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Investigating the ameliorative effects of the aqueous extract of Pleurotus ostreatus on cyclophosphamide-induced cytotoxicity in rabbits

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Abstract

Objective

This study investigated the ameliorative effect of aqueous extract of Pleurotus ostreatus (APO) against cyclophosphamide-induced cytotoxicity in rabbits.

Methods

Seventy-two healthy rabbits were divided into eight groups each with nine rabbits. Rabbits in groups 1, 2, 3 and 4 (CPA-treated groups) were each given a single oral dose of 50 mg/kg of cyclophosphamide (CPA), however rabbits in groups 2, 3 and 4 were post-treated with 5.0, 7.5 or 10 mg/kg APO on day 1 and throughout the experiment. Rabbits in groups 5, 6, 7 and 8 (CPA-untreated groups) were given only 0.0, 5.0, 7.5 and 10 mg/kg APO per day, respectively throughout the experiment. The experiment lasted 15 days. Serum chemistry analyses and histopathological examination of liver tissues were carried out.

Results

Serum creatinine, bilirubin, globulin and total protein concentrations observed in CPA-treated groups were higher (P < 0.05) than those of CPA-untreated groups. Highest (P < 0.05) values for total protein (83.60 g/dl) and globulin (43.16 g/dl) were recorded in CPA-treated groups administered 10.0 mg/kg APO per day. Haematological measurements (packed cell volume, haemoglobin, white blood cell, platelet and red blood cells) in CPA-treated groups were lower (P < 0.01) in comparison to those of CPA-untreated groups. However, an increase (P < 0.01) in the values of these parameters was observed following post-treatment of rabbits in the CPA-treated groups with 7.5 and 10.0 mg/kg APO per day.

Conclusion

The findings of this study thus indicate that aqueous extract of Pleurotus ostreatus has the potential to ameliorate CPA-induced renal injury and myelosuppression in a dose-dependent manner.

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References

  1. Malet-Martino M, Gilard V, Martino R (1999) The analysis of cyclophosphamide and its metabolites. Curr Pharm Des 5(8):561–586

    CAS  PubMed  Google Scholar 

  2. Shanafelt TD, Lin T, Geyer SM (2007) Pentostatin, cyclophosphamide and rituximab regimen in older patients with chronic lymphocytic leukemia. Cancer 109(11):2291–2298

    CAS  PubMed  Google Scholar 

  3. Zhang QH, Wu CF, Duan L, Yang JY (2008) Protective effects of ginsenoside Rg 3 against cyclophosphamide-induced DNA damage and cell apoptosis in mice. Arch Toxicol 82(2):117–123

    CAS  PubMed  Google Scholar 

  4. Singh G, Fries JF, Williams CA, Zatarain E, Spitz P, Bloch DA (1991) Toxicity profiles of disease modifying antirheumatic drugs in rheumatoid arthritis. J Rheumatol 18(2):188–194

    CAS  PubMed  Google Scholar 

  5. Moore MJ (1991) Clinical pharmacokinetics of cyclophosphamide. Clin Pharmacokinet 20(3):194–208

    CAS  PubMed  Google Scholar 

  6. Langford CA, Klippel JH, Balow JE, James SP, Sneller MC (1998) Use of cytotoxic agents and cyclosporine in the treatment of autoimmune disease: part 1: rheumatologic and renal diseases. Ann Intern Med 128(12):1021–1028

    CAS  PubMed  Google Scholar 

  7. Lohrmann HP (1984) The problem of permanent bone marrow damage after cytotoxic drug treatment. Oncology 41(3):180–184

    CAS  PubMed  Google Scholar 

  8. Ray S, Sengupta C, Roy K (2005) Evaluation of ascorbic acid as suppressor of cyclophosphamide induced lipid peroxidation using common laboratory markers. Acta Pol Pharm 62(2):145–152

    CAS  PubMed  Google Scholar 

  9. Pryor BD, Bologna SG, Kahl LE (1996) Risk factors for serious infection during treatment with cyclophosphamide and high-dose corticosteroids for systemic lupus erythematosus. Arthritis Rheum 39(9):1475–1482

    CAS  PubMed  Google Scholar 

  10. Jagadish LK, Shenbhagaraman R, Venkatakrishan V, Kaviyarasan V (2008) Studies on the phytochemical, antioxidant and antimicrobial properties of three indigenous Pleurotus species. J Mol Biol Biotechnol 2(1):20–29

    Google Scholar 

  11. Nehra K, Meenakshi MK, Ajay Y (2012) Evaluation of antimicrobial potential of fruiting body extracts of Pleurotus ostreatus (oyster mushroom). Int J Microbial Resour Technol 1(4):391–400

    Google Scholar 

  12. Deepalakshmi K, Mirulani S (2014) Toxicological assessment of Pleurotus ostreatus in Sprague Dawley Rats. Int J Nutr Pharmacol Neurol Dis 4:139–145

    Google Scholar 

  13. Sher H, Alyemeni M, Bahkali A, Sher H (2010) Effect of environmental factors on the yield of selected mushroom species growing in two different agro ecological zones of Pakistan. Saudi J Biol Sci 17(4):3211–3326

