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Aberrant expression of miR-199a in newborns with hypoxic-ischemic encephalopathy and its diagnostic and prognostic significance when combined with S100B and NSE

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Abstract

Neonatal hypoxic-ischemic encephalopathy (HIE) is a disorder mainly due to asphyxia during the perinatal period, and late diagnosis leads to high mortality. In this study, the expression of microRNA-199a (miR-199a) in HIE newborns was investigated, as well as its clinical significance in HIE diagnosis and prognosis. Circulating levels of S100B and NSE in HIE newborns were measured using enzyme-linked immunosorbent assay, and the expression of miR-199a was analyzed using quantitative real-time PCR. The diagnostic value of miR-199a, S100B and NSE was evaluated using the receiver operating characteristic (ROC) analysis, and their prognostic value was assessed by the evaluation of Gesell intellectual development of the HIE newborns. HIE newborns possessed significantly increased levels of S100B and NSE and decreased miR-199a (all P < 0.01). The Neonatal Behavioral Neurological Assessment (NBNA) score of HIE newborns was negatively correlated with S100B and NSE, while was positively correlated miR-199a. The ROC analysis results showed the diagnostic value of serum miR-199a, and the combined detection of miR-199a, S100B and NSE could obtained the highest diagnostic accuracy in HIE newborns. miR-199a expression was lowest in newborns with severe HIE, and it had diagnostic potential to distinguish HIE cases with different severity. Regarding the prognosis of neonatal HIE, the correlation of miR-199a, S100B, NSE with Gesell intellectual development was found in HIE newborns. The decreased miR-199a in HIE newborns serves as a potential diagnostic biomarker and may help to improve the diagnostic and prognostic value of S100B and NSE in neonatal HIE.

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Data availability

All data analyzed in this study are included in the manuscript.

Abbreviations

HIE:

Hypoxic-ischemic encephalopathy

miR-199a:

MicroRNA-199a

ROC:

Receiver operating characteristic

NBNA:

Neonatal Behavioral Neurological Assessment

CT:

Computed tomography

MRI:

Magnetic resonance imaging

NSE:

Neuron-specific enolase

miRNAs:

MicroRNAs

ELISA:

Enzyme-linked immunosorbent assay

qRT-PCR:

Quantitative real-time PCR

DQs:

Development quotients

AUC:

Area under the curve

BBB:

Blood brain barrier

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Authors and Affiliations

  1. Department of Neonatology, Women and Children’s Health Care Hospital of Linyi, Linyi, 276001, Shandong, China

    Min Li & Mei Ye

  2. Department of Pediatrics, Women and Children’s Health Care Hospital of Linyi, No.1, Qinghe South Road, Linyi, 276001, Shandong, China

    Guangyun Zhang

Authors
  1. Min Li
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  2. Mei Ye
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Contributions

ML and GZ designed the study, analyzed the clinical data and wrote the manuscript. MY collected the clinical data of the infants.

Corresponding author

Correspondence to Guangyun Zhang.

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The authors declare that they have no competing interests.

Ethics approval and consent to participate

The protocols of this study were approved by the Ethics Committee of Maternal and Child Health Hospital of Linyi and written informed consent was obtained from each infant’s parents.

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The consent for publication was obtained.

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Li, M., Ye, M. & Zhang, G. Aberrant expression of miR-199a in newborns with hypoxic-ischemic encephalopathy and its diagnostic and prognostic significance when combined with S100B and NSE. Acta Neurol Belg 121, 707–714 (2021). https://doi.org/10.1007/s13760-020-01408-0

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  • DOI: https://doi.org/10.1007/s13760-020-01408-0

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