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Effect of Olive Pomace Extract on the Gut Health in Weaned Piglets

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The post-weaning period is one of the most stressful phases in intensive pig farming, and it is often associated with inflammation and intestinal dysfunctions. Farmers as well as veterinarians of the pig production sector have used prophylactic antibiotics for decades in order to mitigate diseases that negatively affect intestinal health. The growing global concern about antimicrobial resistance has led to an increased interest in the use of natural products such as olive pomace (OP) extract. The aim of this study was to analyze the effects of dietary OP extract on the intestinal health of weanling piglets. Twelve weaned piglets were divided in control group, which received basal diet, and treated group, which received basal diet supplemented with 600 g OP/Ton. To study intestinal permeability, urine samples were collected to determine lactulose/mannitol ratio after receiving an oral dose of these sugars and plasma samples were collected to determine D-lactic acid concentrations. Also, plasma samples were used to evaluate the intestinal metabolism using citrulline as biomarker. After 15 d of treatment, 4 animals of each group were euthanized and ileal mucus and intestinal mucosa were collected to study adherence of bacteria to mucus and histomorphology, respectively. d-lactate and citrulline concentrations were significantly lower and higher, respectively in the treated group compared with the control group. Intestinal absorptive area, goblet cells counts and adherence of E. coli to intestinal mucus were significantly higher in the treated group compared to the control group. Our results showed that OP induces positive changes in the intestinal health parameters and should be considered an alternative to prophylactic use of antibiotics during the post-weaning period.

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References

  1. Bischoff SC, Giovanni B, Buurman W, Ockhuizen T, Schulzke JD, Serino M, Tilg H, Watson A, Wells JM (2014) Intestinal permeability—a new target for disease prevention and therapy. BMC Gastroenterol 14(1):1–25

    Article  Google Scholar 

  2. Campbell JM, Crenshaw JD, Polo J (2013) The biological stress of early weaned piglets. J Anim Sci Biotechnol 4(1):19. https://doi.org/10.1186/2049-1891-4-19

    Article  PubMed  PubMed Central  Google Scholar 

  3. Moeser AJ, Calvin SP, Mrigendra R (2017) Weaning stress and gastrointestinal barrier development: implications for lifelong gut health in pigs. Anim Nutr 3(4):313–321. https://doi.org/10.1016/j.aninu.2017.06.003

    Article  PubMed  PubMed Central  Google Scholar 

  4. Valenzuela-Grijalva NV, Pinelli-Saavedra A, Muhlia-Almazan A, Domínguez-Díaz D, González-Ríos H (2017) Dietary inclusion effects of phytochemicals as growth promoters in animal production. J Anim Sci Technol 59:8

    Article  PubMed  PubMed Central  Google Scholar 

  5. Martínez G, Diéguez SN, Fernández Paggi MB, Riccio MB, Perez Gaudio DS, Rodríguez E, Amanto FA, Tapia MO, Soraci AL (2019) Effect of fosfomycin, Cynara scolymus extract, deoxynivalenol and their combinations on intestinal health of weaned piglets. Anim Nutr 5:386–395

    Article  PubMed  PubMed Central  Google Scholar 

  6. Dieguez SN, Decundo JM, Martínez G, Amanto FA, Bianchi CP, Pérez-Gaudio DS, Soraci AL (2021) Effect of Dietary Oregano Lippia origanoides and Clover Eugenia caryophillata essential oils’ formulations on intestinal health and performance of pigs. Planta Med Dec 10:101–123

    Google Scholar 

  7. Omonijo FA, Ni L, Gong J, Wang Q, Lahaye L, Yang C (2018) Essential oils as alternatives to antibiotics in swine production. Anim Nutr 4(2):126–136. https://doi.org/10.1016/j.aninu.2017.09.001

    Article  PubMed  Google Scholar 

  8. Gessner DK, Ringseis R, Eder K (2017) Potential of plant polyphenols to combat oxidative stress and inflammatory processes in farm animals. J Anim Physiol Anim Nutr (Berl) 101(4):605–628. https://doi.org/10.1111/jpn.12579

    Article  CAS  PubMed  Google Scholar 

  9. Márquez Martín A, de la Puerta VR, Fernández Arche A, Ruiz-Gutiérrez V (2006) Supressive effect of maslinic acid from pomace olive oil on oxidative stress and cytokine production in stimulated murine macrophages. Free Radic Res 40(3):295–302

