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Analgesic and anti-inflammatory activities of methanolic extract of Mallotus repandus stem in animal models

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Abstract

The aim of our current study is to investigate the analgesic and anti-inflammatory potentials of methanolic extract of Mallotus repandus stem (MSM) using different in vivo assay models. Analgesic and anti-inflammatory activities were evaluated using acetic acid induced writhing test, tail immersion method, hot-plate test, formalin induced hind paw licking, xylene induced ear edema, carrageenan-induced paw edema and cotton pellet induced granuloma in animal model at doses of 500, 1000 and 2000 mg/kg body weight. MSM showed significant (p < 0.05) analgesic activities in the acetic acid-induced writhing tests in mice. In the tail immersion and hot-plate test, MSM significantly prolonged the latency period (p < 0.05). Significant (p < 0.05) formalin induced paw licking inhibition was observed at different doses and the highest 74.47% inhibition was observed at 2000 mg/kg. Besides, MSM showed significant (p < 0.05) anti-inflammatory responses in carrageenan induced paw edema, xylene induced ear edema and cotton pellet induced granuloma methods. These findings suggest that the plant may be a potential source for the development of new analgesic and anti-inflammatory agent.

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Acknowledgements

The authors are thankful to Focused Research on Ayurvedic Medicine and Education (F.R.A.M.E) Laboratory, Department of Pharmacy and all faculty members and the technical staffs of the Department of Pharmacy, Jahangirnagar University for their kind co-operation. We would express our special thanks to Mr. Shafiqul Islam for ensuring a constant supply of animals followed by proper maintenance and care of these animals during all throughout the experimental period.

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Authors and Affiliations

  1. Department of Pharmacy, Jahangirnagar University, Savar, Dhaka, 1342, Bangladesh

    Md. Rakib Hasan, Nizam Uddin, Tanmoy Sana, Md. Monir Hossain, Milon Mondal & M. S. K. Choudhuri

  2. Department of Pharmacy, Mawlana Bhashani Science and Technology University, Tangail, 1902, Bangladesh

    Md. Monir Hossain

  3. Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka, 1216, Bangladesh

    Ismat Jarin Kanta

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  1. Md. Rakib Hasan
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  2. Nizam Uddin
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  7. M. S. K. Choudhuri
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Correspondence to Md. Monir Hossain.

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Ethical Statement

The study was conducted following the approval by the Institutional Animal Ethical Committee of Jahangirnagar University, Dhaka, Bangladesh [Approval Number: BBECJU/N2016(35)].

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The authors declare that they have no conflict of interest.

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Hasan, M.R., Uddin, N., Sana, T. et al. Analgesic and anti-inflammatory activities of methanolic extract of Mallotus repandus stem in animal models. Orient Pharm Exp Med 18, 139–147 (2018). https://doi.org/10.1007/s13596-018-0312-3

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  • DOI: https://doi.org/10.1007/s13596-018-0312-3

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