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Study on the expression of CD14 + CD16 + monocytes and VEGF in peripheral blood of patients with type 2 diabetes mellitus and diabetic macroangiopathy

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International Journal of Diabetes in Developing Countries Aims and scope Submit manuscript

Abstract

Objectives

To study the expression of CD14 + CD16 + monocytes and VEGF and the levels of serum adiponectin and MCP-1 in peripheral blood of patients with type 2 diabetes mellitus (T2DM) and diabetic macroangiopathy to understand the possible mechanism of inflammatory immune response in T2DM and diabetic macroangiopathy.

Methods

Peripheral blood CD14 + CD16 + monocytes were detected by flow cytometry in 50 T2DM patients, 50 patients with diabetic macroangiopathy, and 20 healthy controls or normal controls who participated in outpatient physical examination, and used the Ficoll-Hypaque density gradient centrifugation isolated PBMC and quantitative PCR technology comparison between groups research object in the peripheral blood PBMC VEGF mRNA expression level. Serum levels of adiponectin and MCP-1 were measured by ELISA.

Results

Compared with normal control group (NGT), the fluorescence intensity of proinflammatory CD14 + CD16 + monocytes in simple T2DM group and T2DM combined with macroangiopathy group were significantly increased (p < 0.05), and T2DM combined with macroangiopathy group was significantly higher than T2DM group, the difference was statistically significant (p < 0.01). Moreover, the expression of VEGF mRNA in peripheral blood PBMCs, T2DM combined with macroangiopathy> T2DM patients> healthy volunteers, the differences were significant (p < 0.05). Compared with NGT group, the levels of serum adiponectin in T2DM group and T2DM combined with macroangiopathy group were significantly lower than those in NGT group (p < 0.01), and the levels of serum adiponectin, simple T2DM Group were lower than T2DM combined with macroangiopathy group (p < 0.05). The level of MCP-1 in serum compared with simple T2DM group and NGT group, T2DM combined with macroangiopathy group had statistically significant difference (p < 0.05). The serum level of MCP-1 in T2DM group was also higher than that in NGT group (p < 0.05). At the same time, we also found that the increase of CD14 + CD16 + monocytes was positively correlated with serum MCP-1 levels.

Conclusions

T2DM patients and T2DM combined with macroangiopathy patients have increased expression of VEGF and MCP-1 concentration in peripheral blood mononuclear cells. The increase of MCP-1 may increase the number of CD14 + CD16 + monocytes, which is involved in the chronic inflammation in patients with T2DM and T2DM combined with macroangiopathy, resulting in the occurrence and development of T2DM and its complications.

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Funding

This study is funded by the National Natural Science Foundation of China (NO.81560147), The Science and Technology Support Program of Guizhou Province (Contract No. Qian Ke He Supporting Project [2017]2884]), The Key Science and Technology Program of Guizhou Province [Contract No. Qian Ke He SY (2012) 3116], and The Science and Technology Research Foundation of Guizhou Province, China [Contract No. Qian Ke He J LKZ (2013) 53].

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Authors and Affiliations

  1. Department of Endocrinology, Zunyi Medical University, Zunyi, 563000, Guizhou, China

    Xue Zhou, Meng-Xue Yang, Bo Yang, Bo-Wen Sun & Fei Li

  2. Department of Endocrinology, Shanghai Fifth People’s Hospital, Fudan University, Shanghai, 200240, China

    Meng-Xue Yang

  3. School of Public Health, Zunyi Medical University, Zunyi, 563000, Guizhou, China

    Jie Xu & Jie Yu

  4. Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, 563006, Guizhou, China

    Qi-Hai Gong

  5. Department of Nephrology, The First Affiliated Hospital of Zunyi Medical University, Zunyi, 563000, Guizhou, China

    Xiao-Yong Yan, Yi-Bin Yang & Mei Tian

Authors
  1. Xue Zhou
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  2. Meng-Xue Yang
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  3. Jie Xu
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  4. Jie Yu
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  5. Bo Yang
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  7. Fei Li
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  9. Xiao-Yong Yan
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  10. Yi-Bin Yang
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  11. Mei Tian
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Correspondence to Meng-Xue Yang.

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The study was approved by the patient and by the Ethics Committee of Zunyi Medical University.

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Zhou, X., Yang, MX., Xu, J. et al. Study on the expression of CD14 + CD16 + monocytes and VEGF in peripheral blood of patients with type 2 diabetes mellitus and diabetic macroangiopathy. Int J Diabetes Dev Ctries 40, 507–512 (2020). https://doi.org/10.1007/s13410-020-00809-4

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  • DOI: https://doi.org/10.1007/s13410-020-00809-4

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