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Phenylethanoid glycoside from Forsythia koreana (Oleaceae) flowers shows a neuroprotective effect

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Abstract

A repeated column chromatography for Forsythia koreana Nakai. flowers afforded a phenylethane glycoside, which was identified as 8′-(3,4-dihydroxyphenyl)ethyl-O-α-l-rhamnopyranosyl-(1 → 4)-2-O-(E)-caffeoyl-α-l-arabinopyranoside based on spectroscopic analyses of 1D NMR (1H-NMR, 13C-NMR, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC), FAB/MS, specific rotation, and IR spectrometry. This compound was isolated from F. koreana flower for the first time in this study. The compound showed scavenge activities in ORAC and DPPH radicals with the values of 0.83 ± 0.01 and 84.66 ± 0.75% (at the concentration of 500 ppm), respectively. It also inhibited production of NO for RAW 264.7 stimulated by LPS and protected oxidative stress induced by glutamate in HT22-immortalized hippocampal. The IC50 on inhibition of NO production and EC50 value on protective efficacy of oxidative stress were 78.72 ± 1.43 μM and 176.25 ± 7.23 μM, respectively. These results demonstrate that the phenylethanoid glycoside might be useful as a neuroprotective agent because of their antioxidant, anti-inflammatory, and neuroprotective effects.

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Acknowledgements

RDA supported this study through “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ011333022017).”

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Authors and Affiliations

  1. Graduate School of Biotechnology and Department of Oriental Medicine Biotechnology, Kyung Hee University, Yongin, 17104, Republic of Korea

    Yeong-Geun Lee, Jung Eun Gwag, Hyoung-Geun Kim, Jung-Hwan Ko, So-Hyun Park & Nam-In Baek

  2. Biological and Genetic Resources Utilization Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

    Kyeong-Hwa Seo

  3. College of Pharmacy, Chosun University, Gwnagju, 61452, Republic of Korea

    Dong-Sung Lee

  4. Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Republic of Korea

    Dae Young Lee

  5. Department of Life and Nanopharmaceutical Science, Kyung Hee University, Seoul, 02447, Republic of Korea

    In Ho Jung

Authors
  1. Yeong-Geun Lee
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  2. Kyeong-Hwa Seo
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  3. Dong-Sung Lee
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  8. Dae Young Lee
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  9. In Ho Jung
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  10. Nam-In Baek
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Contributions

Y-G L, K-H S, H-G K, and N-I B prepared the material. Y-G L and N-I B designed the experiment and wrote the paper. Y-G L, K-H S, J-E G, H-G K, J-H K, S-A J, and D-Y L carried out isolated experiment. Y-G L and N-I B Identified all compounds. D-S L and I-H J carried out antioxidant and anti-inflammatory experiments.

Corresponding author

Correspondence to Nam-In Baek.

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Lee, YG., Seo, KH., Lee, DS. et al. Phenylethanoid glycoside from Forsythia koreana (Oleaceae) flowers shows a neuroprotective effect. Braz. J. Bot 41, 523–528 (2018). https://doi.org/10.1007/s40415-018-0468-6

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  • DOI: https://doi.org/10.1007/s40415-018-0468-6

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