Skip to main content

Advertisement

SpringerLink
Log in

Enzymatic protein hydrolysates and ultrafiltered peptide fractions from Cowpea Vigna unguiculata L bean with in vitro antidiabetic potential

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

Diabetes Mellitus (DM) is a disease that affects an increasing number of people worldwide consisting on a rise in blood sugars levels. Diabetes mellitus type 2 (DMT2) is the most common and accounts for 95% of cases. In this work, the antidiabetic potential of Cowpea (Vigna unguiculata L.) protein hydrolysates (PH) and ultrafiltered peptide fractions (UFPF) by means of the in vitro inhibition of the α-amylase, α-glucosidase, and dipeptidyl peptidase IV (DPP-IV) enzymes were analyzed. Their cytotoxicity was also assessed in healthy Vero cells lines to assess their safety as possible ingredients in food. The Cowpea proteins were hydrolyzed with Alcalase®-Flavourzyme® sequential system. Their highest inhibitory activities were: F > 10 kDa to inhibit α-amylase, F > 10 kDa and PH to inhibit α-glucosidase, and PH to inhibit DPP-IV. Their IC50 values were 31.58, 0.633, 1.81, and 2.06 mg protein per mL, respectively. In a second experiment, PHs and UFPFs were hydrolyzed with a pepsin–pancreatin sequential system and showed the following inhibitory activities: F1–3 kDa and F < 1 kDa to inhibit α-amylase, and F < 1 kDa to inhibit α-glucosidase. Their IC50 values were 65.79, 40.17, and 189.04 mg protein per mL, respectively. The degree of hydrolysis was extensive for all the PHs and the highest was obtained using the Alcalase®-Flavourzyme® sequential enzyme system. The PHs and UFPFs with the highest inhibitory activity of the α-amylase, α-glucosidase, and dipeptidyl DPP-IV enzymes did not show in vitro cytotoxicity activity in Vero Cells. Therefore, they are potential ingredients to produce functional food for patients with DMT2.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. IDF, Diabetes atlas de la FID, 8a edn. (International Diabetes Federation, Mariakerke,Belgium, 2017)

    Google Scholar 

  2. L. Xu, Y. Li, Y. Dai, J. Peng, Pharmacol. Res. 130, 451–465 (2018)

    Article  CAS  PubMed  Google Scholar 

  3. O. Power, A.B. Nongonierma, P. Jakeman, R.J. FitzGerald, Proc. Nutr. Soc. 73, 34–46 (2014)

    Article  PubMed  Google Scholar 

  4. J.M. Awika, K.G. Duodu, J. Funct. Foods 38, 686–697 (2017)

    Article  CAS  Google Scholar 

  5. M.R. Segura-Campos, L.A. Chel-Guerrero, D.A. Betancur-Ancona, J. Sci. Food Agric. 90, 2512–2518 (2010)

    Article  CAS  PubMed  Google Scholar 

  6. B. Grube, W.F. Chong, P.W. Chong, L. Riede, Obesity 22, 645–651 (2014)

    Article  PubMed  Google Scholar 

  7. L. Chel-Guerrero, V. Pérez-Flores, D. Betancur-Ancona, G. Dávila-Ortiz, J. Agric. Food Chem. 50, 584–591 (2002)

    Article  CAS  PubMed  Google Scholar 

  8. S. Kalra, J. Pak. Med. Assoc. 64, 474–476 (2014)

    PubMed  Google Scholar 

  9. P.M. Nielsen, D. Petersen, C. Dambmann, J. Food Sci. 66, 642–646 (2001)

    Article  CAS  Google Scholar 

  10. M.J. Cho, N. Unklesbay, F.H. Hsieh, A.D. Clarke, J. Agric. Food Chem. 52, 5895–5901 (2004)

    Article  CAS  PubMed  Google Scholar 

  11. L. Mojica, K. Chen, E.G. de Mejía, J. Food Sci. 80, H188–H198 (2015)

    Article  CAS  PubMed  Google Scholar 

  12. D.B.A. Mitra, M. Manjunatha, Int. J. Adv. Pharm. Sci. 1, 75–85 (2010)

    Google Scholar 

  13. B. Ahrén, A. Schweizer, S. Dejager, E.B. Villhauer, B.E. Dunning, J.E. Foley, Diabetes Obes Metab 13, 775–783 (2011)

    Article  PubMed  Google Scholar 

  14. M.M. Yust, J. Pedroche, J. Girón-Calle, J. Vioque, F. Millán, M. Alaiz, Food Chem. 85, 317–320 (2004)

    Article  CAS  Google Scholar 

  15. I. Camargo Filho, D.A.G. Cortez, T. Ueda-Nakamura, C.V. Nakamura, B. P. Dias Filho, Phytomedicine 15, 202–208 (2008)

    Article  CAS  PubMed  Google Scholar 

  16. M.R. Segura-Campos, L.A. Chel-Guerrero, D. A. Betancur-Ancona. Process Biochem. 46, 864–872 (2011)

    Article  CAS  Google Scholar 

  17. J. Ruiz-Ruiz, G. Dávila-Ortíz, L. Chel-Guerrero, D. Betancur-Ancona, J. Food Biochem. 37, 26–35 (2013)

    Article  CAS  Google Scholar 

  18. E. Polanco-Lugo, G. Davila-Ortiz, D.A. Betancur-Ancona, L.A. Chel-Guerrero, Food Sci. Technol. 34, 441–448 (2014)

    Article  Google Scholar 

  19. J. Torruco-Uco, L. Chel-Guerrero, A. Martínez-Ayala, G. Dávila-Ortíz, D. Betancur-Ancona, LWT Food Sci. Technol. 42, 1597–1604 (2009)

