Abstract
A denitrifying biocathode in a microbial fuel cell was developed to investigate the replacement of the costly Pt-coated abiotic cathodes for electricity generation. The denitrifying biocathode was sandwiched between the dual-anode systems. The study investigated the performance for simultaneous treatment of wastewater on the anode, biological denitrification on the cathode and the potential recovery of electrical energy. Autotrophic biofilms performed denitrification on the cathode using supplied electrons by the biodegradation of organics on the anode. Graphite granules were used as electrodes for biofilm attachment, and nafion membranes were used as separators between electrodes. The system achieved a volumetric power of 7 ± 0.4 W m−3 net cathodic compartment (NCC) with the simultaneous removal of 229.5 ± 18 mg L−1 COD on anode and 88.9 g m−3 NCC day−1 nitrogen on cathode, respectively. The columbic efficiency for cathodic and anodic reactions was 98.9 ± 0.57 and 23.54 ± 0.87 %, respectively. This is a combined study for domestic wastewater treatment and biological denitrification in a compact MFC reactor. Further optimization of the system is desired to improve its performance and applicability.
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Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS (1979) Methanogens: reevaluation of a unique biological group. Microbiol Rev 43(2):260–296
Bard AJ, Faulkner LR (2001) Electrochemical methods: fundamentals and applications. Wiley, New York
Bergel A, Feron D, Mollica A (2005) Catalysis of oxygen reduction in PEM fuel cell by seawater biofilm. Electrochem Commun 7:900–904
Chaudhuri SK, Lovley DR (2003) Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nat Biotech 21:1229–1232
Clauwaert P, Rabaey K, Aelterman P, De Schamphelaire L, Pham HT, Boeckx P, Boon N, Verstraete W (2007a) Biological denitrification in microbial fuel cells. Environ Sci Technol 41(9):3354–3360
Clauwaert P, Van der Ha D, Boon N, Verbeken K, Verhaege M, Rabaey K, Verstraete W (2007b) Open-air biocathode enables effective electricity generation with microbial fuel cells. Environ Sci Technol 41(21):7564–7569
Collos Y, Mornet F, Sciandra A, Waser N, Larson A, Harrison PJ (1999) An optical method for the rapid measurement of micromolar concentrations of nitrate in marine phytoplankton cultures. J Appl Phycol 11:179–184
Donoso-Bravo A, Ruiz-Filippi G, Chamy R (2009) Anaerobic treatment of low-strength wastewater with a high fraction of particulate matter in an unconventional two-phase ASBRs system. Biochem Eng J 43:297–302
Freguia S, Rabaey K, Yuan Z, Keller J (2007) Electron and carbon balances in microbial fuel cells reveal temporary bacterial storage behavior during electricity generation. Environ Sci Technol 41(8):2915–2921
Freguia S, Rabaey K, Yuan ZG, Keller J (2008) Sequential anode-cathode configuration improves cathodic oxygen reduction and effluent quality of microbial fuel cells. Water Res 42:1387–1396
Gregory KB, Bond DR, Lovley DR (2004) Graphite electrodes as electron donors for anaerobic respiration. Environ Microbiol 6:596–604
Juang DF, Yang PC, Lee CH, Hsueh SC, Kuo TH (2011) Electrogenic capabilities of gram negative and gram positive bacteria in microbial fuel cell combined with biological wastewater treatment. Int J Environ Sci Technol 8(4):781–792
Juang DF, Yang PC, Kuo TH (2012) Effects of flow rate and chemical oxygen demand removal characteristics on power generation performance of microbial fuel cells. Int J Environ Sci Technol 9:267–280
Lefebvre O, Al-Mamun A, Ng HY (2008a) A microbial fuel cell equipped with a biocathode for organic removal and denitrification. Water Sci Technol 58(4):881–885
Lefebvre O, Al-Mamun A, Ooi WK, Tang Z, Chua DHC, Ng HY (2008b) An insight into cathode options for microbial fuel cells. Water Sci Technol 57(12):2031–2037
Lefebvre O, Ooi WK, Tang Z, Al-Mamun A, Chua D, Ng HY (2009) Optimization of a Pt-free cathode suitable for practical applications of microbial fuel cells. Biores Technol 100:4907–4910
Lefebvre O, Ha Nguyen TT, Al-Mamun A, Chang IS, Ng HY (2010) T-RFLP reveals high β-Proteobacteria diversity in microbial fuel cells enriched with domestic wastewater. J Appl Microbiol 109(3):839–850
Li W, Zhang S, Chen G, Hua Y (2014) Simultaneous electricity generation and pollutant removal in microbial fuel cell with denitrifying biocathode over nitrite. Appl Energy 126:136–141
Liu H, Logan BE (2004) Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. Environ Sci Technol 38(14):4040–4046
