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PM2.5-associated bacteria in ambient air: Is PM2.5 exposure associated with the acquisition of community-acquired staphylococcal infections?

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Abstract

Particulate matter (PM), a major component of air pollution, is an important carrier medium of various chemical and microbial compounds. Air pollution due to PM could increase the level of bacteria and associated adverse health effects. Staphylococci as important opportunistic pathogens that cause hospital- and community-acquired infections may transmit through air. This study aimed to obtain knowledge about the concentration of airborne bacteria as well as staphylococci associated with particulate matter with a diameter of less than 2.5 micrometers (PM2.5) in ambient air. The impact of meteorological factors including ultraviolet (UV) index, wind speed, temperature, and moisture on microbial concentrations was also investigated. Quartz filters were used to collect PM2.5 and associated bacteria in ambient air of a semiarid area. Airborne bacteria were quantified by culture method and Staphylococcus species identified by molecular methods. The mean (SD) concentration of PM2.5 and airborne bacteria was 64.83 (24.87) µg/m3 and 38 (36) colony forming unit (CFU)/m3, respectively. The results showed no significant correlation between the levels of PM2.5 and concentrations of bacteria (p < 0.05). Staphylococcus species were detected in 8 of 37 (22%) samples in a concentration from 3 to 213 CFU/m3. S. epidermidis was detected with the highest frequency followed by S. gallinarum and S. hominis, but S. aureus and methicillin-resistant Staphylococcus aureus (MRSA) were not detected. No significant correlation between the concentrations of bacteria with meteorological parameters was observed (p < 0.05). Our finding showed that, although the study area is sometimes subject to air pollution from PM2.5, the concentration of PM2.5- associated bacteria is relatively low. According to the results, PM2.5 may not be a source of community-associated staphylococcal infections.

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References

  1. Abulreesh H, Organji S. The prevalence of multidrug-resistant staphylococci in food and the environment of Makkah, Saudi Arabia. Res J Microbiol. 2011;6(6):510–23.

    Article  Google Scholar 

  2. Beggs C. The airborne transmission of infection in hospital buildings: fact or fiction? Indoor Built Environ. 2003;12(1–2):9–18.

    Article  Google Scholar 

  3. Madsen AM, Moslehi-Jenabian S, Islam MZ, Frankel M, Spilak M, Frederiksen MW. Concentrations of Staphylococcus species in indoor air as associated with other bacteria, season, relative humidity, air change rate, and S. aureus-positive occupants. Environ Res. 2018;160:282–91.

    Article  CAS  Google Scholar 

  4. Calfee DP, Salgado CD, Milstone AM, Harris AD, Kuhar DT, Moody J, et al. Strategies to prevent methicillin-resistant staphylococcus aureus transmission and infection in acute care hospitals: 2014 update. Infect Control Hosp Epidemiol. 2016;35(S2):S108–S132.

    Article  Google Scholar 

  5. Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clin Microbiol Rev. 2014;27(4):870–926.

    Article  Google Scholar 

  6. Madsen AM, Kurdi I, Feld L, Tendal K. Airborne MRSA and total staphylococcus aureus as associated with particles of different sizes on pig farms. Ann Work Expo Health. 2018;62(8):966–77.

    Article  CAS  Google Scholar 

  7. Faridi S, Naddafi K, Kashani H, Nabizadeh R, Alimohammadi M, Momeniha F, et al. Bioaerosol exposure and circulating biomarkers in a panel of elderly subjects and healthy young adults. Sci Total Environ. 2017;593–594:380–9.

    Article  Google Scholar 

  8. Aziz AA, Lee K, Park B, Park H, Park K, Choi I-G, et al. Comparative study of the airborne microbial communities and their functional composition in fine particulate matter (PM2. 5) under non-extreme and extreme PM2. 5 conditions. Atmos Environ. 2018;194:82–92.

    Article  Google Scholar 

  9. Shamsipour M, Hassanvand MS, Gohari K, Yunesian M, Fotouhi A, Naddafi K, et al. National and sub-national exposure to ambient fine particulate matter (PM2. 5) and its attributable burden of disease in Iran from 1990 to 2016. Environ Pollut. 2019;255:113173.

