Abstract
DTIC or dacarbazine is a photosensitive drug which is analyzed by chromatography methods. In this study, chemometric methods were applied to resolve the UV–Vis absorbance spectra recorded during irradiation of DTIC by an artificial lighting source. The photo-degradation reaction was monitored at different pH media (between 2 and 12). Multivariate curve resolution-alternative least square was then employed to simultaneously analyze the absorbance data matrices obtained at various pH media. Factor analysis revealed the presence of five chemical species co-existing in the reaction media. Regarding the analysis of concentration profiles of the detected species at different pH values, three species were attributed to the different acid–base states of DTIC, while the remaining components were considered as acid–base states of the photoproduct. Eventually, the presence of major photoproduct (2-azahypoxanthine) was confirmed by the HPLC method.
Similar content being viewed by others
References
S.K. Carter, M.A. Friedman, Eur. J. Cancer 8, 85 (1972)
M. Slavik, Cancer Treat. Rep. 60, 213 (1976)
S.K. Carter, M. Slavik, Anu. Rev. Pharmacol. 14, 157 (1974)
G.M. Baird, M.L. Willoughby, Lancet 2, 681 (1978)
J.K. Horton, M.F. Stevens, J. Pharm. Pharmacol. 33, 808 (1981)
M. El Aatmani, S. Poujol, C. Astre, F. Malosse, F. Pinguet, Am. J. Health Syst. Pharm. 59, 1351 (2002)
B.V. Shetty, R.L. Schowen, M. Slavik, C.M. Riley, J. Pharm. Biomed. Anal. 10, 675 (1992)
H.H. Tonnesen, Photostability of Drugs and Drug Formulations, 2nd edn. (CRC Press, USA, 2004), pp. 41–66
British Pharmacopoeia. Vol. 1, (Great Britain, 2004)
The United States Pharmacopeia 28 : The national formulary 23. Vol. 1, (Webcom Limited, Canada, 2005) pp. 586–570
K. Javidnia, B. Hemmateenejad, R. Miri, M. Saeidi-Boroujeni, J. Pharm. Biomed. Anal. 46, 597 (2008)
G. Ragno, G. Ioele, M. De Luca, A. Garofalo, F. Grande, A. Risoli, J. Pharm. Biomed. Anal. 42, 39 (2006)
A. de Juan, M. Maeder, M. Martı́nez, R. Tauler. Chemometr. Intell. Lab. Syst. 54, 123 (2000)
A. de Juan, R. Tauler, Crit. Rev. Anal. Chem. 36, 163 (2006)
B. Hemmateenejad, K. Javidnia, M. Saeidi-Boroujeni, J. Pharm. Biomed. Anal. 47, 625 (2008)
S. Norman, M. Maeder, Criti. Rev. Anal. Chem. 36, 199 (2006)
M. Shamsipur, B. Hemmateenejad, M. Akhond, J. Solution Chem. 32, 819 (2003)
J.M. Amigo, A. de Juan, J. Coello, S. Maspoch, Anal. Chim. Acta 567, 245 (2006)
L. Blanchet, A. Mezzetti, C. Ruckebusch, J.-P. Huvenne, A. De Juan, Anal. Bioanal. Chem. 387, 1863 (2007)
J.C. Esteves da Silva, R. Tauler, Appl. Spectrosc. 60, 1315 (2006)
H.T.S. Britton, R.A. Robinson, J. Chem. Soc.,1456 (1931)
M. Davies, Analyst. 84, 248 (1959)
A. Albini, E. Fasani, Drugs: Photochemistry and Photostability;[based on the Proceedings of the 2nd International Meeting on Photostability of Drugs, (Royal Society of Chemistry, Pavia, 1998) pp.115–156
S. Mas, A. de Juan, S. Lacorte, R. Tauler, Anal. Chim. Acta 618, 18 (2008)
H. Gampp, M. Maeder, C.J. Meyer, A. Zuberbuhler, Chimia 39, 315 (1985)
M. Maeder, A. De Juan, Two-Way Data Analysis: Evolving Factor, Ed. by S.D. Brown, R. Tauler and B. Walczak (Elsevier, Oxford, 2009), Vol. 2
C. Ruckebusch, A. De Juan, L. Duponchel, J. Huvenne, Chemometr. Intell. Lab. Syst. 80, 209 (2006)
Acknowledgments
This manuscript is a part of the thesis of Sina Bahrpeyma and was supported financially by the Shiraz University of Medical Sciences Research Council. The authors would like to thank Dr. Shirin Tavakoli for the tremendous help with the script.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Bahrpeyma, S., Hemmateenejad, B. & Javidnia, K. Photo-degradation study of dacarbazine by spectrophotometric–chemometrics and HPLC methods. J IRAN CHEM SOC 13, 221–229 (2016). https://doi.org/10.1007/s13738-015-0729-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13738-015-0729-2