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Salt and osmotic stress response of tobacco plants overexpressing Lepidium latifolium L. Ran GTPase gene

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Abstract

Our earlier study has identified Lepidium latifolium Ran (LlaRan) as a cold stress downregulated gene from L. latifolium, a cold desert plant. Further, independent transgenic tobacco (Nicotiana tabacum L. var Xanthi) lines overexpressing LlaRan were developed for functional characterization of the gene. We analysed the tolerance of tobacco plants over expressing LlaRan gene to salt and PEG stresses at germination which represents a critical stage in life cycle of the plants. Germination performance of the four independent transgenic lines (T1 generation) were assessed for germination under distilled water, NaCl (150 mM) and osmotic (15% PEG 4000) stresses. The emergence of radical, plumule and cotyledonary leaf were recorded for 10 days on daily basis and its rate assessed in terms of Timson’s index. It was observed that the germination of wild type tobacco seeds was better than the assessed transgenic lines. It may be inferred that the transgenic lines seeds under study were more sensitive to salt and drought stress elucidating their hypersensitivity at germination level.

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Acknowledgements

Financial support to VBS by Defence Research Development Organization, HQ, New Delhi is duly acknowledged.

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Author notes

  1. Vimlendu Bhushan Sinha

    Present address: Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida, Uttar Pradesh, 201306, India

Authors and Affiliations

  1. Defence Institute of Bio-Energy Research, Goraparao, P.O. Arjunpur, Haldwani, 263139, India

    Vimlendu Bhushan Sinha, Atul Grover & Patade Vikas Yadav

  2. Department of Biotechnology, Kumaun University, Bhimtal Campus, Bhimtal, Uttarakhand, 263136, India

    Veena Pande

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  1. Vimlendu Bhushan Sinha
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  2. Atul Grover
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  3. Patade Vikas Yadav
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  4. Veena Pande
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Correspondence to Vimlendu Bhushan Sinha.

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Sinha, V.B., Grover, A., Yadav, P.V. et al. Salt and osmotic stress response of tobacco plants overexpressing Lepidium latifolium L. Ran GTPase gene. Ind J Plant Physiol. 23, 494–498 (2018). https://doi.org/10.1007/s40502-018-0396-2

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  • DOI: https://doi.org/10.1007/s40502-018-0396-2

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