    Google Scholar 

  14. Mattila P, Salo-Vaananen P, Konko K, Aro H, Jalava T (2002) Basic composition and amino acid contents of mushrooms cultivated in Finlands. J Agric Food Chem 50:6419–6422

    CAS  PubMed  Google Scholar 

  15. Chirinang P, Intarapichet K (2009) Amino acids and antioxidant properties of the oyster mushrooms, Pleurotus ostreatus and Pleurotus sajor-caju. Sci Asia 35:326–333

    CAS  Google Scholar 

  16. Moore DM (2000) Haematology of rabbits and haematology of the guinea pig. In: Feldman BF, Zinki JG, Jain NC (eds) Schalm's veterinary haematology, 5th edn. Lippincott Williams & Wilkins, pp 1100–1110

  17. Harcourt-Brown F (2002) The textbook of rabbit medicine, 4th edn. Butterworth Heinemann Education, Oxford, pp 28–30

    Google Scholar 

  18. Archetti I, Tittarelli C, Cerioli M, Brivio R, Grilli G, Lavazza A (2008) Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial farms in Northern Italy. 9. Proceedings of the 9th World Rabbit Congress, 10–13 June 2008, Verona, Italy, pp 1147–1152

  19. Sirag HM (2009) Biochemical and hematological studies for the protective effect of oyster mushroom (Pleurotus ostreatus) against glycerol-induced acute renal failure in rats. J Biol Sci 9:746–752

    Google Scholar 

  20. Naguib YM, Azmy RM, Samaka RM, Salem MF (2014) Pleurotus ostreatus opposes mitochondrial dysfunction and oxidative stress in acetaminophen-induced hepato-renal injury. BMC Complement Altern Med 15(14):494

    Google Scholar 

  21. Ahmed RA (2018) Hepatoprotective and antiapoptotic role of aged black garlic against hepatotoxicity induced by cyclophosphamide. JOBAZ 79:8

    Google Scholar 

  22. Maruo Y, Sato H, Bamba N, Iwai M, Sawa H, Fujino H, Taga T, Ota S, Shimada MJ (2001) Chemotherapy-induced unconjugated hyperbilirubinemia caused by a mutation of the bilirubin uridine-5′-diphosphate-glucuronosyltransferase gene. Pediatr Hematol Oncol 23(1):45–47

    CAS  Google Scholar 

  23. Matsuoka Y, Masuda H, Yokoyama M, Kihara K (2008) Protective effects of bilirubin against cyclophosphamide induced hemorrhagic cystitis in rats. J Urol 179(3):1160–1166

    CAS  PubMed  Google Scholar 

  24. Viswanatha AHM, Swamy UM, Patel BC, Koti PC, Gadad NL, Hippeswamy AHM (2013) Cardioprotective effect of Saraca indica against cyclophosphamide induced cardiotoxicity in rats: a biochemical, electrocardiographic and histopathological study. Indian J Pharmacol 45(1):44–48

    Google Scholar 

  25. Martínez-Gabarrón M, Enríquez R, Sirvent AE, García-Sepulcre M, Millán I, Amorós F (2011) Hepatotoxicity following cyclophosphamide treatment in a patient with MPO-ANCA vasculitis. Nefrologia 31:496–498

    PubMed  Google Scholar 

  26. Fatani AG, Darweesh AQ, Rizwan L, Aleisa AM, Al-Shabanah OA, Sayed-Ahmed MM (2010) Carnitine deficiency aggravates cyclophosphamide-induced cardiotoxicity in rats. Chemotherapy 56(1):71–81

    CAS  PubMed  Google Scholar 

  27. Refaie FM, Esmat YA, Daba AS, Osman WM, Taha SM (2010) Hepatoprotective activity of polysaccharopeptides from Pleurotus ostreatus mycelium on thioacetamide-intoxicated mice. Micol Apl Int 22:1–13

    Google Scholar 

  28. Mueller-Eckhardt C, Küenzlen E, Kiefel V (1983) Cyclophosphamide-induced immune thrombocytopenia in a patient with ovarian carcinoma successfully treated with intravenous gamma globulin. Blut 46(3):165–169

    CAS  PubMed  Google Scholar 

  29. Jayakumar T, Ramesh E, Geraldine P (2006) Antioxidant activity of the oyster mushroom, Pleurotus ostreatus, on CCL(4)-induced liver injury in rats. Food Chem Toxicol 44(12):1989–1996

    CAS  PubMed  Google Scholar 

  30. Lee J, Lim KT (2013) Protection against cyclophosphamide-induced myelosuppression by ZPDC glycoprotein (24 kDa). Immunol Invest 42(1):61–80

    CAS  PubMed  Google Scholar 

  31. Chen CA, Chen M (2003) Simultaneous occurrence of hepatic focal nodular hyperplasia and uterine endometrial stromal nodule in a patient having treated breast infiltrating ductal carcinoma. Acta Obstet Gynecol Scand 82(6):585–586

