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Investigating Disease Controlling Ability of Brassica Volatiles and Their Compatibility with Trichoderma harzianum

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The present investigation aimed to test the in vitro toxicity of Brassica volatiles against soil-borne plant pathogens (Rhizoctonia solani Kuhn, Sclerotium rolfsii Sacc., Fusarium oxysporum f.sp. ciceris (Padwick) Matuo & K. Sato and Sclerotinia sclerotiorum (Lib.) de Bary) and biocontrol fungus Trichoderma harzianum Rifai. Moreover, the response of T. harzianum to toxic volatiles was also studied in terms of glucanase and chitinase gene expression. A strong fungistatic effect of B. alba treatment was recorded against all the test pathogens. Noticeably, S. sclerotiorum manifested least sensitivity among all the tested pathogens. All T. harzianum isolates were less sensitive as compared to the assayed pathogens. Based on the in vitro study involving 6 T. harzianum isolates, T55 and Th-R showed least, while T39 showed highest sensitivity to the volatiles. Interestingly, the patterns relating to the effect of volatiles on inoculum density of T. harzianum isolates were similar to their effect on fungus growth in vitro. More importantly, expression of chitinase and glucanase genes in different Trichoderma isolates was up-regulated, which could improve the biocontrol activity of T. harzianum. Therefore, here the authors envisage that combining biocontrol and biofumigation has the potential to provide sustainable and cost-effective strategies to manage soil-borne plant pathogens.

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Acknowledgements

The present study was undertaken as a part of the project entitled “Promoting IPM through a Common Minimum Programme in vegetable cultivation in Uttarakhand hills”, funded by Government of Uttarakhand (Letter No. 1757/Lekha-1/Ra-Kri. Yo./2008-09 dt. 10.07.2008). The authors are thankful to Government of Uttarakhand; Dean, College of Agriculture and Director Experiment Station, G.B.P.U.A.T., Pantnagar for their valuable assistance during this study.

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Authors and Affiliations

  1. Regional Station, ICAR-Indian Institute of Wheat and Barley Research, Shimla, H.P., 171002, India

    Pramod Prasad

  2. College of Agriculture, G.B. Pant University of Agriculture and Technology, U.S. Nagar, Pantnagar, Uttarakhand, 263 145, India

    J. Kumar

  3. Rain Forest Research Institute, Jorhat, Assam, 785010, India

    Shailesh Pandey

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  1. Pramod Prasad
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Correspondence to Pramod Prasad.

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Prasad, P., Kumar, J. & Pandey, S. Investigating Disease Controlling Ability of Brassica Volatiles and Their Compatibility with Trichoderma harzianum . Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 88, 887–896 (2018). https://doi.org/10.1007/s40011-016-0829-5

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  • DOI: https://doi.org/10.1007/s40011-016-0829-5

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