Abstract
Natural products provide a promising source of efficient bioactive compounds against oxidative damage. However, the interactions between phytochemicals affect the antioxidant potency. The aim of the present study was to evaluate the in vitro antioxidant potency of twelve plants used in the central north of Morocco and to analyze the combined effect between the most active extracts. The main active plant extracts were subjected to binary and ternary combinations using the simplex-centroid design. Important synergetic effects were observed and the constrained mixture design allowed the optimization of the mixture presenting the highest antioxidant activity at 90.47%. The results from the present work showed that Moroccan plants such as Inula viscosa, Punica granatum and Crataegus oxyacantha could constitute a promising source for developing antioxidants. Moreover, the antioxidant potency can be enhanced using constrained mixture design.
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References
Al-Bayati FA (2008) Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. J Ethnopharmacol 116:403–406. https://doi.org/10.1016/j.jep.2007.12.003
AlBasher G, Abdel-Daim MM, Almeer R, Ibrahim KA, Hamza RZ, Bungau S, Aleya L (2020) Synergistic antioxidant effects of resveratrol and curcumin against fipronil-triggered oxidative damage in male albino rats. Environ Sc Pollut Res 27:6505–6514. https://doi.org/10.1007/s11356-019-07344-8
Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N (2019) Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants. https://doi.org/10.3390/plants8040096
Bag A, Chattopadhyay RR (2015) Evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination. PLoS ONE 10:1–17. https://doi.org/10.1371/journal.pone.0131321
Baj T, Baryluk A, Sieniawska E (2018) Industrial crops & products application of mixture design for optimum antioxidant activity of mixtures of essential oils from Ocimum basilicum L., Origanum majorana L. and Rosmarinus officinalis L. Ind Crop Prod 115:52–61. https://doi.org/10.1016/j.indcrop.2018.02.006
Benabderrahmane W, Lores M, Benaissa O, Lamas JP, de Miguel T, Amrani A, Benayache F, Benayache S (2019) Polyphenolic content and bioactivities of Crataegus oxyacantha L. (Rosaceae). Nat Prod Res 35(4):627–632. https://doi.org/10.1080/14786419.2019.1582044
Bezerra MA, Castro JT, Macedo RC, Da Silva DG (2010) Use of constrained mixture design for optimization of method for determination of zinc and manganese in tea leaves employing slurry sampling. Anal Chim Acta 670:33–38. https://doi.org/10.1016/j.aca.2010.04.063
Butterfield DA, Halliwell B (2019) Oxidative stress, dysfunctional glucose metabolism and Alzheimer disease. Nat Rev Neurosci 20:148–160. https://doi.org/10.1038/s41583-019-0132-6
Da Silva AP, Rocha R, Silva CML, Mira L, Filomena Duarte M, Helena Florncio M (2000) Antioxidants in medicinal plant extracts. A research study of the antioxidant capacity of Crataegus. Hamamelis and Hydrastis Phytother Res 14:612–616. https://doi.org/10.1002/1099-1573(200012)14:8%3c612:AID-PTR677%3e3.0.CO;2-T
Güder A, Korkmaz H (2012) Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iran J Pharmac Res 11:913–923. https://doi.org/10.22037/ijpr.2012.1159
Heo BG, Park YS, Chon SU, Lee SY, Cho JY, Gorinstein S (2007) Antioxidant activity and cytotoxicity of methanol extracts from aerial parts of Korean salad plants. BioFactors 30:79–89. https://doi.org/10.1002/biof.5520300202
Hidalgo M, Sánchez-Moreno C, de Pascual-Teresa S (2010) Flavonoid-flavonoid interaction and its effect on their antioxidant activity. Food Chem 121:691–696. https://doi.org/10.1016/j.foodchem.2009.12.097
Kheyar-Kraouche N, da Silva AB, Serra AT, Bedjou F, Bronze MR (2018) Characterization by liquid chromatography–mass spectrometry and antioxidant activity of an ethanolic extract of Inula viscosa leaves. J Pharmac Biomed Anal 156:297–306. https://doi.org/10.1016/j.jpba.2018.04.047
Liu RH (2003) Health benefits of fruit and vegetables are from additive and. The American J Clinic Nutr 78:3–6
Lonni AASG, Longhini R, Lopes GC, De Mello JCP, Scarminio IS (2012) Statistical mixture design selective extraction of compounds with antioxidant activity and total polyphenol content from Trichilia catigua. Anal Chim Acta 719:57–60. https://doi.org/10.1016/j.aca.2011.12.053
Maia ECR, Borsato D, Moreira I, Spacino KR, Rodrigues PRP, Gallina AL (2011) Study of the biodiesel B100 oxidative stability in mixture with antioxidants. Process Technol 92:1750–1755. https://doi.org/10.1016/j.fuproc.2011.04.028
