. L. Jiang
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  3. M. Yang
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  4. Y. Wu
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Correspondence to X. Li.

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Li, X., Jiang, L., Yang, M. et al. GLP-1 receptor agonist increases the expression of CTRP3, a novel adipokine, in 3T3-L1 adipocytes through PKA signal pathway. J Endocrinol Invest 38, 73–79 (2015). https://doi.org/10.1007/s40618-014-0156-8

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  • DOI: https://doi.org/10.1007/s40618-014-0156-8

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