Abstract
Recent research has indicated that Long noncoding RNAs (LncRNAs) are crucial in many disorders, especially tumors. However, the exact role of LncRNA XLOC_006786 (LncRNA-SPIDR-2:1) in malignancies, especially in human osteosarcoma, is unclear. The results of RT‒qPCR, western blotting, CCK-8 assays, and Transwell assays showed that LncRNA XLOC_006786 inhibited osteosarcoma cell proliferation, invasion, and migration, indicating that it may be a tumor suppressor gene in osteosarcoma. We found that LncRNA XLOC_006786 negatively regulated NOTCH3, which is an oncogenic gene in osteosarcoma, as we previously reported. Bioinformatics analysis showed that miR-491-5p may be a direct target of LncRNA XLOC_006786, while NOTCH3 is a key target of miR-491-5p. Then, we verified that LncRNA XLOC_006786 could prevent lung metastatic osteosarcoma in vivo. Taken together, our research showed that LncRNA XLOC_006786 suppresses osteosarcoma proliferation, invasion, and metastasis through the NOTCH3 signaling pathway by targeting miR-491-5p.
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The data type used to support the findings of this study are available from the corresponding author upon request.
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Funding
This research is supported by the General Program of Chongqing Natural Science Foundation (No. cstc2019jcyj-msxmX0447) the National Natural Science Foundation of China (Project no.81972519), and Chongqing Research Program of Basic Research and Frontier Technology (no. cstc2019jcyj-msxm1073).
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The human study was approved by the the Medical Ethics Committee of Second Affiliated Hospital of Army Medical University (approval number:2018-069-01). The animal study was approved by the Laboratory Animal Welfare and Ethics Committee Of the Army Medical University (approval number: AMUWEC2019178) and followed the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health.
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Xu, JY., Lv, Yf., Cao, Y. et al. Long noncoding RNA XLOC_006786 inhibits the proliferation, invasion and metastasis of osteosarcoma cells through NOTCH3 signaling pathway by targeting miR-491-5p. Human Cell 36, 2140–2151 (2023). https://doi.org/10.1007/s13577-023-00958-8
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DOI: https://doi.org/10.1007/s13577-023-00958-8