    Article  PubMed  Google Scholar 

  10. Yap WH, Lim YM (2015) Mechanistic perspectives of Maslinic acid in targeting inflammation. Biochem Res Int. https://doi.org/10.1155/2015/279356

    Article  PubMed  PubMed Central  Google Scholar 

  11. Xun W, Shi L, Zhou H, Hou G, Cao T, Zhao C (2015) Effects of curcumin on growth performance, Jejunal mucosal membrane integrity, morphology and immune status in weaned piglets challenged with enterotoxigenic Escherichia coli. Int Immunopharmacol 27(1):46–52. https://doi.org/10.1016/j.intimp.2015.04.038

    Article  CAS  PubMed  Google Scholar 

  12. Gervasoni J, Schiattarella A, Giorgio V, Primiano A, Russo C, Tesori V, Scaldaferri F, Urbani A, Zuppi C, Persichilli S (2016) Validation of an LC–MS/MS method for urinary lactulose and mannitol quantification: results in patients with irritable bowel syndrome. Dis Mark 23:2705

    Google Scholar 

  13. Lansing M, Turner JM, Wizzard P, Lavallee CM, Lim DW, Muto M, Nation PN, Pencharz PB, Ball RO, Wales PW (2019) Plasma citrulline is not a biomarker for intestinal adaptation in short bowel syndrome, studied in piglets: a model for human neonates. Pediatr Surg 35(6):657–663. https://doi.org/10.1007/s00383-019-04475-4

    Article  Google Scholar 

  14. Jianfeng G, Weiming Z, Ning L, Fangnan L, Li T, Nan L, Jieshou L (2005) Serum citrulline is a simple quantitative marker for small intestinal enterocytes mass and absorption function in short bowel patients. J Surg Res 127(2):177–182. https://doi.org/10.1016/j.jss.2005.04.004

    Article  CAS  PubMed  Google Scholar 

  15. Berkeveld M, Langendijk P, Verheijden JH, Taverne MA, van Nes A, van Haard P, Koets AP (2008) Citrulline and intestinal fatty acid-binding protein: longitudinal markers of postweaning small intestinal function in pigs? J Anim Sci 86(12):3440–3449. https://doi.org/10.2527/jas.2008-1167

    Article  CAS  PubMed  Google Scholar 

  16. Wu G, Meininger CJ (2008) Analysis of citrulline, arginine, and methylarginines using high-performance liquid chromatography. Methods Enzymol 440(7):177–189. https://doi.org/10.1016/S0076-6879(07)00810-5

    Article  CAS  PubMed  Google Scholar 

  17. Kisielinski K, Willis S, Prescher A, Klosterhalfen B, Schumpelick V (2002) A simple new method to calculate small intestine absorptive surface in the rat. Clin Exp Med 2(3):131–135. https://doi.org/10.1007/s102380200018

    Article  CAS  PubMed  Google Scholar 

  18. Bai X, Liu X, Su Y (2000) Inhibitory effects of intestinal mucus on bacterial adherence to cultured intestinal epithelial cells after surface burns. Chin Med J 113(5):449–450

    CAS  PubMed  Google Scholar 

  19. Pohl CS, Medland JE, Mackey E, Edwards LL, Bagley KD, DeWilde MP, Williams KJ, Moeser AJ (2017) Early weaning stress induces chronic functional diarrhea, intestinal barrier defects, and increased mast cell activity in a porcine model of early life adversity. Neurogastroenterol Motil. https://doi.org/10.1111/nmo.13118

    Article  PubMed  PubMed Central  Google Scholar 

  20. Traina G (2019) Mast cells in gut and brain and their potential role as an emerging therapeutic target for neural diseases. Front Cell Neurosci 13:345. https://doi.org/10.3389/fncel.2019.00345

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Edelblum KL, Turner JR (2009) The tight junction in inflammatory disease: communication breakdown. Curr Opin Pharmacol 9(6):715–720. https://doi.org/10.1016/j.coph.2009.06.022

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Wang M, Zhang S, Zhong R, Wan F, Chen L, Liu L, Yi B, Zhang H (2021) Olive fruit extracts supplement improve antioxidant capacity via altering colonic microbiota composition in mice. Front Nutr 8:645099. https://doi.org/10.3389/fnut.2021.645099

