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Long non-coding RNA FGD5-AS1 enhances osteosarcoma cell proliferation and migration by targeting miR-506-3p/RAB3D axis

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Abstract

Osteosarcoma (OSA), the malignant bone tumor, predominantly affecting children and adolescents, threatens the life and life quality of the patients. An increasing number of studies have indicated the role of long non-coding RNA (lncRNA) dysregulation in cancer biology. Herein, the study was aimed to explore the role of FGD5 antisense RNA 1 (FGD5-AS1), a lncRNA, in OSA. Expression levels of FGD5-AS1, miR-506-3p and RAB3D mRNA were quantified utilizing qRT-PCR. The expression of RAB3D protein was examined employing Western blot. A series of functional experiments including CCK-8 assay, BrdU assay, wound healing assay, Transwell assay were performed for studying the effects of FGD5-AS1 on the malignancy of OSA cell lines 143B and HOS. The binding site between miR-506-3p and FGD5-AS1 was identified and validated by luciferase reporter assay and RNA immunoprecipitation assay. It was demonstrated that the expression of FGD5-AS1 was up-regulated in OSA tissues and cell lines, and its high expression is associated with higher Enneking stage and poorer histological differentiation. Gain-of-function and loss-of-function studies suggested that FGD5-AS1 facilitated OSA cells proliferation and migration. The promoting effects of FGD5-AS1 overexpression on OSA cell proliferation and migration could be counteracted by miR-506-3p. Moreover, FGD5-AS1 competitively adsorbed miR-506-3p to repress its expression so as to up-regulate the expression of RAB3D. These results indicate that FGD5-AS1 is capable of expediting OSA cell proliferation and migration via sponging miR-506-3p to up-regulate RAB3D.

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Data availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Funding

This study was supported by Scientific research support fund for Teachers of Jining Medical College. Project Name: Study on the correlation between mir-202-5p site, XIAP and other related proteins in osteosarcoma (ID: JYFC2018FKJ034).

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Authors and Affiliations

  1. Department of Orthopedics, People’s Hospital of Rizhao, Affiliated to Clinical Hospital of Jining Medical University, 126 Tai’an Road, Xinshi District, Rizhao, 276800, Shandong, China

    Congda Li & Bin Wang

  2. People’s Hospital of Rizhao, 126 Taian Rd, Donggang District, Rizhao, Shandong, China

    Xiangbo Lin, Lei Wan & Jijun Yin

  3. Department of Gynaecology, Kuishan Hospital of Rizhao Economic and Technological Development Zone, Rizhao, 276803, Shandong, China

    Caiyun Zhang

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  1. Congda Li
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  2. Xiangbo Lin
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  3. Caiyun Zhang
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  4. Lei Wan
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  5. Jijun Yin
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  6. Bin Wang
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Contributions

BW and CL designed the study. CL and XL conducted most of the experiments and wrote the manuscript. CZ, LW, and JY conducted the experiments and analyzed the data.

Corresponding author

Correspondence to Bin Wang.

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The authors declare that they have no competing interests.

Ethical approval

Our study was approved by the Ethics Review Board of People's Hospital of Rizhao (ID: 201703036).

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Li, C., Lin, X., Zhang, C. et al. Long non-coding RNA FGD5-AS1 enhances osteosarcoma cell proliferation and migration by targeting miR-506-3p/RAB3D axis. Human Cell 34, 1255–1265 (2021). https://doi.org/10.1007/s13577-021-00536-w

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  • DOI: https://doi.org/10.1007/s13577-021-00536-w

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