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Frequency of MRSA in diabetic foot infections

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Abstract

Staphylococcus aureus is one of the most common bacterial pathogens isolated from diabetic foot infections (DFIs). The increasing prevalence of meticillin-resistant S. aureus (MRSA) in patients with diabetes is associated with complications. The aim of this study was to determine the prevalence of S. aureus in DFIs and antibiotic susceptibility patterns of MRSA and non-MRSA isolates. Identification of S. aureus and MRSA was performed by the phenotypic and molecular methods. The Kirby-Bauer and agar dilution methods were performed for determination of antibiotic susceptibility patterns. Thirty-four isolates of S. aureus were isolated from March 2014 to February 2015. The rate of MRSA was 38.23 % according to the disk cefoxitin and oxacillin agar dilution methods, and as by PCR method 12) 35.29 %), isolates were found to have the mecA gene. All MRSA and non-MRSA isolates were susceptible to linezolid and vancomycin. The resistance rate to ceftriaxone was high followed by amoxicillin-clavulanic acid, tetracycline, gentamicin, and erythromycin. The most common bacterial pathogen isolated from DFIs was S. aureus. To ensure effective treatment, accurate detection of MRSA is critical. Our findings showed that MRSA isolates had high-level resistance to antimicrobial agents and that appropriate antibiotic therapy, based on the antibiotic susceptibility pattern, is essential to ensure a good result.

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Acknowledgments

This research was supported by a grant from Infectious and Tropical Disease Research Center of Tabriz University of Medical Sciences (TUMS), and the manuscript was written based on a dataset of the M.Sc. thesis of Naser Alizadeh registered at Tabriz University of Medical Sciences (Number: 11017-May 2014). The authors would like to thank the staff of Imam Reza and Sina infectious disease wards and microbiology department for their help.

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Authors and Affiliations

  1. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Taghi Akhi, Mojtaba Varshochi & Naser Alizadeh

  2. Department of Bacteriology and Virology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran

    Mohammad Taghi Akhi, Reza Ghotaslou, Mohammad Yousef Memar, Tahereh Pirzadeh & Naser Alizadeh

  3. Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Asgharzadeh

Authors
  1. Mohammad Taghi Akhi
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  2. Reza Ghotaslou
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  3. Mohammad Yousef Memar
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  4. Mohammad Asgharzadeh
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  5. Mojtaba Varshochi
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  6. Tahereh Pirzadeh
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  7. Naser Alizadeh
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Correspondence to Naser Alizadeh.

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This study was approved by The Ethic Commission of Tabriz University of Medical Sciences (Number: 5/4/589 -23 Mar. 2014).

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The authors declare that they have no conflict of interests.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional.

Grant

This research was supported by a grant from Research center of infectious and tropical disease of Tabriz University of Medical sciences.

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Akhi, M.T., Ghotaslou, R., Memar, M.Y. et al. Frequency of MRSA in diabetic foot infections. Int J Diabetes Dev Ctries 37, 58–62 (2017). https://doi.org/10.1007/s13410-016-0492-7

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  • DOI: https://doi.org/10.1007/s13410-016-0492-7

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