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Liehr M, Mereu A, Pastor JJ, Quintela JC, Staats S, Rimbach G, Ipharraguerre IR (2017) Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota. PLoS ONE 12(3):e0174239. https://doi.org/10.1371/journal.pone

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Zheng P, Yu B, He J, Yu J, Mao X, Luo Y, Luo J, Huang Z, Tian G, Zeng Q, Che L, Chen D (2017) Arginine metabolism and its protective effects on intestinal health and functions in weaned piglets under oxidative stress induced by diquat. Br J Nutr 117(11):1495–1502. https://doi.org/10.1017/S0007114517001519

    Article  CAS  PubMed  Google Scholar 

  25. Hao Y, Xing M, Gu X (2021) Research progress on oxidative stress and its nutritional regulation strategies in pigs. Animals (Basel) 11(5):1384. https://doi.org/10.3390/ani11051384

    Article  PubMed  PubMed Central  Google Scholar 

  26. Crenn P, Messing B, Cynober L (2008) Citrulline as a biomarker of intestinal failure due to enterocyte mass reduction. Clin Nutr 27(3):328–339. https://doi.org/10.1016/j.clnu.2008.02.005

    Article  CAS  PubMed  Google Scholar 

  27. Marcelino G, Hiane PA, Freitas KC, Santana LF, Pott A, Donadon JR, Guimarães R (2019) Effects of Olive oil and its minor components on cardiovascular diseases, inflammation, and gut microbiota. Nutrients 11(8):1826. https://doi.org/10.3390/nu11081826

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Choct M (2009) Managing gut health through nutrition. Br Poult Sci 50(1):9–15

    Article  CAS  PubMed  Google Scholar 

  29. Awad WA, Ghareeb K, Paßlack N, Zentek J (2013) Dietary inulin alters the intestinal absorptive and barrier function of piglet intestine after weaning. Res Vet Sci 95(1):249–254. https://doi.org/10.1016/j.rvsc.2013.02.009

    Article  CAS  PubMed  Google Scholar 

  30. Zhang Y, Deng ZX, He ML, Luo XM, Liu JX, Wang H (2020) Olive extract ameliorates oxidative stress and in ammation, and protects intestinal villus and microbiota in piglets induced by lipopolysaccharides. Res Square. https://doi.org/10.21203/rs.3.rs-101171/v1

    Article  Google Scholar 

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Acknowledgements

This study was supported by CIVETAN-CONICET and 03-JOVIN-19H-SECAT-UNCPBA project.

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Authors and Affiliations

  1. Área Toxicología, Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de La Provincia de Buenos Aires, Tandil, Argentina

    Guadalupe Martínez, Susana Nelly Diéguez, Julieta María Decundo, Denisa Soledad Pérez Gaudio, Agustina Romanelli & Alejandro Luis Soraci

  2. Centro de Investigación Veterinaria de Tandil (CIVETAN, UNCPBA-CICPBA-CONICET), Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de La Provincia de Buenos Aires, 7000, Tandil, Buenos Aires, Argentina

    Guadalupe Martínez, Susana Nelly Diéguez, Julieta María Decundo, Denisa Soledad Pérez Gaudio, Agustina Romanelli & Alejandro Luis Soraci

  3. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Tandil, Buenos Aires, Argentina

    Guadalupe Martínez, Julieta María Decundo, Denisa Soledad Pérez Gaudio & Alejandro Luis Soraci

  4. Comisión de Investigaciones Científicas de La Provincia de Buenos Aires (CIC-PBA), Tandil, Argentina

    Susana Nelly Diéguez

  5. Área Producción Porcina, Departamento de Producción Animal, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de La Provincia de Buenos Aires, Tandil, Argentina

    Fabián Andrés Amanto

Authors
  1. Guadalupe Martínez
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  2. Susana Nelly Diéguez
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  3. Julieta María Decundo
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  4. Denisa Soledad Pérez Gaudio
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  6. Fabián Andrés Amanto
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  7. Alejandro Luis Soraci
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Correspondence to Guadalupe Martínez.

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Significance Statement

Early weaning increased intestinal permeability and decreased intestinal metabolism. Olive pomace extract was successfully incorporated into diet from weaned piglets and reduced the impact of post weaning stress on gut health.

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Martínez, G., Diéguez, S.N., Decundo, J.M. et al. Effect of Olive Pomace Extract on the Gut Health in Weaned Piglets. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 94, 359–367 (2024). https://doi.org/10.1007/s40011-023-01534-8

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