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Direct electron transfer of horseradish peroxidase at Co3O4–graphene nanocomposite modified electrode and electrocatalysis

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Abstract

In this paper, the mixture of Co3O4–graphene nanocomposite and horseradish peroxidase (HRP) was spread on the surface of carbon ionic liquid electrode (CILE). Then, Nafion film was used for the immobilization. The results of spectroscopy proved that HRP kept up its native structure in the complex material. Direct electrochemistry of HRP resulted in a couple of quasi-reversible redox waves on cyclic voltammograms, reflecting the realization of direct electron transfer of HRP with electrode. The improvement in electrochemical responses was due to the usage of highly conductive Co3O4–graphene nanocomposite with biocompatible interface. Electrochemical parameters such as the electron transfer coefficient (α) was estimated as 0.47, and the apparent heterogeneous electron transfer rate constant (k s) was calculated as 2.90 s−1. The HRP modified electrode exhibited good electrochemical catalytic ability toward the reduction of trichloroacetic acid and NaNO2. As a consequence, an updated third-generation electrochemical HRP biosensor with Co3O4–GR/CILE was constructed successfully.

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Acknowledgements

The financial support of this project by the National Natural Science Foundation of China (21365010, 21665007), the International Science and Technology Cooperation Project of Hainan Province (KJHZ2015-13), the Science and Research Key Project of Universities of Hainan Province (Hnky2016ZD-10).

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Authors and Affiliations

  1. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Wen Zheng, Wei Chen, Wenju Weng & Guangjiu Li

  2. College of Electromechanical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Lihua Liu

  3. College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, People’s Republic of China

    Jiewen Wang & Wei Sun

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  1. Wen Zheng
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  2. Wei Chen
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  3. Wenju Weng
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  4. Lihua Liu
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  5. Guangjiu Li
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  7. Wei Sun
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Correspondence to Wei Sun.

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Zheng, W., Chen, W., Weng, W. et al. Direct electron transfer of horseradish peroxidase at Co3O4–graphene nanocomposite modified electrode and electrocatalysis. J IRAN CHEM SOC 14, 925–932 (2017). https://doi.org/10.1007/s13738-016-1042-4

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