Abstract
A conventional plant flavonoid known as myricetin is well-known for its wide range of effects, including its strong anti-inflammatory, anti-cancer, and antioxidant characteristics. In this study, we examined anti-inflammatory potential of Myricetin (MN) by looking at the expressions of COX-2, IL-6 and TNF-α in leukemia cancer cell lines (THP-1). The THP-1 was cultured in Roswell Park Memorial Institute medium supplemented with 10% foetal bovine serum and penicillin (100 U/ml) for the purposes of the in-vitro investigations. Cell viability and cytotoxicity testing were done using the MTT assay. To prepare the ligand and proteins for the in-silico studies, The structures of the targeted anti-inflammatory proteins COX-2 (PDB ID: 5KIR), TNF- (PDB ID: 1TNF), and IL-6 (PDB ID: 1ALU) were retrieved from Protein Data Bank. Furthermore, docking experiments were carried out using the AutoDock Vina software. In comparison to the reference drug cisplatin (9.46 µg/ml) the IC50 value for MN was found to be 90.161 µg/ml. The cells pretreated with MN showed a significantly lower expression of anti-inflammatory components (COX-2, TNF-α and IL6) upon measured by geometric mean fluorescence intensity as compared to the LPS-induced control cells. Additionally, MN has shown a strong interaction with COX-2, TNF-α and IL-6 in simulation studies. To conclude, MN exhibits a potent anti-inflammatory effect both in-silico and in-vitro studies. Further, in vivo investigations are needed to gain more comprehensive understanding of the molecule.
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Acknowledgements
Authors acknowledge the facilities provided by Maharani Lakshmi Ammanni College For Women (mLAC). The partial work was carried out in DST-FIST for PG Level O facility (SR/FST/College-2018-404) and DBT-BIF facility at mLAC.
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This is the PhD work of KN. KN conducted the work and wrote the manuscript, KN. and TU provided analysis support, RN. Supported with wet laboratory work and data analysis, HPP supported with review and corrections, SKM and NR conceptualised the projects, SKM also arranged the funding and all authors reviewed the manuscript.
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Khushbu Nishad has no conflict of interest. Talambedu Usha has no conflict of interest. H. P. Prashanth Kumar has no conflict of interest. Nijalingappa Ramesh has no conflict of interest. Sushil Kumar Middha has no conflict of interest.
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Nishad, K., Usha, T., Kumar, H.P.P. et al. Anti-inflammatory potential of myricetin in leukemia cells: in silico and in vitro exploration. ADV TRADIT MED (ADTM) (2024). https://doi.org/10.1007/s13596-023-00740-z
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DOI: https://doi.org/10.1007/s13596-023-00740-z