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Isolation and expression analysis of eight MADS-box genes in peach (Prunus persica var. nectarina ‘Luxing’)

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Abstract

The MADS-box transcription factor (TF) plays a crucial regulatory role in plant vegetative growth, flower and fruit development. Eight MADS-box genes (designated as PpMADS15, 16, 17, 26, 27, 36, 37, 38; GenBank accession nos. KU559581, KU559582, KU559583, KU559592, KU559593, KU559602, KU559603, KU559604, respectively) were isolated from ‘Luxing’ (Prunus persica var. nectarina ‘Luxing’) peach by homologous comparison and RT-PCR, which contained open reading frames (ORF) of 597, 750, 1062, 615, 699, 1 107, 678 and 564 bp, respectively. The results of phylogenetic analysis revealed that PpMADS15 belonged to the AG subgroup, PpMADS16 to the SEP subgroup, PpMADS17 to the MIKC* group, PpMADS26, 27, and 38 to the Mα group, and PpMADS36 and 37 to the Mγ group. The results of the prediction for subcellular localization showed that eight PpMADS proteins were located in the nucleus. The results of promoter analysis indicated that there were multiple putative cis-acting elements that were involved in responsiveness to the following variables: light, defense and stress, low-temperature, heat stress, wound, fungal elicitor, anaerobic induction, MeJA, gibberellin, ABA, auxin, and SA. RT-PCR results showed that PpMADS15 was expressed in leaves, stems, roots, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS16 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS17 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development (except for 30 d). All members in the Mα and Mγ subgroups were expressed in roots, stems, leaves, sepals, ovaries, stamens, petals and during flower development, but PpMADS27 was expressed only during fruit development. These results suggested that eight PpMADS genes played a crucial regulatory role in vegetative growth, flower and fruit development of peaches.

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Abbreviations

cDNA:

Complementary DNA

ORF:

Opening reading frame

qRT-PCR:

Quantitative real-time PCR

TF:

Transcription factor

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Acknowledgements

This study was supported by National Natural Science Foundation of China (Grant No. 31501742), Shandong Agricultural Good Cultivar Project (Grant No. 2016LZGC034), and Key Research and Development Plan (Major Key Technologies) of Shandong Province (Grant No. 2016ZDJS10A01). I would like to thank Professor Thomas Alan Gavin, Cornell University, and Xu Yi, for help with editing the English in this paper.

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  1. Hui-feng Li and Qing-long Dong have contributed equally to this work.

Authors and Affiliations

  1. Shandong Institute of Pomology, Tai’an, 271000, Shandong, China

    Hui-feng Li, Hou-zhen Jia, Gui-xiang Li & Kun Ran

  2. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A and F University, Yangling, 712100, Shaanxi, China

    Qing-long Dong

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  1. Hui-feng Li
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Correspondence to Kun Ran.

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Li, Hf., Dong, Ql., Jia, Hz. et al. Isolation and expression analysis of eight MADS-box genes in peach (Prunus persica var. nectarina ‘Luxing’). J. Plant Biochem. Biotechnol. 27, 435–442 (2018). https://doi.org/10.1007/s13562-018-0452-z

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