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Effect of subacute treatment with bisphenol A on oxidative stress biomarkers and lipid peroxidation in Gambusia affinis mosquitofish

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Abstract

Objective

Considerable environmental pollution is commonly caused by plastics in marine ecosystems, and as plastics breakdown, many toxic chemicals, including polycarbonate and bisphenol-A, are released, adversely affecting the health of marine organisms. In this regard, the present work was devoted to study the toxic effect of bisphenol A (BPA), an endocrine disruptor, in the freshwater crucian fish Gambusia affinis.

Methods

The acute toxicity of BPA was examined in adult male G. affinis with two lethal concentrations, LC25 and LC50, in which the confidence intervals were determined after 24, 48, 72, and 96 h of the exposure period. The oxidative stress markers, including the enzymatic activity of catalase (CAT), glutathione S-transferase (GST), and acetylcholinesterase (AchE), and the contents of malondialdehyde (MDA) and reduced glutathione (GSH) in the hepatopancreas of control and treated fish were determined for 60 days. In addition, the study was included histopathological examinations of testes.

Result

The toxic effect of BPA exposure increased with exposure time and concentration. Chronic exposure to BPA markedly decreased the levels of reduced glutathione (GSH) and the enzymatic activity of brain acetylcholinesterase (AchE), and in contrast, CAT and GST activity and MDA levels were significantly increased.

Conclusions

BPA-exposed fish exhibited severe concentration-dependent acute toxicity, as evidenced by the induction of oxidative injuries, disruption of the neurotransmission process, and subsequently decreased motor activity and alterations in spermatogenesis.

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Acknowledgements

This research was supported by the National Fund for Scientific Research of Algeria DGSRTD and by the Ministry of Higher Education and Scientific Research of Algeria.

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Author notes

  1. Kheireddine Ouali

    Present address: Laboratory of Environmental Biosurveillance (LBSE), Department of Biology, Faculty of Sciences, Badji-Mokhtar University, BP 12 El Hadjar, 23000, Annaba, Algeria

Authors and Affiliations

  1. Laboratory of Environmental Biosurveillance (LBSE), Department of Biology, Faculty of Sciences, Badji-Mokhtar University, BP 12 El Hadjar, 23000, Annaba, Algeria

    Rahma Belhamra & Fouzia Trea

  2. Environmental Research Center, Sidi Amar Campus, 23000, Annaba, Algeria

    Lazhari Tichati

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  1. Rahma Belhamra
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Rahma BELHAMRA, Lazhari TICHATI, Fouzia TREA, Kheireddine OUALI declare that they have no conflict of interest.

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This article does not contain any studies with human participants. Animal care was carried out in accordance with Badji Mokhtar-Annaba University animal care protocols.

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Belhamra, R., Tichati, L., Trea, F. et al. Effect of subacute treatment with bisphenol A on oxidative stress biomarkers and lipid peroxidation in Gambusia affinis mosquitofish. Toxicol. Environ. Health Sci. 15, 61–72 (2023). https://doi.org/10.1007/s13530-022-00161-6

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