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Production of Agar-Derived Antioxidants and Single Cell Detritus from Gracilaria corticata Using Agarase from Microbulbifer sp. CMC-5

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Microbulbifer strain CMC-5 reportedly degrades multiple polysaccharides including agar. In present study, the agarase from the culture supernatant of Microbulbifer strain CMC-5 pregrown in artificial sea water-based medium containing agar was tenfold concentrated by ultrafiltration. The bacteriological agar-derived oligosaccharides obtained by agarase treatment were observed to depict reducing power, lipid peroxidation inhibition and hydroxyl radical scavenging activities. The oligosaccharides produced from agar by agarase treatment for 6 h demonstrated higher antioxidative activities as compared to agar oligosaccharides produced by 12-h agarase treatment. Further, the agar oligosaccharides demonstrated concentration-dependent augmentation of antioxidative activities. Since agar is a recognized safe food additive, the agar-derived oligosaccharides can be blended with food to augment its antioxidative property. Additionally, the agarase from strain CMC-5 in conjunction with Onozuka R-10 degraded Gracilaria corticata thalli to produce single cell detritus that can be used as aquaculture feed material reducing the dependency on artificial feeds.

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Acknowledgements

The authors are grateful to Goa University for providing the research fellowship during the tenure of this work. One of the authors acknowledges the Department of Biotechnology, New Delhi, India, for the DBT-JRF and DBT-SRF awards.

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Authors and Affiliations

  1. Department of Biotechnology, Goa University, Taleigao Plateau, Goa, 403206, India

    RaviChand Jonnadula, Md. Imran, Poonam Vashist & Sanjeev C. Ghadi

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  1. RaviChand Jonnadula
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  2. Md. Imran
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  3. Poonam Vashist
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  4. Sanjeev C. Ghadi
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Correspondence to Sanjeev C. Ghadi.

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Significance Statement

In the present study, enzymatically produced agar oligosaccharides depict broad range antioxidative properties that could be used as safe food additives. Additionally, agarase produces SCD from Gracilaria thalli that may be used as feed in aquaculture.

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Jonnadula, R., Imran, M., Vashist, P. et al. Production of Agar-Derived Antioxidants and Single Cell Detritus from Gracilaria corticata Using Agarase from Microbulbifer sp. CMC-5. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 73–78 (2020). https://doi.org/10.1007/s40011-019-01082-0

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  • DOI: https://doi.org/10.1007/s40011-019-01082-0

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