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Assessment of wound healing activity of ethanolic extracts of Pistacia lentiscus L. leaves and Quercus ilex L. bark in full thickness skin excision in rats

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Abstract

The objective of our study was to evaluate the wound healing effect of ethanolic extracts of pistachio (Pistacia lentiscus L.) leaves and holm oak (Quercus ilex L.) bark on full-thickness excisions (4 cm2) in Wistar rats. Forty-two rats were divided into seven equal groups that received different treatments; two groups were treated with Quercus ilex bark extract (10% and 30%), two other groups with Pistacia lentiscus leaves extract (10% and 30%), one group received Cicatryl-bio® and considered as a positive control, a group treated with pure Petroleum jelly, and the last was left as an untreated negative control. The evaluation of the excision zones was estimated by a planimetric study using an image processing software (ImageJ®) and histological examination of healed skin tissue. The results revealed that Pistacia lentiscus leaves and Quercus ilex bark promote wound healing effect because they accelerated wound contraction and reduced epithelialization period. Ointments prepared from Quercus ilex bark and Pistacia lentiscus leaves ethanolic extracts exhibited effective wound healing action in Wistar rat’s skin excisions. Both preparations showed an accelerated healing process compared to the other formulations. To the best of the author's knowledge, this is the first study of wound healing effect of pistachio (Pistacia lentiscus) leaves and holm oak (Quercus ilex) bark.

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Acknowledgements

The authors are thankful and acknowledge the support of Mrs. Boudali Soumia from the Histopathology Laboratory at the Veterinary Sciences Institute, University of Tiaret.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Biomedicine, Veterinary Sciences Institute, University of Tiaret, 14000, Tiaret, Algeria

    Houari Hemida

  2. Department of Nature and Life Sciences, Faculty of Nature and Life Sciences, University of Tiaret, 14000, Tiaret, Algeria

    Koula Doukani, Abdelkader Zitouni, Brahim Miloud, Houcine Beggar & Hasna Bouhenni

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  1. Houari Hemida
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  2. Koula Doukani
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  3. Abdelkader Zitouni
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  4. Brahim Miloud
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Correspondence to Koula Doukani.

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Ethical statement

This assay was conducted with accordance with the European Communities Council Directive (2010/63/EU) for animal experiments. The experimental study took place in the animal house of the Veterinary Institute-Tiaret, and the experimental protocol was designed and followed by Veterinarians. Authors are members of the Algerian Association of Sciences in Animal Experimentation (AASEA) (Agreement Number: 45/DGLPAG/DVA.SDA.14).

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Hemida, H., Doukani, K., Zitouni, A. et al. Assessment of wound healing activity of ethanolic extracts of Pistacia lentiscus L. leaves and Quercus ilex L. bark in full thickness skin excision in rats. ADV TRADIT MED (ADTM) 22, 589–597 (2022). https://doi.org/10.1007/s13596-021-00557-8

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  • DOI: https://doi.org/10.1007/s13596-021-00557-8

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