    PubMed  Google Scholar 

  32. Basaranoglu M, Basaranoglu G, Bugianesi E (2015) Carbohydrate intake and nonalcoholic fatty liver disease: fructose as a weapon of mass destruction. Hepatobil Surg Nutr 4(2):109–116

    Google Scholar 

  33. Kneeman JM, Misdraji J, Corey KE (2012) Secondary causes of nonalcoholic fatty liver disease. Ther Adv Gastroenterol 5(3):199–207

    Google Scholar 

  34. McDonald GB, Slattery JT, Bouvier ME, Ren S, Batchelder AL, Kalhorn TF, Schoch HG, Anasetti C, Gooley T (2003) Cyclophosphamide metabolism, liver toxicity, and mortality following hematopoietic stem cell transplantation. Blood 101(5):2043–2048

    CAS  PubMed  Google Scholar 

  35. Poonam GD, Appasaheb A, Ghadge SB, Tannaz JB (2011) Preparation of decoction of medicinal plants: a self-help measure? J Altern Complement Med 17(12):1099–1100

    Google Scholar 

  36. Tahouri H, Padide, D (2011) The encyclopedia of medicinal plants, 2nd edn. In: Persian Qom, pp 365–366

  37. Dandapat S, Kumar M, Sinha MP (2014) Pharmacological and phytochemical screening of Aegle marmelos (L.) and Cinnamomum tamala (Boch.-Ham.) leaves for therapeutic efficacy. Middle East J Sci Res 22:626–632

    Google Scholar 

  38. Essien JP, Odoemena CS (1995) Antibacterial activity of the root extract of Telfairia occidentalis (fluted pumpkin). West Afr J Biol Appl Chem 40:1–4

    Google Scholar 

  39. Adejumo TO, Awosanya OB (2004) Proximate and mineral composition of four edible mushroom species from South Western Nigeria. Afr J Biotechnol 4(10):1084–1088

    Google Scholar 

  40. Malick CP, Singh MB (1980) Plant enzymology and histoenzymology. Kalyani Publishers, New Delhi

    Google Scholar 

  41. Helmja K, Vaher M, Gorbatsova J, Kaljurand M (2007) Characterization of bioactive compounds contained in vegetables of the solanaceae family by capillary electrophoresis. Proc Est Acad Sci Chem 56:172–186

    CAS  Google Scholar 

  42. Harbone JB (1973) Phytochemical methods: a guide to modern technique of plant analysis, 2nd edn. Chapman and Hall, New York

    Google Scholar 

  43. AOAC (1980) Official methods of analysis. Association of Analytical Chemists, Arlington

    Google Scholar 

  44. National Research Council (NRC) (1996) Guide for the care and use of laboratory animals, 7th edn. National Academy Press, Washington

    Google Scholar 

  45. Reitman S, Frankel SA (1957) Colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol 28:56–63

    CAS  PubMed  Google Scholar 

  46. Gerhardt W, Waldenstrom G (1979) Creatine kinase B-subunit activity in serum after immunoinhibition of M-subunit activity. Clin Chem 25:1274–1279

    CAS  PubMed  Google Scholar 

  47. Rodrigues EB, Costa EF, Penha FM, Melo GB, Bottós J, Dib E, Furlani B, Lima VC, Maia M, Meyer CH, Höfling-Lima AL, Farah ME (2009) The use of vital dyes in ocular surgery. Surv Ophthalmol 54(5):576–617

    PubMed  Google Scholar 

  48. SAS Institute Inc (2004) SAS® data quality server 9.1: reference. SAS Institute Inc., Cary, NC

    Google Scholar 

Download references

Acknowledgements

The authors are grateful to the management and staff of Bioresources Development Centre, Isanlu, Kogi State, Nigeria, who provided the laboratory facilities needed for this investigative study.

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Authors and Affiliations

  1. National Biotechnology Development Agency, Abuja, 900101, Nigeria

    Adekunle B. Rowaiye, Suliat A. Salami, Titilayo M. Asala & Augustine Aiyedoju

  2. Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

    Temidayo Omobowale

  3. Bioresources Development Centre Isanlu, National Biotechnology Development Agency, Abuja, Nigeria

    Solomon O. Oni, Sonny O. Peters, Rukayat O. Bukoye & John E. Ukpong

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  1. Adekunle B. Rowaiye
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Correspondence to Suliat A. Salami.

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Ethical statement

“All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.” All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Conflict of interest

Adekunle B. Rowaiye, Temidayo Omobowale, Suliat A. Salami, Titilayo M. Asala, Solomon O. Oni, Augustine Aiyedoju, Sonny O. Peters, Rukayat O. Bukoye, John E. Ukpong declare that they have no conflict of interest.

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Rowaiye, A.B., Omobowale, T., Salami, S.A. et al. Investigating the ameliorative effects of the aqueous extract of Pleurotus ostreatus on cyclophosphamide-induced cytotoxicity in rabbits. Toxicol. Environ. Health Sci. 12, 213–225 (2020). https://doi.org/10.1007/s13530-020-00014-0

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