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Xylanase as a Promising Biocatalyst: A Review on Its Production, Purification and Biotechnological Applications

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Xylanases are hydrolases that depolymerize the second most abundant polysaccharide in plant cells xylan. The heterogeneous nature of xylan restricts its ability to be hydrolyzed, however xylanases, which have the ability to hydrolyze the heterogeneous β-1,4-glycoside bond and can overcome this. Xylanases are abundant in nature and are primarily produced by a variety of microorganisms, including fungi, algae, yeast, and bacteria. Microbial xylanases are ideal for a wide range of biotechnological and industrial applications due to their diverse substrate specificities and biochemical characteristics. The need for xylanases has increased globally as a result of their appropriateness for use in food and feed, paper and pulp, textiles, medicines, and lignocellulosic biorefinery. The present review gives an insight of into microbial xylanases, its source, production, purification and biotechnological applications. This review also provides some important aspects on advancement of xylanase production through recombinant DNA technology, mutation and cloning.

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Acknowledgements

Author is grateful to the Head, Department of Biotechnology, Maharaja Sriram Chandra Bhanjadeo University, Baripada, Odisha, India for providing his precious knowledge and helping the author writing the review properly.

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Author notes

  1. Hrudayanath Thatoi

    Present address: Centre for Industrial Biotechnology Research, Siksha ‘O’ Anusandhan, Deemed to be University, Bhubaneswar, Odisha, 751003, India

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  1. Department of Biotechnology, Maharaja Sriram Chandra Bahnjadeo University, Takatpur, Baripada, Odisha, 757003, India

    Arpita Sarangi & Hrudayanath Thatoi

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  1. Arpita Sarangi
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AS conducted major part of the review and contributed writing the original manuscript and formatting. HT supervised the whole work, conceptualized the review, guided the writing & editing of the manuscript.

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Correspondence to Hrudayanath Thatoi.

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Significance statement: The purpose of this study entitled “Xylanase a promising biocatalyst: a review on its production, purification and biotechnological applications” was to do a detailed review on classification, source, production, purification, genetic engineering insilico genomics and proteomics and studies on xylanase.

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Sarangi, A., Thatoi, H. Xylanase as a Promising Biocatalyst: A Review on Its Production, Purification and Biotechnological Applications. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. (2024). https://doi.org/10.1007/s40011-024-01567-7

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