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Ultrasound-assisted extraction of phytochemicals from Eryngium foetidum leaves using response surface methodology

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Abstract

This study was intended to optimize ultrasound-assisted extraction conditions of the bioactive compounds present in Eryngium foetidum leaves using response surface methodology. Initially, four different solvents (acetone, methanol, ethanol and distilled water) were taken to extract the phytochemicals using ultrasound. Experimentally, acetone was found to be the best solvent for extracting the bioactive compounds because of higher recovery of phenolic compounds with the value of 20 mg GAE/g of sample in contrast to other solvent. The ranges of independent variables were selected on the basis of maximum phenolic content achieved. For optimization, Box–Behnken design with three independent variables, viz. extraction time of 5–15 min, ultrasound amplitude of 40–80% and solvent concentration of 40–80%, was selected. Three dependent variables, viz. total phenolic content, total flavonoids content and DPPH radical scavenging activity, were selected for the study. Quadratic model was found to be the best fit for all the responses. The optimized condition was extraction time of 12 min, amplitude of 61 % and solvent concentration of 80%, yielding the maximum yield of responses with the desirability value of 0.96. The results indicate that Eryngium foetidum leaves are potent source of phytochemicals.

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Acknowledgements

The author wants to acknowledge the UGC-SAP, Tezpur University, and IKGPTU, Kapurthala, for the instrument facilities.

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Authors and Affiliations

  1. Department of Food Science and Technology, I.K. Gujral Punjab Technical University, Kapurthala, Punjab, 144603, India

    Shubham Rohilla, Akriti Jaiswal & Barinderjit Singh

  2. Department of Food Engineering and Technology, Tezpur University, Tezpur, Assam, 784028, India

    Shubham Rohilla & Charu Lata Mahanta

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Contributions

Shubham Rohilla: conceptualization; data curation; formal analysis; investigation; methodology; validation; visualization; and roles/writing, original draft.

Akriti Jaiswal: formal analysis; visualization; and roles/writing, original draft.

Barinderjit Singh: data validation; visualization; conceptualization, and review and editing.

Charu Lata Mahanta: methodology; resources; supervision; validation; visualization; and writing, review and editing.

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Correspondence to Shubham Rohilla.

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Rohilla, S., Jaiswal, A., Singh, B. et al. Ultrasound-assisted extraction of phytochemicals from Eryngium foetidum leaves using response surface methodology. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04784-8

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