Abstract
Hepatocellular Carcinoma (HCC) is the second leading cause of cancer-related deaths. Neuroblastoma associated transcript 1 (NBAT1) is a newly identified long noncoding RNA (lncRNA), which has been reported to play an important role in human cancers. However, the functional role and underlying mechanism of NBAT1 in HCC remains unclear. Here, we found that the expression of NBAT1 was decreased in HCC tissues and cells; as well, the decreased expression of NBAT1 was also associated with tumor size and clinical TNM stages. NBAT1 overexpression, both in vitro and in vivo studies, inhibited tumorigenesis through apoptosis augmentation and cell cycle blockade. Mechanistically, NBAT1 bound to IGF2BP1 and inhibited the interaction between IGF2BP1 and c-Myc mRNA, thus suppressing the stability of c-Myc mRNA. Collectively, NBAT1 is associated with HCC tumorigenesis and could be a therapeutic target for HCC treatment.
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This research was supported by Natural Science Fund of the Second Affiliated Hospital of Hainan Medical University.
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This research was supported by Natural Science Fund of the Second Affiliated Hospital of Hainan Medical University.
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This study got the approval of the Ethics Committee of the Second Affiliated Hospital of Hainan Medical University (approval number: 201532-3) and carried out in accordance with the Declaration of Helsinki. Animal experiments were approved by the Institutional Animal Care and Treatment Committee of the Second Affiliated Hospital of Hainan Medical University.
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13577_2020_464_MOESM1_ESM.tif
Supplementary file 1. Supplemental Figure 1. The c-Myc expression is decreased by NBAT1. A. The effect of NBAT1 overexpression on indicated protein in Hep3B cells. B and C. The protein (B) and mRNA (C) expression of c-Myc in tumors formed by control and NBAT1-overexpressed Hep3B cells. D. Control and NBAT1-overexpressed Hep3B cells were treated with CHX (100 μg/ml) for the indicated time points. The cell lysates were subjected to western blot detection and c-Myc expression was quantified by Quantity One software. (TIF 2364 KB)
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Wei, L., Ling, M., Yang, S. et al. Long noncoding RNA NBAT1 suppresses hepatocellular carcinoma progression via competitively associating with IGF2BP1 and decreasing c-Myc expression. Human Cell 34, 539–549 (2021). https://doi.org/10.1007/s13577-020-00464-1
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DOI: https://doi.org/10.1007/s13577-020-00464-1