    Article  CAS  Google Scholar 

  20. M.E. Oseguera-Toledo, E. Gonzalez de Mejia, S.L. Amaya-Llano, Food Res. Int. 76, 839–851 (2015)

    Article  CAS  PubMed  Google Scholar 

  21. M. Segura-Campos, J. Ruiz-Ruiz, L. Chel-Guerrero, D. Betancur-Ancona, CYTA J. Food 11, 208–215 (2013)

    Article  CAS  Google Scholar 

  22. P.V. Sandoval, Doctoral Thesis, Universidad Autónoma de Yucatán (2015)

  23. Y.Y. Ngoh, T.S. Lim, C.Y. Gan, Enzyme Microb. Technol. 89, 76–84 (2016)

    Article  CAS  PubMed  Google Scholar 

  24. Y.Y. Ngoh, C.Y. Gan, Food Chem. 190, 331–337 (2016)

    Article  CAS  PubMed  Google Scholar 

  25. H.J. Lee, H.S. Lee, J.W. Choi, K.S. Ra, J.M. Kim, H.J. Suh, J. Agric. Food Chem. 59, 11522–11525 (2011)

    Article  CAS  PubMed  Google Scholar 

  26. I.M.E. Lacroix, E.C.Y. Li-Chan, J. Agric. Food Chem. 61, 7500–7506 (2013)

    Article  CAS  PubMed  Google Scholar 

  27. A.B. Nongonierma, R.J. Fitzgerald, Peptides 39, 157–163 (2013)

    Article  CAS  PubMed  Google Scholar 

  28. T. de Souza Rocha, L.M.R. Hernandez, Y.K. Chang, E.G. de Mejía, Food Res. Int. 64, 799–809 (2014)

    Article  CAS  PubMed  Google Scholar 

  29. Y. Ren, K. Liang, Y. Jin, M. Zhang, Y. Chen, H. Wu, F. Lai, J. Funct. Foods 26, 439–450 (2016)

    Article  CAS  Google Scholar 

  30. M.E. Oseguera-Toledo, E. de Mejía, R. Reynoso-Camacho, A. Cardador-Martínez, S.L. Amaya-Llano, Nutrafoods 13, 147–157 (2014)

    Article  CAS  Google Scholar 

  31. R. Mora-Escobedo, MdelC. Robles-Ramírez, E. Ramón-Gallegos, R. Reza-Alemán, Plant Foods Hum. Nutr. 64, 271–278 (2009)

    Article  CAS  PubMed  Google Scholar 

  32. A.J. Velarde-Salcedo, A. Barrera-Pacheco, S. Lara-González, A. Montero-Morán, A. Díaz-Gois, E.G. de Mejía, A.P. Barba de la Rosa. Food Chem. 136, 758–764 (2013)

    Article  CAS  PubMed  Google Scholar 

  33. S. Lorey et al., Eur. J. Biochem. 270, 2147–2156 (2003)

    Article  CAS  PubMed  Google Scholar 

  34. M.G. Kang, S.H. Yi, J.S. Lee, Mycobiology 41, 149–154 (2013)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. R.L. Freitas, A.R. Teixeira, R.B. Ferreira, J. Agric. Food Chem. 52, 1682–1687 (2004)

    Article  CAS  PubMed  Google Scholar 

  36. Z. Yu, Y. Yin, W. Zhao, J. Liu, F. Chen, Food Chem. 135, 2078–2085 (2012)

    Article  CAS  PubMed  Google Scholar 

  37. J. Girón-Calle, M. Alaiz, J. Vioque, Food Res. Int. 43, 1365–1370 (2010)

    Article  CAS  Google Scholar 

  38. P.H.K. Ngai, T.B. Ng, Peptides 25, 2063–2068 (2004)

    Article  CAS  PubMed  Google Scholar 

  39. H.W. Jack, B.N. Tzi, Int. J. Biochem. Cell Biol. 37, 1626–1632 (2005)

    Article  CAS  Google Scholar 

  40. N.M. Ali, S.K. Yeap, H.M. Yusof, B.K. Beh, W.Y. Ho, S.P. Koh, M.P. Abdullah, N.B. Alitheen, K. Long, J. Sci. Food Agric. 96, 1648–1658 (2016)

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors thank the Consejo Nacional de Ciencia y Tecnología (CONACYT-México) and Red Temática de Farmoquímicos for their Project support to this publication. CONACYT is acknowledged for providing the MSc scholarship for the K. Jiménez and Fundación IMSS for research grant to R. Moo.

Author information

Authors and Affiliations

  1. Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Chuburná de Hidalgo Inn, Periférico Norte Kilómetro 33.5, Tablaje Catastral 13615, CP 97203, Mérida, Yucatán, México

    Eduardo Castañeda-Pérez, Karina Jiménez-Morales, Luis Chel-Guerrero & David Betancur-Ancona

  2. Unidad de Investigación Médica Yucatán, Unidad Médica de Alta Especialidad Hospital de Especialidades-1 Mérida, Instituto Mexicano del Seguro Social, Mérida, Yucatán, México

    Carlos Quintal-Novelo & Rosa Moo-Puc

Authors
  1. Eduardo Castañeda-Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karina Jiménez-Morales
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Carlos Quintal-Novelo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rosa Moo-Puc
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luis Chel-Guerrero
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. David Betancur-Ancona
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to David Betancur-Ancona.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Castañeda-Pérez, E., Jiménez-Morales, K., Quintal-Novelo, C. et al. Enzymatic protein hydrolysates and ultrafiltered peptide fractions from Cowpea Vigna unguiculata L bean with in vitro antidiabetic potential. J IRAN CHEM SOC 16, 1773–1781 (2019). https://doi.org/10.1007/s13738-019-01651-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-019-01651-0

Keywords

Search

Navigation