    Article  CAS  Google Scholar 

  10. Xu C, Wei M, Chen J, Zhu C, Li J, Xu X, et al. Profile of inhalable bacteria in PM2. 5 at Mt. Tai, China: Abundance, community, and influence of air mass trajectories. Ecotoxicol Environ Saf. 2019;168:110–9.

    Article  CAS  Google Scholar 

  11. Hadei M, Nazari SSH, Yarahmadi M, Kermani M, Farhadi M, Shahsavani A. Estimation of gender-specific lung cancer deaths due to exposure to PM2. 5 in 10 cities of Iran during 2013–2016: A modeling approach. Int J Cancer Manag. 2013;10(8):1–8.

    Google Scholar 

  12. Goudarzi G, Shirmardi M, Naimabadi A, Ghadiri A, Sajedifar J. Chemical and organic characteristics of PM2. 5 particles and their in-vitro cytotoxic effects on lung cells: The Middle East dust storms in Ahvaz, Iran. Sci Total Environ. 2019;655:434–45.

    Article  CAS  Google Scholar 

  13. Polymenakou PN, Mandalakis M, Stephanou EG, Tselepides A. Particle size distribution of airborne microorganisms and pathogens during an intense African dust event in the eastern Mediterranean. Environ Health Perspect. 2007;116(3):292–6.

    Article  Google Scholar 

  14. Hussey SJ, Purves J, Allcock N, Fernandes VE, Monks PS, Ketley JM, et al. Air pollution alters Staphylococcus aureus and Streptococcus pneumoniae biofilms, antibiotic tolerance and colonisation. Environ Microbiol. 2017;19(5):1868–80.

    Article  CAS  Google Scholar 

  15. Karanasiou A, Moreno N, Moreno T, Viana M, De Leeuw F, Querol X. Health effects from Sahara dust episodes in Europe: literature review and research gaps. Environ Int. 2012;47:107–14.

    Article  CAS  Google Scholar 

  16. Shamsizadeh Z, Nikaeen M, Esfahani BN, Mirhoseini SH, Hatamzadeh M, Hassanzadeh A. Detection of antibiotic resistant Acinetobacter baumannii in various hospital environments: potential sources for transmission of Acinetobacter infections. Environ Health Prev Med. 2017;22(1):44.

    Article  Google Scholar 

  17. Noguchi N, Suwa J, Narui K, Sasatsu M, Ito T, Hiramatsu K, et al. Susceptibilities to antiseptic agents and distribution of antiseptic-resistance genes qacA/B and smr of methicillin-resistant Staphylococcus aureus isolated in Asia during 1998 and 1999. J Med Microbiol. 2005;54(6):557–65.

    Article  CAS  Google Scholar 

  18. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol. 1992;30(7):1654–60.

    Article  CAS  Google Scholar 

  19. Zhang K, McClure J-A, Elsayed S, Louie T, Conly JM. Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2005;43(10):5026–33.

    Article  CAS  Google Scholar 

  20. Baghal Asghari F, Nikaeen M, Mirhendi H. Rapid monitoring of Pseudomonas aeruginosa in hospital water systems: a key priority in prevention of nosocomial infection. FEMS Microbiol Lett. 2013;343(1):77–81.

    Article  Google Scholar 

  21. World Health Organization. Air quality guidelines: global update 2005: particulate matter, ozone, nitrogen dioxide, and sulfur dioxide. World Health Organization; 2006.

  22. Alghamdi MA, Shamy M, Redal MA, Khoder M, Awad AH, Elserougy S. Microorganisms associated particulate matter: a preliminary study. Sci Total Environ. 2014;479:109–16.

    Article  Google Scholar 

  23. Psoter KJ, De Roos AJ, Wakefield J, Mayer JD, Rosenfeld M. Air pollution exposure is associated with MRSA acquisition in young US children with cystic fibrosis. BMC Pulm Med. 2017;17(1):106.

    Article  Google Scholar 

  24. Qin T, Zhang F, Zhou H, Ren H, Du Y, Liang S, et al. High-Level PM2.5/PM10 Exposure is associated with alterations in the human pharyngeal microbiota composition. Front Microbiol. 2019;10(54).

  25. Fang Z, Ouyang Z, Zheng H, Wang X, Hu L. Culturable airborne bacteria in outdoor environments in Beijing,China. Microb Ecol. 2007;54(3):487–96.

