Abstract
The poor mineral bioavailability due to high phytic acid content in soybean (Glycine max) reduces its nutraceutical significance. Thus, in the present study effect of soaking and germination was investigated in two Indian soybean cultivars, viz., Pusa 9712 and Kalitur for their phytate and total and HCl-extractable iron, zinc and calcium content. Soaking for 12 h reduced the phytate content by 10–13 %, while soaking, followed by germination for 72 h reduced the phytate levels further by 46–65 % and increased the extractability of iron, zinc and calcium by 25–36 %. A negative correlation (P < 0.05) was thus observed with the antinutrient phytic acid and the HCl-extractability of iron, zinc and calcium in the sprouted soybean seeds.
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Badau, M. H., Nkama, I., & Jideani, I. A. (2005). Phytic acid content and hydrochloric acid extractability of minerals in pearl millet as affected by germination time and cultivar. Food Chemistry, 92, 425–435.
Bishnoi, S., & Khetarpaul, N. (1995). Effect of various domestic processing and cooking methods on the HCl-extractability of minerals from pea seeds. Die Nahrung, 39(5), 514–520.
Bishnoi, S., Khetarpaul, N., & Yadav, R. K. (1994). Effect of domestic processing and cooking methods on phytic acid and polyphenol contents of pea cultivars (pisumsativum). Plant Food for Human Nutrition, 45, 381–388.
Gibson, R. S., Hotz, C., Temple, C., Yeudall, F., Mtitimuni, B., & Ferguson, E. (2000). Dietary strategies to combat deficiencies of iron, zinc, and vitamin A in developing countries: Development, implementation, monitoring, and evaluation. Food and Nutrition Bulletin, 21, 219–231.
Grewal, A., & Jood, S. (2006). Effect of processing treatments on nutritional antinutritional contents of green gram. Journal of Food Biochemistry, 30, 535–546.
Hotz, C., Gibson, R. S., & Temple, L. (2001). A home-based method to reduce phytate content and increase bioavailability in maize-based complementary diets. International Journal of Food Science and Nutrition, 52, 133–142.
Kataria, A., Chauhan, B., & Punia, D. (1989). Antinutrients and protein digestibility (in vitro) of mungbean as affected by domestic processing and cooking. Food Chemistry, 32, 9–17.
Kumar, K. G., Venkataraman, L. V., Jaya, T. V., & Krishnamurthy, K. S. (1978). Cooking characteristics of some germinated legumes: changes in phytins, Ca++, Mg++ and pectins. Journal of Food Science, 43, 85–89.
Larsson, M., Minekus, M., & Havenaar, R. (1997). Estimation of the bioavailability of iron and phosphorus in cereals using a dynamic in vitro gastrointestinal model. Journal of Food Science and Agriculture, 74, 99–106.
Lestienne, I., Besancon, P., Caporiccio, B., Lullien-Pellerin, V., & Treche, S. (2005a). Iron and zinc in vitro availability in pearl millet flours (Pennisetumglaucum) with varying phytate, tannin, and fiber contents. Journal of Agriculture and Food Chemistry, 53(8), 3240–3247.
Lestienne, I., Icard-Verniere, C., Mouquet, C., Picq, C., & Treche, S. (2005b). Effects of soaking whole cereal and legume seeds on iron, zinc and phytate contents. Food Chemistry, 89(3), 421–425.
Lestienne, I., Mouquet-Rivier, C., Icard-Verniere, C., Rochette, I., & Treche, S. (2005c). The effects of soaking of whole, dehulled and ground millet and soybean seeds on phytate degradation and Phy/Fe and Phy/Zn molar ratios. Introductory Journal of Food Science and Technology, 40(4), 391–399.
Mahgoub, S. E. O., & Elhag, S. A. (1998). Effect of milling, soaking, malting, heat-treatment and fermentation on phytate level of four Sudanese sorghum cultivars. Food Chemistry, 61, 77–80.
Mandal, N. C., & Biswas, B. B. (1970). Metabolism of Inositol Phosphates. Plant Physiology, 45, 4–7.
Raboy, V., Dickinson, D. B., & Below, F. E. (1984). Variation in seed total phosphorus, phytic acid, zinc, calcium, magnesium, and protein among lines of Glycine max and G. soja. Crop Science, 24, 431–434.
Salunkhe, D. K., Chavan, D. K., & Kadam, S. S. (1986). Pigeon pea as an important food source. CRC Critical Review Food Science, 23, 103–145.
Shimelis, E. A., & Rakshit, S. K. (2007). Effect of processing on antinutrients and in vitro protein digestibility of kidney bean (Phaseolus vulgaris L.) varieties grown in East Africa. Food Chemistry, 103, 161–172.
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Kumari, S., Krishnan, V., Jolly, M. et al. Reduction in phytate levels and HCl-extractability of divalent cations in soybean (Glycine max L.) during soaking and germination. Ind J Plant Physiol. 20, 44–49 (2015). https://doi.org/10.1007/s40502-014-0132-5
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DOI: https://doi.org/10.1007/s40502-014-0132-5