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MiR-490-5p functions as tumor suppressor in childhood neuroblastoma by targeting MYEOV

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Abstract

Neuroblastoma (NB) is the most common extracranial solid tumor derived from neural crest in children. Recently, the role of miRNA has been studied extensively in the development of NB. Here, we investigated the clinical significance of microRNA-490-5p (miR-490-5p) in NB. A total of 72 pairs of NB tumor tissues and matched adjacent normal nerve tissues were collected from NB patients. The expression of miR-490-5p was significantly down-regulated in NB tissues and cell lines using quantitative real-time PCR. Using Pearson Chi-square test and Kaplan–Meier analysis, we found that significantly decreased miR-490-5p levels were correlated with INSS stage, lymph-node metastasis, and poor survival prognosis in NB patients. MiR-490-5p overexpression significantly suppressed cell proliferation migration, invasion, and induced cell cycle G0/G1 arrest and cell apoptosis in NB cell lines (SH-SY5Y and SK-N-SH) using CCK-8, flow cytometry, and transwell assays. Mechanistically, MYEOV was confirmed as a target gene of miR-490-5p by luciferase reporter assay. Furthermore, MYEOV knockdown imitated, while overexpression rescued the changes in the biological features of miR-490-5p on NB cells. Our results demonstrated for the first time that miR-490-5p functions as a tumor suppressor in NB by targeting MYEOV, which might provide novel approaches for the treatment of NB.

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References

  1. Park JR, Eggert A, Caron H. Neuroblastoma: biology, prognosis, and treatment. Hematol Oncol Clin N Am. 2010;24:65–86.

    Article  Google Scholar 

  2. Wang Z, Yao W, Li K, et al. Reduction of miR-21 induces SK-N-SH cell apoptosis and inhibits proliferation via PTEN/PDCD4. Oncol Lett. 2017;13:4727–33.

    Article  CAS  Google Scholar 

  3. Ahmed AA, Zhang L, Reddivalla N, Hetherington M. Neuroblastoma in children: update on clinicopathologic and genetic prognostic factors. Pediatr Hematol Oncol. 2017;34:165–85.

    Article  Google Scholar 

  4. Ma J, Xu M, Yin M, et al. Exosomal hsa-miR199a-3p promotes proliferation and migration in neuroblastoma. Front Oncol. 2019;9:459.

    Article  Google Scholar 

  5. Park JR, Bagatell R, London WB, et al. Children’s Oncology Group’s 2013 blueprint for research: neuroblastoma. Pediatr Blood Cancer. 2013;60:985–93.

    Article  Google Scholar 

  6. Garaventa A, Parodi S, De Bernardi B, et al. Outcome of children with neuroblastoma after progression or relapse. A retrospective study of the Italian neuroblastoma registry. Eur J Cancer. 2009;45:2835–42.

    Article  Google Scholar 

  7. Croce CM, Calin GA. miRNAs, cancer, and stem cell division. Cell. 2005;122:6–7.

    Article  CAS  Google Scholar 

  8. Berindan-Neagoe I, Monroig Pdel C, Pasculli B, Calin GA. MicroRNAome genome: a treasure for cancer diagnosis and therapy. CA Cancer J Clin. 2014;64:311–36.

    Article  Google Scholar 

  9. Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers. Nature. 2005;435:834–8.

    Article  CAS  Google Scholar 

  10. Cheng X, Xu Q, Zhang Y, et al. miR-34a inhibits progression of neuroblastoma by targeting autophagy-related gene 5. Eur J Pharmacol. 2019;850:53–63.

    Article  CAS  Google Scholar 

  11. Xu Y, Chen X, Lin L, Chen H, Yu S, Li D. MicroRNA-149 is associated with clinical outcome in human neuroblastoma and modulates cancer cell proliferation through Rap1 independent of MYCN amplification. Biochimie. 2017;139:1–8.

    Article  CAS  Google Scholar 

  12. Murray MJ, Raby KL, Saini HK, et al. Solid tumors of childhood display specific serum microRNA profiles. Cancer Epidemiol Biomarkers Prevent. 2015;24:350–60.

    Article  CAS  Google Scholar 

  13. Janssen JWG, Imoto I, Inoue J, et al. MYEOV, a gene at 11q13, is coamplified withCCND1, but epigenetically inactivated in a subset of esophageal squamous cell carcinomas. J Hum Genet. 2002;47:460–4.

    Article  CAS  Google Scholar 

  14. Janssen JW, Vaandrager JW, Heuser T, et al. Concurrent activation of a novel putative transforming gene, myeov, and cyclin D1 in a subset of multiple myeloma cell lines with t(11;14)(q13;q32). Blood. 2000;95:2691–8.

