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Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata

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Abstract

This study is devoted to the estimation of total bioactive contents and the evaluation of acute toxicity and in vivo anti-inflammatory effects and the assessment of in vitro antioxidant and anti-arthritic potential of the species Scabiosa stellata. The anti-arthritic activity was performed by bovine serum albumin denaturation method and the antioxidant activity was evaluated by eight different methods. Furthermore, the acute oral toxicity and the anti-inflammarory activity using the model of carrageenan-induced paw edema were tested on male Wistar rats. The ethyl acetate extract recorded the highest content of polyphenols, flavonoids and tannins. All the crude extracts (PE, EtAOc and n-BuOH) had antioxidant activities in various assays and prevent the denaturation of bovin serum albumin in dose depending manner. The oral administration of the ethyl acetate extract at various concentrations (500, 1000 and 2000 mg/kg), does not induce any toxic symptoms or mortalities in all the treated animals. No significant changes in biochemical and hematological parameters compared to the control group were observed. Thus, the approximate acute lethal dose 50 of this extract was higher than 2000 mg/kg. In addition, the administration of ethyl acetate extract at doses of 50 and 75 mg/kg prevents a significant acute paw edema and present a higher anti-inflammatory activity than Diclofenac as a positive control.

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Acknowledgements

The authors wish to express thanks to the DGRSDT of the Algerian ministry of higher education and scientific research for providing a research grant (Project 8/u05/853).

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Authors and Affiliations

  1. Laboratory of Chemistry of Materials and Living: Activity and Reactivity (LCMVAR), University of Batna-1, Batna, Algeria

    Chaima Mouffouk & Leila Hambaba

  2. Laboratory of Biotechnology of Bioactive Molecules and Cell Physiopathology, University of Batna-2, Batna, Algeria

    Chaima Mouffouk, Leila Hambaba, Sara mouffouk & Messaoud Hachemi

  3. Laboratoire de Chimie et Chimie de l’Environnement (L.C.C.E), Département de Chimie, Faculté des Sciences de la Matière, Université de Batna-1, Batna, Algeria

    Hamada Haba & Soumia Mouffouk

  4. Centre de Recherche en Biotechnologie, Ali Mendjli Nouvelle Ville UV 03, BP E73, Constantine, Algeria

    Chawki Bensouici

  5. Laboratoire d’Anapathologie Centre Anticancéreux (CAC) Batna, Département de médecine, Université de Batna-2, Batna, Algeria

    Hachani Khadraoui

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  1. Chaima Mouffouk
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  2. Leila Hambaba
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  3. Hamada Haba
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  6. Sara mouffouk
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Correspondence to Hamada Haba.

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The experimental protocol was approved by the Biology Discipline, Life Science of University of Batna-2, Algeria.

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Mouffouk, C., Hambaba, L., Haba, H. et al. Acute toxicity and in vivo anti-inflammatory effects and in vitro antioxidant and anti-arthritic potential of Scabiosa stellata. Orient Pharm Exp Med 18, 335–348 (2018). https://doi.org/10.1007/s13596-018-0320-3

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  • DOI: https://doi.org/10.1007/s13596-018-0320-3

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