    Article  Google Scholar 

  26. Fang Z, Yao W, Lou X, Hao C, Gong C, Ouyang Z. Profile and characteristics of culturable airborne bacteria in hangzhou, Southeast of China. Aerosol Air Quality Res. 2016;16(7):1690–700.

    Article  CAS  Google Scholar 

  27. Jeon EM, Kim HJ, Jung K, Kim JH, Kim MY, Kim YP, et al. Impact of Asian dust events on airborne bacterial community assessed by molecular analyses. Atmos Environ. 2011;45(25):4313–21.

    Article  CAS  Google Scholar 

  28. Ghosh B, Lal H, Srivastava A. Review of bioaerosols in indoor environment with special reference to sampling, analysis and control mechanisms. Environ Int. 2015;85:254–72.

    Article  Google Scholar 

  29. Du P, Du R, Ren W, Lu Z, Fu P. Seasonal variation characteristic of inhalable microbial communities in PM2. 5 in Beijing city, China. Sci Total Environ. 2018;610:308–15.

    Article  Google Scholar 

  30. Lu R, Li Y, Li W, Xie Z, Fan C, Liu P, et al. Bacterial community structure in atmospheric particulate matters of different sizes during the haze days in Xi’an, China. Sci Total Environ. 2018;637:244–52.

    Article  Google Scholar 

  31. Mirhoseini SH, Nikaeen M, Satoh K, Makimura K. Assessment of airborne particles in indoor environments: Applicability of particle counting for prediction of bioaerosol concentrations. Aerosol Air Qual Res. 2016;16(8):1903–10.

    Article  CAS  Google Scholar 

  32. Mirhoseini SH, Nikaeen M, Shamsizadeh Z, Khanahmad H. Hospital air: A potential route for transmission of infections caused by β-lactam–resistant bacteria. Am J Infect Control. 2016;44(8):898–904.

    Article  Google Scholar 

  33. Sivri N, Bağcıgil AF, Metiner K, Şeker DZ, Orak S, Durak SG, et al. Culturable airborne bacteria and isolation of methicillin-resistant coagulase-negative staphylococci from outdoor environments on European side of Istanbul, Turkey. Arch Environ Prot. 2016;42(3):77–86.

    Article  Google Scholar 

  34. Perez H, Michael K, Burstyn I. Airborne Methicillin-Resistant Staphylococcus aureus in Homes. Trends Microbiol. 2012;9:486–93.

    Google Scholar 

  35. Moon KW, Huh EH, Jeong HC. Seasonal evaluation of bioaerosols from indoor air of residential apartments within the metropolitan area in South Korea. Environ Monit Assess. 2014;186(4):2111–20.

    Article  CAS  Google Scholar 

  36. Gao M, Yan X, Qiu T, Han M, Wang X. Variation of correlations between factors and culturable airborne bacteria and fungi. Atmos Environ. 2016;128:10–9.

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by the Vice Chancellery for Research at the Isfahan University of Medical Sciences (Grant No 197050).

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Authors and Affiliations

  1. Student Research Committee and Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

    Hossein Karimi

  2. Department of Environmental Health Engineering, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

    Mahnaz Nikaeen, Sahar Gholipour & Maryam Hatamzadeh

  3. Environment Research Center, Research Institute for Primordial Prevention of Non-Communicable Diseases, Isfahan University of Medical Sciences, Isfahan, Iran

    Mahnaz Nikaeen & Yaghoub Hajizadeh

  4. Department of Statistics and Epidemiology, School of Health, Isfahan University of Medical Sciences, Isfahan, Iran

    Akbar Hassanzadeh

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  1. Hossein Karimi
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  2. Mahnaz Nikaeen
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  4. Maryam Hatamzadeh
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Correspondence to Mahnaz Nikaeen.

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Karimi, H., Nikaeen, M., Gholipour, S. et al. PM2.5-associated bacteria in ambient air: Is PM2.5 exposure associated with the acquisition of community-acquired staphylococcal infections?. J Environ Health Sci Engineer 18, 1007–1013 (2020). https://doi.org/10.1007/s40201-020-00522-8

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  • DOI: https://doi.org/10.1007/s40201-020-00522-8

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