    CAS  PubMed  Google Scholar 

  15. Specht K, Haralambieva E, Bink K, et al. Different mechanisms of cyclin D1 overexpression in multiple myeloma revealed by fluorescence in situ hybridization and quantitative analysis of mRNA levels. Blood. 2004;104:1120–6.

    Article  CAS  Google Scholar 

  16. Moss AC, Lawlor G, Murray D, et al. ETV4 and Myeov knockdown impairs colon cancer cell line proliferation and invasion. Biochem Biophys Res Commun. 2006;345:216–21.

    Article  CAS  Google Scholar 

  17. Lawlor G, Doran PP. MYEOV (myeloma overexpressed gene) drives colon cancer cell migration and is regulated by PGE2. J Exp Clin Cancer Res CR. 2010;29:1–5.

    Article  Google Scholar 

  18. Fang L, Wu S, Zhu X, et al. MYEOV functions as an amplified competing endogenous RNA in promoting metastasis by activating TGF-β pathway in NSCLC. Oncogene. 2019;38(6):896.

    Article  CAS  Google Scholar 

  19. Takita J, Chen Y, Okubo J, et al. Aberrations of NEGR1 on 1p31 and MYEOV on 11q13 in neuroblastoma. Cancer Sci. 2011;102:1645–50.

    Article  CAS  Google Scholar 

  20. Lan G, Yang L, Xie X, Peng L, Wang Y. MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer. AMS. 2015;11:561–9.

    CAS  PubMed  Google Scholar 

  21. Chen K, Zeng J, Tang K, et al. miR-490-5p suppresses tumour growth in renal cell carcinoma through targeting PIK3CA. Biol Cell. 2016;108:41–50.

    Article  CAS  Google Scholar 

  22. Li S, Xu X, Xu X, et al. MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos. Biochem Biophys Res Commun. 2013;441:976–81.

    Article  CAS  Google Scholar 

  23. Yang L, Ma J, Tan Y, et al. Cardiac-specific overexpression of metallothionein attenuates L-NAME-induced myocardial contractile anomalies and apoptosis. J Cell Mol Med. 2019;7:4640–52.

    Google Scholar 

  24. Yamamichi F, Shigemura K, Behnsawy HM, et al. Sonic hedgehog and androgen signaling in tumor and stromal compartments drives epithelial-mesenchymal transition in prostate cancer. Scand J Urol. 2014;48:523–32.

    Article  CAS  Google Scholar 

  25. Chen W, Ye L, Wen D, Chen F. MiR-490-5p inhibits hepatocellular carcinoma cell proliferation, migration and invasion by directly regulating ROBO1. Pathol Oncol Rese POR. 2019;25:1–9.

    Article  Google Scholar 

  26. Xu B, Xu T, Liu H, Min Q, Wang S, Song Q. MiR-490-5p suppresses cell proliferation and invasion by targeting BUB1 in hepatocellular carcinoma cells. Pharmacology. 2017;100:269–82.

    Article  CAS  Google Scholar 

  27. Kai H, Kitadai Y, Kodama M, et al. Involvement of proinflammatory cytokines IL-1beta and IL-6 in progression of human gastric carcinoma. Anticancer Res. 2005;25:709–13.

    CAS  PubMed  Google Scholar 

  28. Janssen JWG, Cuny M, Orsetti B, et al. MYEOV: a candidate gene for DNA amplification events occurring centromeric to CCND1 in breast cancer. Int J Cancer. 2002;102:608.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Jinzhou Medical University First Affiliated Hospital, Jinzhou, 121000, Liaoning, China

    Jinfeng Wang, Hongyue Yao, Yuan Le, Wei Zhou, Jing Li, Lingling Lu, Meixue Chen & Xiang Li

  2. Department of Oncology, Jinzhou Medical University First Affiliated Hospital, No. 2, Section 5, Renmin Street, Guta District, Jinzhou, 121000, Liaoning, China

    Xin Zhang

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  1. Jinfeng Wang
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  2. Xin Zhang
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  3. Hongyue Yao
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  4. Yuan Le
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  5. Wei Zhou
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  6. Jing Li
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  7. Lingling Lu
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  8. Meixue Chen
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  9. Xiang Li
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Correspondence to Xin Zhang.

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All the protocols in this study were approved by the Ethics Committee of Jinzhou Medical University First Affiliated Hospital (Approval number: FM2314-124).

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Wang, J., Zhang, X., Yao, H. et al. MiR-490-5p functions as tumor suppressor in childhood neuroblastoma by targeting MYEOV. Human Cell 33, 261–271 (2020). https://doi.org/10.1007/s13577-019-00302-z

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  • DOI: https://doi.org/10.1007/s13577-019-00302-z

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