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β-Lactoglobulin nanoparticle as a chemotherapy agent carrier for oral drug delivery system

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Abstract

Today, fabrication of new materials in nano scale based on protein-polysaccharide for targeted drug delivery by oral way is unresolved question for researchers in drug delivery system field. In this work, nanoparticles were made by β-lactoglobulin (β-LG), as a globular milk whey protein, complex with low methoxyl pectin (LMP) for chemotherapy agent (oxali-palladium) delivery. Affinity of β-LG to chemotherapy agent was investigated based on the intrinsic fluorescence intensity at 25 °C, so that β-LG binds to chemotherapy agent with molar ratio of 1:1. Hence, the nanoparticles were synthesized at various pH at ambient temperature. The results indicated that the nanoparticles were fabricated smaller than 200 nm at optimal pH approximately close to isoelectric point of β-LG with stability and solubility properties at ambient temperature. Also, scanning electron microscopy results demonstrated that nanoparticles were formed spherical so that were homogeneously distributed. Moreover, the results of dissolution profiles of drug release from β-LG nanoparticle-LMP complex at 37 °C show that in acidic conditions, nanoparticles are stable and there is no significant drug release but in alkaline conditions drug is released from nanoparticles. Therefore, we suggest β-LG nanoparticles-LMP complex are biodegradable and biocompatible and might be useful as targeted oral drug delivery system.

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Abbreviations

β-LG:

Β-Lactoglobulin

LMP:

Low methoxyl pectin

DLS:

Dynamic light scattering

SEM:

Scanning electron microscopy

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Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Behafarid Ghalandari & Kazem Parivar

  2. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

    Adeleh Divsalar

  3. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

  4. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran

    Ali Akbar Saboury

Authors
  1. Behafarid Ghalandari
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  2. Adeleh Divsalar
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  3. Ali Akbar Saboury
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  4. Kazem Parivar
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Correspondence to Adeleh Divsalar.

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Ghalandari, B., Divsalar, A., Saboury, A.A. et al. β-Lactoglobulin nanoparticle as a chemotherapy agent carrier for oral drug delivery system. J IRAN CHEM SOC 12, 613–619 (2015). https://doi.org/10.1007/s13738-014-0519-2

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  • DOI: https://doi.org/10.1007/s13738-014-0519-2

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