Liu H, Cheng S, Logan BE (2005) Production of electricity from acetate or butyrate in single chamber microbial fuel cell. Environ Sci Technol 39(2):658–662
Logan BE, Hamelers B, Rozenda R, Schroder U, Keller J, Freguia S, Aelterman P, Verstraete W, Rabaey K (2006) Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192
Logan BE, Cheng S, Valerie W, Garett E (2007) Graphite fiber brush anodes for increased power production in air cathode microbial fuel cells. Environ Sci Technol 41:3341–3346
Lovley DR (2006) Harvesting electricity with microorganisms. Natural Rev Microbiol 4:497–508
Lovley DR, Phillips EJP (1988) Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54(6):1472–1480
Lovley DR, Greening RC, Ferry JG (1984) Rapidly growing rumen methanogenic organism that synthesizes coenzyme-M and has a high affinity for formate. Appl Environ Microbiol 48(1):81–87
Min B, Logan BE (2004) Continuous electricity generation from domestic wastewater and organic substrates in a flat plate microbial fuel cell. Environ Sci Technol 38:5809–5814
Nasirahmadi S, Safekordi AA (2012) Enhanced electricity generation from whey wastewater using combinational cathodic electron acceptor in a two-chamber microbial fuel cell. Int J Environ Sci Technol 9:473–478
Park HI, Kim DK, Choi YJ, Pak D (2005) Nitrate reduction using an electrode as direct electron donor in a biofilm-electrode reactor. Process Biochem 40(10):3383–3388
Pynaert K, Smets BF, Beheydt D, Verstraete W (2004) Start-up of autotrophic nitrogen removal reactors via sequential biocatalyst addition. Environ Sci Tech 38:1228–1235
Rabaey K, Verstraete W (2005) Microbial fuel cells: novel biotechnology for energy generation. Trends Biotechnol 23:291–298
Rabaey K, Clauwaert P, Aelterman P, Verstraete W (2005) Tubular microbial fuel cells for efficient electricity generation. Environ Sci Technol 39:8077–8082
Rozendal RA, Hamelers HVM, Buisman CJN (2006) Effects of membrane cation transport on pH and microbial fuel cell performance. Environ Sci Technol 40(17):5206–5211
Rozendal RA, Hamelers HVM, Rabaey K, Keller J, Buisman CJN (2008) Towards practical implementation of bioelectrochemical wastewater treatment. Trends Biotechnol 26:450–459
TerHeijne A, Hamelers HVM, deWilde V, Rozendal RA, Buisman CJN (2006) A bipolar membrane combined with ferric iron reduction as an efficient cathode system in microbial fuel cells. Environ Sci Technol 40:5200–5205
Virdis B, Rabaey K, Yuan Z, Keller J (2008) Microbial fuel cells for simultaneous carbon and nitrogen removal. Water Res 42(12):3013–3024
Virdis B, Rabaey K, Rozendal RA, Yuan Z, Keller J (2010) Simultaneous nitrification, denitrification and carbon removal in microbial fuel cells. Water Res 44(9):2970–2980
Xie S, Liang P, Chen Y, Xia X, Huang X (2011) Simultaneous carbon and nitrogen removal using an oxic/anoxic-biocathode microbial fuel cells coupled system. Biores Technol 102:348–354
Zhang F, Zhen H (2012) Integrated organic and nitrogen removal with electricity generation in a tubular dual-cathode microbial fuel cell. Process Biochem 47(12):2146–2151
Zhang G, Zhang H, Zhang C, Zhang G, Yang F, Yuan G, Gao F (2013) Simultaneous nitrogen and carbon removal in a single chamber microbial fuel cell with a rotating biocathode. Process Biochem 48:893–900
Zhao F, Harnisch F, Schroder U, Scholz F, Bogdanoff P, Herrmann I (2005) Application of pyrolysed iron(II) phthalocyanine and CoTMPP based oxygen reduction catalysts as cathode materials in microbial fuel cells. Electrochem Commun 7:1405–1410
Zhen H, Largus TA (2006) Application of bacterial bio-cathodes in microbial fuel cells. Environ Sci Tech 40:5212–5214
Acknowledgments
Our sincere gratitude to Mr. S.G. Chandrasegaran for his technical assistance during fabrication of microbial fuel cells used in this study. Thanks to Miss Tan Hwee Bee, Miss Lee Leng Leng and Miss Tan Xiaolan to assist us in operating the equipments used for different analysis. This work was supported by a Grant from the Environment & Water and Industry Development Council, Singapore (MEWR 651/06/159).
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Al-Mamun, A., Lefebvre, O., Baawain, M.S. et al. A sandwiched denitrifying biocathode in a microbial fuel cell for electricity generation and waste minimization. Int. J. Environ. Sci. Technol. 13, 1055–1064 (2016). https://doi.org/10.1007/s13762-016-0943-1
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DOI: https://doi.org/10.1007/s13762-016-0943-1