Marcheafave GG, Tormena CD, Pauli ED, Rakocevic M, Bruns RE, Scarminio IS (2019) Experimental mixture design solvent effects on pigment extraction and antioxidant activity from Coffea arabica L. leaves. Microchem J 146:713–721. https://doi.org/10.1016/j.microc.2019.01.073
Martinez-Gomez A, Caballero I, Blanco CA (2020) Phenols and melanoidins as natural antioxidants in beer. Structure, reactivity and antioxidant activity. Biomol. https://doi.org/10.3390/biom10030400
Myers RH, Khuri A, Carter WH (1989) Response surface methodology: 1966–l988. Technometrics 31:137–157. https://doi.org/10.1080/00401706.1989.10488509
Newman DJ, Cragg GM (2016) Natural Products as Sources of New Drugs from 1981 to 2014. J Nat Prod 79:629–661. https://doi.org/10.1021/acs.jnatprod.5b01055
Oudane B, Boudemagh D, Bounekhel M, Sobhi W, Vidal M, Broussy S (2018) Isolation, characterization, antioxidant activity, and protein-precipitating capacity of the hydrolyzable tannin punicalagin from pomegranate yellow peel (Punica granatum). J Mol Struc 1156:390–396. https://doi.org/10.1016/j.molstruc.2017.11.129
Ouedrhiri W, Balouiri M, Bouhdid S, Moja S, Chahdi FO, Taleb M, Greche H (2016) Mixture design of Origanum compactum, Origanum majorana and Thymus serpyllum essential oils: Optimization of their antibacterial effect. Ind Crop Prod 89:1–9. https://doi.org/10.1016/j.indcrop.2016.04.049
Ouedrhiri W, Bouhdid S, Balouiri M, El Ouali LA, Moja S, Ouazzani Chahdi F, Greche H (2015) Chemical composition of Citrus aurantium L., leaves and zest essential oils, their antioxidant, antibacterial single and combined effects. J Chem Pharmac Res 7:78–84
Pelissari FM, Yamashita F, Garcia MA, Martino MN, Zaritzky NE, Grossmann MVE (2012) Constrained mixture design applied to the development of cassava starch-chitosan blown films. J Food Eng 108:262–267. https://doi.org/10.1016/j.jfoodeng.2011.09.004
Peoples JN, Saraf A, Ghazal N, Pham TT, Kwong JQ (2019) Mitochondrial dysfunction and oxidative stress in heart disease. Exp Mol Med. https://doi.org/10.1038/s12276-019-0355-7
Pinelo M, Manzocco L, Nuñez MJ, Nicoli MC (2004) Interaction among Phenols in Food Fortification: Negative Synergism on Antioxidant Capacity. J Agr Food Chem 52:1177–1180. https://doi.org/10.1021/jf0350515
Ran L, Chi Y, Huang Y, He Q, Ren Y (2020) Synergistic antioxidant effect of glutathione and edible phenolic acids and improvement of the activity protection by coencapsulation into chitosan-coated liposomes. Lwt 127:109409. https://doi.org/10.1016/j.lwt.2020.109409
Rezayian M, Niknam V, Ebrahimzadeh H (2019) Oxidative damage and antioxidative system in algae. Toxicol Rep 6:1309–1313. https://doi.org/10.1016/j.toxrep.2019.10.001
Rossetto M, Vanzani P, Mattivi F, Lunelli M, Scarpa M, Rigo A (2002) Synergistic antioxidant effect of catechin and malvidin 3-glucoside on free radical-initiated peroxidation of linoleic acid in micelles. Arch Biochem Biophy 408:239–245. https://doi.org/10.1016/S0003-9861(02)00561-1
Scheffé H (1963) The Simplex-Centroid Design for Experiments with Mixtures. J R Sta Soc B 25:235–251. https://doi.org/10.1111/j.2517-6161.1963.tb00506.x
Seibert JB, Bautista-Silva JP, Amparo TR, Petit A, Pervier P, dos Santos Almeida JC, Azevedo MC, Silveira BM, Brandão GC, de Souza GHB, de Medeiros Teixeira LF, dos Santos ODH (2019) Development of propolis nanoemulsion with antioxidant and antimicrobial activity for use as a potential natural preservative. Food Chem 287:61–67. https://doi.org/10.1016/j.foodchem.2019.02.078
Zeouk I, Ouali Lalami A, El Bekhti K (2019) In vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: a Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus. Asian J Pharmac Clin Res 12:285–292. https://doi.org/10.22159/ajpcr.2019.v12i3.30395
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Ikrame Zeouk has no conflict of interest. Wessal Ouedrhiri has no conflict of interest. Chaimae Rais has no conflict of interest. Chaimae Slimani has no conflict of interest. Khadija Bekhti has no conflict of interest.
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Zeouk, I., Ouedrhiri, W., Rais, C. et al. Phytochemical quantification and optimization of synergistic antioxidant potency of Moroccan medicinal plants using constrained mixture design. ADV TRADIT MED (ADTM) (2024). https://doi.org/10.1007/s13596-024-00754-1
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DOI: https://doi.org/10.1007/s13596-024-00754-1