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Moisture stress induced anatomical, morpho-physiological and molecular changes in chickpea genotypes

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Abstract

Drought stress is usually preceded by moisture stress conditions that triggers the plant to acclimate and adapt for the upcoming stress conditions. In the present study, two contrasting genotypes, ICC 4958 (drought-resistant) and CSJ 513 (drought-sensitive), were lysimetrically screened under normal and moisture-stressed conditions at the flower initiation stage. The study recorded various phenological, morpho-physiological and anatomical attributes to analyse any damage under reduced moisture conditions. The genotype ICC 4958 exhibited early flowering to avoid stress and expanded its root surface area by 2.7 times during the flower initiation stage under moisture stress conditions. However, both genotypes recorded cellular damage i.e. membrane permeability index (51.07%) and decreased leaf viability (34.70%) under mositure stress, with CSJ 513 being more affected than ICC 4958. Root analysis via SEM evidenced thickening of parenchyma and increased metaxylem vessels in the root of ICC 4958. Semi-quantitative PCR amplification studies found an expression of a specific gene, LEA6, in ICC 4958. A phylogenetic study using the homologous sequences reveals the distinctness of the two genes from the other family members. We detected five and two highly conserved regions in LEA3 and LEA6 genes respectively, through multiple sequence alignment, which might play a role in biotic stress tolerance.

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Authors and Affiliations

  1. Department of Agronomy, Punjab Agricultural University, Ludhiana, India

    Norah Johal

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India

    Jagmeet Kaur, Sarvjeet Singh & Inderjit Singh

  3. Department of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India

    Yogesh Vikal & Inderjit Yadav

Authors
  1. Norah Johal
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  2. Jagmeet Kaur
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  3. Yogesh Vikal
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  4. Inderjit Yadav
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  5. Sarvjeet Singh
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  6. Inderjit Singh
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Contributions

NJ was involved in the field and lysimetric screening experiments. She collected and analyzed the whole data through statistical analysis and was involved in basic framing and writing of research paper. JK mentored the student and was involved in the designing the whole Ph.D. research. The biotechnology part was performed under the guidance and of YV in the laboratories of school of agricultural biotechnology. IY was basically involved in dealing the bioinformatics part to design the phylogenetic tree with other legumes. The basic plant genetic material was procured from SS and IS plant breeders. Field experiments were conducted under the guidance of above-mentioned plant breeders.

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Correspondence to Norah Johal.

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Johal, N., Kaur, J., Vikal, Y. et al. Moisture stress induced anatomical, morpho-physiological and molecular changes in chickpea genotypes. Plant Physiol. Rep. 28, 378–387 (2023). https://doi.org/10.1007/s40502-023-00740-w

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